EMAGE mouse gene expression spatial database

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EMAGE:15309

Rassf2 Ras association (RalGDS/AF-6) domain family member 2 ( MGI:2442060)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010507. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010507_01	euxassay_010507_02	euxassay_010507_03	euxassay_010507_04

euxassay_010507_05	euxassay_010507_06	euxassay_010507_07	euxassay_010507_08	euxassay_010507_09

euxassay_010507_10	euxassay_010507_11	euxassay_010507_12	euxassay_010507_13	euxassay_010507_14

euxassay_010507_15	euxassay_010507_16	euxassay_010507_17	euxassay_010507_18	euxassay_010507_19

euxassay_010507_20	euxassay_010507_21	euxassay_010507_22	euxassay_010507_23	euxassay_010507_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15309_wholemount_strong.wlz

15309_wholemount_moderate.wlz

15309_wholemount_weak.wlz

15309_wholemount_possible.wlz

15309_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15309_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 12

hindbrain meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21

pons ventricular layer

moderate

regional

moderate expression: see section 12

midbrain meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

moderate

regional

moderate expression: see section 07 08

spinal cord ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12

spinal cord meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15

heart valve

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30546
Entity Detected:	Rassf2, Ras association (RalGDS/AF-6) domain family member 2 ( MGI:2442060)
Sequence:	sense strand is shown >T30546 ACCAGCGGTGAAAAGCAGAGGCTGAAGAGCTCCGACTACCCTCTGATCGCCCGCATCCTCCAGGGCCCTT GTGAACAGATCTCCAAAGTTTTCCTGATGGAGAAGGACCAGGTGGAGGAGGTCACCTATGACGTGGCCCA GTATATAAAGTTCGAGATGCCAGTCCTTAAAAGCTTCATTCAGAAACTCCAGGAGGAGGAAGACCGGGAG GTGGAGAAGCTGATGCGCAAGTACACTGTGCTCCGGCTAATGATTCGGCAAAGGCTCGAGGAGATAGCAG AGACCCCCGAGACTATCTGAGCCACAGGAATGAAGACAGTCCAGACACTCCAGGGGCCGAGGCTGACCTG AGAAGACCCACTGGAAGCTAGGCATCCTACAGAAACGTTTTCACACGATCCTTGCAGCCTTCCAAAAGCC AGCACTGCTCCCTACAGTAGGACACAGAAGCAGCAGACAGCTCCCCACCTTTGGACCCCTGATCTTCTTT GTCTCATTCTCAAGGACTTTTGTCCTAAGGAGGACCCACAATGTGTATGCTTGCATACCTGAGTGTGTGT ATGTGTATGTGCACGCATACGCAAGTGTGCATATGTGTACTTACCATGTCCACGTGCCTCTCTGATGTTC TTACATTTGGTTAAATTCCCAGCAGCCAGCAGGTGTCACAAGGCAGGAGGGCGCACGTGCTGCTCCTGTG TGGGAAGAACAGGGAAGTGACTGAGGCTTTTGGCTTCCTCCAGTGCCAACGGCAGCCGCACGGATCTGCC ACGCTTGACAGGGAAGAGGAGGCAGCAGGCTCACCGTTTGGAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5698333), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59103. Forward Primer - name:059103_F_IRAV111_d12_Rassf2, sequence:ACCAGCGGTGAAAAGCAG; Reverse Primer - name:059103_R_SP6_IRAV111_d12_Rassf2, sequence:CATTCCAAACGGTGAGCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15305 same embryo
	EMAGE:15307 same embryo
	EMAGE:15308 same embryo
	EMAGE:15310 same embryo
	EMAGE:15306 same embryo
	EurExpress:euxassay_010507 same experiment
	MGI:4827633 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source