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EMAGE:15308

Polk polymerase (DNA directed), kappa ( MGI:1349767)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308
"Pseudo-wholemount" of euxassay_010517. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010517_01 euxassay_010517_02 euxassay_010517_03 euxassay_010517_04
EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308
euxassay_010517_05 euxassay_010517_06 euxassay_010517_07 euxassay_010517_08 euxassay_010517_09
EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308
euxassay_010517_10 euxassay_010517_11 euxassay_010517_12 euxassay_010517_13 euxassay_010517_14
EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308 EMAGE:15308
euxassay_010517_15 euxassay_010517_16 euxassay_010517_17 euxassay_010517_18 euxassay_010517_19
EMAGE:15308 EMAGE:15308
euxassay_010517_20 euxassay_010517_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15308Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15308_wholemount_strong.wlz
15308_wholemount_moderate.wlz
15308_wholemount_weak.wlz
15308_wholemount_possible.wlz
15308_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15308_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30505
Entity Detected:Polk, polymerase (DNA directed), kappa ( MGI:1349767)
Sequence:sense strand is shown

>T30505
GCGCTGGGTCTAGGTTCAACAGACCTGGCAAGGGATGGAGAAAGGAAAAGCATGAGTGTTGAAAGGACAT
TCAGTGAGATAAGTAAGACAGAGGAACAGTACAGCCTGTGCCAAGAACTGTGCGCTGAGCTCGCCCACGA
CCTCCAGAAGGAAGGACTTAAGGGAAGAACCGTCACCATTAAGCTGAAGAACGTGAATTTTGAAGTAAAA
ACTCGTGCATCTACCGTTCCGGCCGCCATTTCTACTGCAGAGGAAATATTTGCCATTGCCAAGGAGCTGC
TAAGGACAGAAGTTAATGTGGGTTCTCCACACCCCCTGCGGTTAAGACTGATGGGTGTCCGAATGTCTAC
TTTTTCCAGTGAAGATGACAGGAAACACCAACAAAGGAGCATCATTGGTTTCTTACAAGCTGGAAACCAA
GCTTTGTCATCTACTGGGGGTAGTCTAGACAAAACTGCCAAAACTGAGCTTGCAAAGCCCTTAGAAATGT
CTCATAAGAAGAGTTTCTTTGATAAAAAGCGATCAGAAAGAATCTCCAACTGTCAAGACACATCCAGATG
TAAAACTGCGGGTCAGCAAGCTTTACAGATCTTGGAACCATCCCAAGCATTAAAGAAGCTGAGCCAGAGT
TTTGAAACATCAGAGAATTCAAATGACTGTCAGACATTTATATGTCCAGTTTGCTTTAGGGAGCAAGAAG
GTGTCAGTCTGGAAGCCTTTAATGAACATGTAGATGAGTGTCTTGATGGACCGTCAACCAGTGAGAACTC
AAAAATATCCTGTTACTCACATGCTTCCTCTGCAGACATTGGTCAGAAGGAAGATGTACACCCCTCTATT
CCACTGTGTGAGAAACGGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30057338), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58779. Forward Primer - name:058779_F_IRAV108_h04_Polk, sequence:GCGCTGGGTCTAGGTTCA; Reverse Primer - name:058779_R_SP6_IRAV108_h04_Polk, sequence:CCCCCGTTTCTCACACAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15305 same embryo
 EMAGE:15309 same embryo
 EMAGE:15307 same embryo
 EMAGE:15310 same embryo
 EMAGE:15306 same embryo
 EurExpress:euxassay_010517 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS