EMAGE mouse gene expression spatial database

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EMAGE:15253

Slc30a5 solute carrier family 30 (zinc transporter), member 5 ( MGI:1916298)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010448. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010448_01	euxassay_010448_02	euxassay_010448_03	euxassay_010448_04

euxassay_010448_05	euxassay_010448_06	euxassay_010448_07	euxassay_010448_08	euxassay_010448_09

euxassay_010448_10	euxassay_010448_11	euxassay_010448_12	euxassay_010448_13	euxassay_010448_14

euxassay_010448_15	euxassay_010448_16	euxassay_010448_17	euxassay_010448_18	euxassay_010448_19

euxassay_010448_20	euxassay_010448_21	euxassay_010448_22	euxassay_010448_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15253_wholemount_strong.wlz

15253_wholemount_moderate.wlz

15253_wholemount_weak.wlz

15253_wholemount_possible.wlz

15253_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15253_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 18 19 20 weak expression: see section 06 17 21

submandibular gland primordium

moderate

regional

moderate expression: see section 07 17 weak expression: see section 06 08 15 16 18 not examined expression: see section 09 19

mandible

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 19 20 21 weak expression: see section 09 10 15 16 17 18 22

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 08 20 weak expression: see section 16 17 18

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 21 22 23 weak expression: see section 05 06 07 08 18 19 20

viscerocranium

weak

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 07 08 09 15 16 weak expression: see section 05 06 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30206
Entity Detected:	Slc30a5, solute carrier family 30 (zinc transporter), member 5 ( MGI:1916298)
Sequence:	sense strand is shown >T30206 TCCAAATGTGCCCGCTATGGGTCCTTTCCCATTTTTATTAGTGCCCTCCTGTTTGGAAATTTCTGGACAC ACCCAATAACAGACCAACTCCGGGCTATGAACAGAGCAGCACACCAGGAGAGCACAGAGCACGTCCTGTC TGGAGGAGTGGTAGTGAGCGCTGTGTTCTTCATTTTGTCGGCCAACATTCTATCATCTCCCTCTAAGAGA GGACAGAAAGGTACCCTTATTGGATATTCTCCTGAAGGAACACCACTCTATCACTTCATGGGGGACGCTT TTCAGCACAGCTCTCAGTCGGTGCCTAGGTTCATTAAGGACTCACTAAAGCAGGTTCTCGAGGAGAGCGA CTCTAGGCAGATCTTTTACTTCTTGTGCTTGAATCTGCTTTTTACCTTTGTGGAGTTGTTCTATGGCGTG CTAACCAACAGTCTAGGCCTGATCTCAGATGGATTTCACATGCTCTTTGACTGCTCGGCTTTGGTCATGG GACTGTTTGCTGCCCTGATGAGCCGCTGGAAAGCCACCCGGATTTTCTCCTATGGGTATGGCCGAATAGA GATTCTCTCTGGCTTTATTAATGGGCTTTTTCTGATCGTGATAGCATTTTTTGTGTTTATGGAATCAGTG GCTAGACTGATCGATCCTCCGGAACTAGACACAAACATGCTGACACCAGTTTCCGTCGGAGGGCTGATAG TAAACCTTATTGGTATCTGTGCCTTCAGCCACGCCCACAGCCATGGCCATGGCGCTTCTCAAGGAAACTG CCACTCTGATCACGGCCATTCACACCATGCACATGGACACGGCCATGATCACGGTCACAGCCACGGCTTC ACGGGTGGAGGCATGAATGCGAACATGAGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3156622), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21155. Forward Primer - name:021155_F_IRAV51-54_I06_Slc30a5, sequence:TCCAAATGTGCCCGCTAT; Reverse Primer - name:021155_R_SP6_IRAV51-54_I06_Slc30a5, sequence:CCCCTCATGTTCGCATTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15254 same embryo
	EMAGE:15257 same embryo
	EMAGE:15256 same embryo
	EMAGE:15255 same embryo
	EMAGE:15252 same embryo
	EurExpress:euxassay_010448 same experiment
	MGI:4828191 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source