EMAGE mouse gene expression spatial database

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EMAGE:15393

Dpp4 dipeptidylpeptidase 4 ( MGI:94919)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010550. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010550_01	euxassay_010550_02	euxassay_010550_03	euxassay_010550_04

euxassay_010550_05	euxassay_010550_06	euxassay_010550_07	euxassay_010550_08	euxassay_010550_09

euxassay_010550_10	euxassay_010550_11	euxassay_010550_12	euxassay_010550_13	euxassay_010550_14

euxassay_010550_15	euxassay_010550_16	euxassay_010550_17	euxassay_010550_18	euxassay_010550_19

euxassay_010550_20	euxassay_010550_21	euxassay_010550_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15393_wholemount_strong.wlz

15393_wholemount_moderate.wlz

15393_wholemount_weak.wlz

15393_wholemount_possible.wlz

15393_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15393_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pericardium

moderate

regional

moderate expression: see section 04 05 06 10 11 12 13 14 15 16 17 weak expression: see section 07 08 09

peritoneal component

moderate

regional

moderate expression: see section 02 03 04 05

pleural cavity

moderate

regional

moderate expression: see section 21

pleura

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 07

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

cochlea

moderate

regional

moderate expression: see section 08 16

utricle

moderate

regional

moderate expression: see section 05 06 07 17 18 19 20

midgut

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

urinary system

moderate

spotted

moderate expression: see section 05 06 20

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

right lung

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30827
Entity Detected:	Dpp4, dipeptidylpeptidase 4 ( MGI:94919)
Sequence:	sense strand is shown >T30827 GGCTGGGTCGGAAGATTTAGGCCCGCAGAACCTCACTTCACCTCTGATGGAAGCAGCTTCTATAAGATCA TCAGCGACAAAGATGGCTATAAACACATCTGCCACTTCCCGAAAGATAAGAAAGACTGTACATTTATTAC AAAAGGAGCCTGGGAAGTCATTAGTATCGAAGCTCTGACCAGCGATTATCTATACTACATTAGTAACCAA TATAAAGAAATGCCAGGAGGAAGAAATCTCTATAAAATTCAACTTACTGACCACACAAATGTGAAGTGCC TTAGTTGTGACCTGAATCCAGAAAGATGTCAGTATTATGCAGTATCATTTAGTAAAGAGGCAAAGTACTA TCAGCTGGGATGTTGGGGCCCCGGTCTGCCCCTCTACACTCTACATCGTAGCACGGATCATAAAGAGCTG CGAGTCCTGGAAGACAATTCTGCTTTGGATAGAATGCTGCAGGATGTCCAGATGCCTTCAAAAAAATTGG ACTTCATTGTTTTGAATGAAACAAGATTTTGGTATCAAATGATCTTGCCCCCTCATTTTGATAAATCCAA GAAATATCCTCTACTATTAGATGTATATGCAGGTCCCTGTAGTCAAAAAGCAGATGCTTCCTTCAGACTG AACTGGGCCACTTACCTTGCAAGTACAGAAAACATCATAGTAGCTAGCTTTGACGGCAGAGGAAGTGGTT ACCAAGGAGATAAGATCATGCATGCAATCAACAGAAGATTGGGAACACTGGAAGTTGAAGATCAAATTGA AGCAGCGAGGCAATTTGTAAAAATGGGATTTGTGGACAGCAAGCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3982742), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17380. Forward Primer - name:017380_F_IRAV20-23_B13_Dpp4, sequence:GGCTGGGTCGGAAGATTT; Reverse Primer - name:017380_R_SP6_IRAV20-23_B13_Dpp4, sequence:TCGCTTGCTGTCCACAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15388 same embryo
	EMAGE:15390 same embryo
	EMAGE:15389 same embryo
	EMAGE:15391 same embryo
	EMAGE:15392 same embryo
	EurExpress:euxassay_010550 same experiment
	MGI:4824381 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source