EMAGE mouse gene expression spatial database

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EMAGE:15245

Slc1a1 solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 ( MGI:105083)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010393. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010393_01	euxassay_010393_02	euxassay_010393_03	euxassay_010393_04

euxassay_010393_05	euxassay_010393_06	euxassay_010393_07	euxassay_010393_08	euxassay_010393_09

euxassay_010393_10	euxassay_010393_11	euxassay_010393_12	euxassay_010393_13	euxassay_010393_14

euxassay_010393_15	euxassay_010393_16	euxassay_010393_17	euxassay_010393_18	euxassay_010393_19

euxassay_010393_20	euxassay_010393_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15245_wholemount_strong.wlz

15245_wholemount_moderate.wlz

15245_wholemount_weak.wlz

15245_wholemount_possible.wlz

15245_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15245_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30512
Entity Detected:	Slc1a1, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 ( MGI:105083)
Sequence:	sense strand is shown >T30512 TGACCATGGTGATCGTGCTGAGTGCTGTGGGGCTGCCTGCCGAGGACGTCACCCTGATCATTGCTGTTGA CTGGCTCCTGGACCGGTTCAGGACCATGGTGAACGTCCTGGGTGATGCGTTTGGGACGGGCATCGTAGAG AAGCTCTCGAAGAAGGAGCTGGAGCAGATGGATGTTTCGTCTGAAGTCAACATCGTGAACCCCTTTGCCT TGGAACCCACAACCCTCGATAACGAAGACTCAGATACCAAGAAGTCTTATGTCAATGGGGGCTTCGCGGT AGACAAATCTGACACCATCTCGTTCACTCAGACCTCACAGTTCTAGATGCCTGACCTCAGATTGAGGCCT GGGATTGTGAAGGGCGTCTCCACAGGAGCCATCTCCTAGCAAACTCCGACATTAGGGAACGGGAAGGACA CTAAGAGTCAACTGTACATTTAGTTTGATAAACAGACCTCCAGATTATTTTCTATATTTGACTTTATAGC CTTGGTTCTCTGGGTTTAGGGATTTGGGGTGAGATGAACTGAAAGGAAATTAAGAAAGTTGTGTTATCTG GATTTTCTAATTCTATACAACAGAGTTTGGAAGTATATGAAGTAGTAACTGTTAGGATTAGGTCATAGAT ATGGAAGAGAAATTGGTTTCTCATGCATAGACCAGTGTTTGGGGTTTTTAAACAATATTATTGGCTACAA ATTTTTACTCAGGCTTTCTATTGGCAGGACTTCCCTTGCCTTTTTACTTTTATAGATTATAATGCATCTC AAAAGCCCTACCCAGTTAATGTGCCAAATTTTCCATTTTGACCTCATCTCCAGCCACTCTCAAACTACCC TGGGGCGGGGGGAGCAAAAAAGATCAGCATAGTTCTGCAATAACAGTTTAAAGATAGTTGTGGGGTTTAG GGGAAGGGAAAGGGTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30029501), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60629. Forward Primer - name:060629_F_IRAV109_g01_Slc1a1, sequence:TGACCATGGTGATCGTGC; Reverse Primer - name:060629_R_SP6_IRAV109_g01_Slc1a1, sequence:AAACCCTTTCCCTTCCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15241 same embryo
	EMAGE:15242 same embryo
	EMAGE:15243 same embryo
	EMAGE:15244 same embryo
	EMAGE:15246 same embryo
	EurExpress:euxassay_010393 same experiment
	MGI:4828124 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source