EMAGE mouse gene expression spatial database

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EMAGE:15266

Fgfr4 fibroblast growth factor receptor 4 ( MGI:95525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010371. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010371_01	euxassay_010371_02	euxassay_010371_03	euxassay_010371_04

euxassay_010371_05	euxassay_010371_06	euxassay_010371_07	euxassay_010371_08	euxassay_010371_09

euxassay_010371_10	euxassay_010371_11	euxassay_010371_12	euxassay_010371_13	euxassay_010371_14

euxassay_010371_15	euxassay_010371_16	euxassay_010371_17	euxassay_010371_18	euxassay_010371_19

euxassay_010371_20	euxassay_010371_21	euxassay_010371_22	euxassay_010371_23	euxassay_010371_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15266_wholemount_strong.wlz

15266_wholemount_moderate.wlz

15266_wholemount_weak.wlz

15266_wholemount_possible.wlz

15266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 17 18 19 20 21 22 23 24

upper leg muscle

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 23 24

foot

moderate

regional

moderate expression: see section 06 22

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 23 24

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

eye skeletal muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22 23 24

naris

weak

regional

weak expression: see section 15 16 17 18

viscerocranium

moderate

regional

Expression in the turbinate bone.

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 weak expression: see section 17 18

midgut

moderate

regional

moderate expression: see section 10 13 14 15 16 17 weak expression: see section 11 12

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30233
Entity Detected:	Fgfr4, fibroblast growth factor receptor 4 ( MGI:95525)
Sequence:	sense strand is shown >T30233 TCCACGGGGAGAATCGTATTGGAGGCATTCGGCTGCGCCACCAACACTGGAGCCTGGTGATGGAAAGTGT GGTACCCTCGGACCGTGGCACATACACATGCCTTGTGGAGAACTCTCTGGGTAGCATTCGCTACAGCTAT CTCCTGGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTCCCAGCCAACACCACAG CTGTGGTTGGCAGCGATGTGGAGCTACTCTGCAAGGTGTACAGCGACGCCCAGCCCCACATACAGTGGCT GAAACACGTCGTCATCAACGGCAGCAGCTTCGGCGCCGACGGTTTCCCCTACGTACAAGTCCTGAAGACA ACAGACATCAATAGCTCGGAGGTAGAGGTCTTGTATCTGAGGAACGTGTCCGCTGAGGATGCAGGAGAGT ATACCTGTCTGGCGGGCAACTCCATCGGCCTTTCCTACCAGTCAGCGTGGCTCACGGTGCTGCCAGAGGA AGACCTCACGTGGACAACAGCAACCCCTGAGGCCAGATACACAGATATCATCCTGTATGTATCAGGCTCA CTGGTTCTGCTTGTGCTCCTGCTGCTGGCCGGGGTGTATCATCGGCAAGTCATCCGTGGCCACTACTCTC GCCAGCCTGTCACTATACAAAAGCTGTCCCGTTTCCCTTTGGCCCGACAGTTCTCTTTGGAGTCGAGGTC CTCTGGCAAGTCAAGTTTGTCCCTGGTGCGAGGTGTCCGTCTCTCCTCCAGCGGCCCGCCCTTGCTCACG GGCCTTGTGAATCTAGACCTGCCTCTCGATCCGCTTTGGGAATTCCCCCGGGACAGGTTGGTGCTCGGAA AGCCCCTGGGTGAGGGCTGCTTTGGGCAAGTGGTTCGTGCAGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5132831), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 25401. Forward Primer - name:025401_F_IRAV59-62_K22_Fgfr4, sequence:TCCACGGGGAGAATCGTA; Reverse Primer - name:025401_R_SP6_IRAV59-62_K22_Fgfr4, sequence:GCCTCTGCACGAACCACT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15267 same embryo
	EMAGE:15265 same embryo
	EMAGE:15268 same embryo
	EMAGE:15264 same embryo
	EMAGE:15269 same embryo
	EurExpress:euxassay_010371 same experiment
	MGI:4824849 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source