EMAGE mouse gene expression spatial database

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EMAGE:15287

Grik5 glutamate receptor, ionotropic, kainate 5 (gamma 2) ( MGI:95818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010477. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010477_01	euxassay_010477_02	euxassay_010477_03	euxassay_010477_04

euxassay_010477_05	euxassay_010477_06	euxassay_010477_07	euxassay_010477_08	euxassay_010477_09

euxassay_010477_10	euxassay_010477_11	euxassay_010477_12	euxassay_010477_13	euxassay_010477_14

euxassay_010477_15	euxassay_010477_16	euxassay_010477_17	euxassay_010477_18	euxassay_010477_19

euxassay_010477_20	euxassay_010477_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15287_wholemount_strong.wlz

15287_wholemount_moderate.wlz

15287_wholemount_weak.wlz

15287_wholemount_possible.wlz

15287_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15287_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

vibrissa

weak

regional

weak expression: see section 04 05 18 19

hindbrain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 weak expression: see section 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 16 17 18 19 20 weak expression: see section 05 06 15

vagus x ganglion

weak

regional

weak expression: see section 05

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05

trigeminal v nerve

weak

regional

weak expression: see section 07 14

spinal cord

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 11 12 13

neural retina

moderate

regional

moderate expression: see section 01 02 21

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30541
Entity Detected:	Grik5, glutamate receptor, ionotropic, kainate 5 (gamma 2) ( MGI:95818)
Sequence:	sense strand is shown >T30541 CCCCGCCTTCAGTACCTTCGCTTCGCATCTGTCAGCCTGTACCCCAGTAATGAAGATGTCAGCCTGGCAG TCTCCCGAATCCTCAAGTCCTTTAACTACCCCTCAGCTAGCCTCATCTGCGCCAAGGCTGAGTGCCTGCT GCGGCTAGAAGAACTGGTGCGAGGCTTCCTCATCTCCAAGGAGACACTGTCCGTGAGGATGCTTGATGAC AGCCGGGACCCCACGCCGCTACTCAAGGAGATCCGAGATGACAAAGTGTCCACCATCATCATTGATGCCA ATGCGTCCATCTCCCACCTTGTCCTCCGTAAGGCTTCGGAGCTGGGAATGACCTCAGCGTTTTACAAGTA CATCCTCACCACCATGGACTTTCCCATCCTGCATCTGGATGGTATCGTGGAGGACTCCTCCAACATCCTG GGCTTTTCCATGTTCAACACCTCCCACCCCTTCTACCCAGAGTTTGTGCGCAGCCTCAACATGTCCTGGA GGGAGAACTGTGAAGCCAGCACCTATCCTGGCCCTGCGCTGTCCGCAGCCCTGATGTTTGACGCTGTGCA CGTGGTGGTAAGCGCTGTCCGAGAACTGAACCGAAGCCAGGAGATTGGCGTCAAGCCACTGGCCTGCACT TCGGCCAACATTTGGCCCCATGGGACCAGCCTTATGAACTACCTTCGAATGGTAGAGTATGACGGGCTGA CCGGGCGGGTTGAGTTCAACAGCAAAGGGCAGAGGACCAACTACACACTACGCATCCTGGAGAAGTCCCG CCAGGGCCACCGTGAGATAGGGGTGTGGTACTCTAACCGGACCCTGGCCATGAATGCCACCACCCTGGAC ATCAACCTGTCACAGACTCTAGCCAACAAGACTCTGGTGGTCACAACTATCCTGGAGAACCCGTATGTTA TGCGCCGGCCCAACTTCCAGGCCTTGTCAGGGAATGAGCGCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5695215), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59043. Forward Primer - name:059043_F_IRAV111_b03_Grik5, sequence:CCCCGCCTTCAGTACCTT; Reverse Primer - name:059043_R_SP6_IRAV111_b03_Grik5, sequence:GAAGCGCTCATTCCCTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15291 same embryo
	EMAGE:15288 same embryo
	EMAGE:15290 same embryo
	EMAGE:15289 same embryo
	EMAGE:15292 same embryo
	EurExpress:euxassay_010477 same experiment
	MGI:4825231 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source