EMAGE mouse gene expression spatial database

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EMAGE:15268

Cmtm4 CKLF-like MARVEL transmembrane domain containing 4 ( MGI:2142888)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010370. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010370_01	euxassay_010370_02	euxassay_010370_03	euxassay_010370_04

euxassay_010370_05	euxassay_010370_06	euxassay_010370_07	euxassay_010370_08	euxassay_010370_09

euxassay_010370_10	euxassay_010370_11	euxassay_010370_12	euxassay_010370_13	euxassay_010370_14

euxassay_010370_15	euxassay_010370_16	euxassay_010370_17	euxassay_010370_18	euxassay_010370_19

euxassay_010370_20	euxassay_010370_21	euxassay_010370_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15268_wholemount_strong.wlz

15268_wholemount_moderate.wlz

15268_wholemount_weak.wlz

15268_wholemount_possible.wlz

15268_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15268_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 15 16 17

pancreas

weak

regional

weak expression: see section 07 08 09 10 11 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

vomeronasal organ

moderate

regional

moderate expression: see section 14

stomach

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09

rectum

weak

regional

weak expression: see section 14

midgut

weak

regional

weak expression: see section 11 12 13 14 15 16 17

bladder

weak

regional

weak expression: see section 14 15

maturing glomerular tuft

weak

regional

weak expression: see section 06 07 08 09 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30232
Entity Detected:	Cmtm4, CKLF-like MARVEL transmembrane domain containing 4 ( MGI:2142888)
Sequence:	sense strand is shown >T30232 CCTCGAGCACCTCCATGATCTCGGGCGCCAGCAGCCCGTACCAACCCACCACCGAGCCGGTGAGCCAGCG CCGCGGGCTGGCCGGCCTGCGCTGCGACCCGGACTACCTGCGCGGCGCGCTCGGCCGCCTCAAGGTCGCC CAAGTGATTCTGGCCTTGATTGCATTTATCTGCATCGAGACTATCATGGAGTGCTCCCCGTGCGAAGGCC TCTACTTCTTTGAGTTCGTAAGCTGCAGTGCATTTGTGGTGACTGGGGTCCTGCTGATTCTCTTCAGCCT CAACCTTCATATGAGGATCCCCCAGATCAACTGGAATCTAACAGATTTGGTCAACACTGGACTCAGCACT TTCTTTTTCTTTATCGCCTCAATCGTGTTGGCTGCTCTAAACCATAAAACCGGAGCGGAAATTGCTGCCG TGATATTTGGCTTCTTGGCAACAGCAGCCTATGCAGTGAGCACATTCCTGGCCATGCAGAAATGGAGAGT CAGCGTCCGCCAGCAGAGCACTAATGACTACATCCGAGCTCGCACCGAGTCGAGAGATGTGGACAGCCGC CCTGAGATCCAGCGCCTGGACACATGAGAACGTCTGGAGTTCTCCCGCTGCCCTCGTGCCCGTCCTTCTA ATCAAGTTTTCTTTCTCTTAGCACGTCAGATTTTTGTGTGAACCAGCTGAATTTTGTCCTGTTTGGTAAA AATTCACTTTGGGAAAGGTTGCCTAGAGTGGCCCAGTGACTGACAGATCTGTGATGGTCACAGATCCAAA GAGGCCCAGGTCCTCTGACCTTGGGTGCCCGTGGGAGCAGCCGACTTTAATGACTGCTCTAGGTCTTAAC TGTCCGTCTCAGAACTCACGGTGTCACCTGACTCTGAGCCACCCTGTCATGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4206772), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 25601. Forward Primer - name:025601_F_IRAV59-62_I02_Cklfsf4, sequence:CCTCGAGCACCTCCATGA; Reverse Primer - name:025601_R_SP6_IRAV59-62_I02_Cklfsf4, sequence:ACACATGACAGGGTGGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15267 same embryo
	EMAGE:15265 same embryo
	EMAGE:15266 same embryo
	EMAGE:15264 same embryo
	EMAGE:15269 same embryo
	EurExpress:euxassay_010370 same experiment
	MGI:4823947 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source