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EMAGE:15297

Plcd1 phospholipase C, delta 1 ( MGI:97614)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297
"Pseudo-wholemount" of euxassay_010490. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010490_01 euxassay_010490_02 euxassay_010490_03 euxassay_010490_04
EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297
euxassay_010490_05 euxassay_010490_06 euxassay_010490_07 euxassay_010490_08 euxassay_010490_09
EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297
euxassay_010490_10 euxassay_010490_11 euxassay_010490_12 euxassay_010490_13 euxassay_010490_14
EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297
euxassay_010490_15 euxassay_010490_16 euxassay_010490_17 euxassay_010490_18 euxassay_010490_19
EMAGE:15297 EMAGE:15297 EMAGE:15297 EMAGE:15297
euxassay_010490_20 euxassay_010490_21 euxassay_010490_22 euxassay_010490_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15297Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15297_wholemount_strong.wlz
15297_wholemount_moderate.wlz
15297_wholemount_weak.wlz
15297_wholemount_possible.wlz
15297_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15297_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 03 04 05 06 19 20 21 22 23 moderate expression: see section 02 07 08 17 18
radius
moderate moderate
regionalmoderate expression: see section 01 weak expression: see section 02
humerus
moderate moderate
regionalmoderate expression: see section 01 02 03 04 22 23 weak expression: see section 21
hindlimb digit 1 metatarsal
weak weak
regionalweak expression: see section 04
hindlimb digit 2 metatarsal
weak weak
regionalweak expression: see section 04
hindlimb digit 3 metatarsal
weak weak
regionalweak expression: see section 04 05 21
hindlimb digit 4 metatarsal
weak weak
regionalweak expression: see section 05 21
hindlimb digit 5 metatarsal
weak weak
regionalweak expression: see section 05 20
tarsus
weak weak
regionalweak expression: see section 04 22
fibula
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 23
tibia
moderate moderate
regionalmoderate expression: see section 01 02 weak expression: see section 23
femur
weak weak
regionalweak expression: see section 03 04 05 20 21 22 23
mandible
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 moderate expression: see section 03
maxilla
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22
palatal shelf
strong strong
regionalstrong expression: see section 09 18 moderate expression: see section 10
bladder
moderate moderate
regionalExpression in the fundus region.
urethra of male
moderate moderate
regionalmoderate expression: see section 12 13
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 03 04 05 06 21 22 23
clavicle
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 15 16 17 weak expression: see section 18
scapula
moderate moderate
regionalmoderate expression: see section 03 04 weak expression: see section 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30285
Entity Detected:Plcd1, phospholipase C, delta 1 ( MGI:97614)
Sequence:sense strand is shown

>T30285
CCAGGACATGAACCAGCCGCTCAGCCACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGAC
CAGCTCACAGGGCCCAGCAGCACGGAGGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGC
TGGACTGCTGGGACGGCCCCAACCAGGAACCCATCATCTACCACGGCTACACTTTTACCTCTAAGATACT
TTTCTGCGACGTGCTCAGGGCCATCCGGGACTACGCCTTCAAGGCATCTCCCTACCCAGTTATCCTGTCC
CTGGAGAACCACTGCAGCCTGGAGCAACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCA
TGCTGTTGGACCAGCCGCTGGATGGGGTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGAT
TCTGTTGAAAGGGAAGAAGCTGGGCGGGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGAT
GTGTCTGACGAAGATGAGGCTGCTGAAATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCA
AGGAGGATAAGCTAAAGCTGGTGCCGGAGCTCTCCGACATGGTCATTTACTGCAAGAGTGTCCACTTTGG
TGGCTTCTCCAGTCCTAGCACCTCTGGGCAGGCATTCTATGAGATGGCTTCCTTCTCCGAGAGCCGTGCC
CTGCGGCTGCTGCAAGAATCAGGAAATAGTTTTGTCCGCCATAACGTGGGCCATCTGAGCAGGATCTACC
CAGCTGGGTGGAGGACAGATTCCTCCAACTACAGTCCCGTGGAGATGTGGAATGGAGGCTGCCAGATTGT
GGCTCTGAACTTCCAAACCCCTGGGCCAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGT
TGTGGGTATGTGCTGAAACCGGCCTTCCTGAGAGACCCTGACACCACCTTCAACTCACGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5068610), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 28120. Forward Primer - name:028120_F_IRAV71-74_O07_Plcd, sequence:CCAGGACATGAACCAGCC; Reverse Primer - name:028120_R_SP6_IRAV71-74_O07_Plcd, sequence:CCCGTGAGTTGAAGGTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15295 same embryo
 EMAGE:15294 same embryo
 EMAGE:15296 same embryo
 EMAGE:15293 same embryo
 EMAGE:15298 same embryo
 EurExpress:euxassay_010490 same experiment
 MGI:4827252 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS