EMAGE mouse gene expression spatial database

EMAGE:15290

Figf c-fos induced growth factor ( MGI:108037)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010476. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010476_01	euxassay_010476_02	euxassay_010476_03	euxassay_010476_04

euxassay_010476_05	euxassay_010476_06	euxassay_010476_07	euxassay_010476_08	euxassay_010476_09

euxassay_010476_10	euxassay_010476_11	euxassay_010476_12	euxassay_010476_13	euxassay_010476_14

euxassay_010476_15	euxassay_010476_16	euxassay_010476_17	euxassay_010476_18	euxassay_010476_19

euxassay_010476_20	euxassay_010476_21	euxassay_010476_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30537
Entity Detected:	Figf, c-fos induced growth factor ( MGI:108037)
Sequence:	sense strand is shown >T30537 AGAATGGGGAATGGGGAATATCCTCATGATGTTCCATGTGTACTTGGTGCAGGGCTTCAGGAGCGAACAT GGACCAGTGAAGGATTTTTCTTTTGAGCGATCATCCCGGTCCATGTTGGAACGATCTGAACAACAGATCC GAGCAGCTTCTAGTTTGGAGGAGTTGCTGCAAATCGCGCACTCTGAGGACTGGAAGCTGTGGCGATGCCG GTTGAAGCTCAAAAGTCTTGCCAGTATGGACTCACGCTCAGCATCCCATCGCTCCACCAGATTTGCGGCA ACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:864338), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60533. Forward Primer - name:060533_F_IRAV110_h12_Figf, sequence:AGAATGGGGAATGGGGAA; Reverse Primer - name:060533_R_SP6_IRAV110_h12_Figf, sequence:AAGTTGCCGCAAATCTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15291 same embryo
	EMAGE:15288 same embryo
	EMAGE:15287 same embryo
	EMAGE:15289 same embryo
	EMAGE:15292 same embryo
	EurExpress:euxassay_010476 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source