EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15398

Pdia5 protein disulfide isomerase associated 5 ( MGI:1919849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010598. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010598_01	euxassay_010598_02	euxassay_010598_03	euxassay_010598_04

euxassay_010598_05	euxassay_010598_06	euxassay_010598_07	euxassay_010598_08	euxassay_010598_09

euxassay_010598_10	euxassay_010598_11	euxassay_010598_12	euxassay_010598_13	euxassay_010598_14

euxassay_010598_15	euxassay_010598_16	euxassay_010598_17	euxassay_010598_18	euxassay_010598_19

euxassay_010598_20	euxassay_010598_21	euxassay_010598_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15398_wholemount_strong.wlz

15398_wholemount_moderate.wlz

15398_wholemount_weak.wlz

15398_wholemount_possible.wlz

15398_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15398_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 17 weak expression: see section 04 16 18 19

mandible

moderate

regional

moderate expression: see section 05 06 07 09 10 11 15 16 17 18 19 20 21 weak expression: see section 03 04

maxilla

moderate

regional

moderate expression: see section 06 07 09 10 11 17 18 19

orbito-sphenoid

moderate

regional

moderate expression: see section 03 04 05 06 weak expression: see section 01 02 20 21 22

clavicle

moderate

regional

moderate expression: see section 06 07 weak expression: see section 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30804
Entity Detected:	Pdia5, protein disulfide isomerase associated 5 ( MGI:1919849)
Sequence:	sense strand is shown >T30804 CGCAATAACGTGCTGGTGCTTTACTCGGAATCCGAGGTAGCAGCTGAAAGTCATCTTAAGCTCCTGTCCA CCGTGGCCCAGGCAGTGAAAGGCCAAGGCACCGTCTGCTGGGTGGACTGCGGTGACGCAGAGAGTAGAAA ATTGTGCAAGAAGATGAAAGTTGACCTCAGCCCAAAGGACAAAAAGATTGAATTATTCCATTACCAGGAC GGCGCCTTTCACATGCAGTATGACCGAGCTGTGACGTTGAAGTCCATAGTGGCCTTTCTGAAAGACCCTA AAGGTCCCCCACTGTGGGAAGAAGATCCTGGAGCCAAAGATGTTGTGCACATCGACAGTGAGAAGGACTT CAGACGACTCCTGAAGAGAGAAGAGAAGCCACTGCTCATGATGTTCTATGCTCCCTGGTGCAGCATGTGC AAGAGAATCATGCCACATTTCCAGAAGGCTGCCACCCAAGTGCGAGGCCACATTGTCCTGGCTGGGATGA ACGTCTACCCATCAGAGTTCGAGAACATCAAGGAAGAGTACAATGTCCGCGGCTACCCTACCATCTGCTA TTTCGAGAAGGGGCGGTTTCTGTTCCCGTATGAGAACTATGGGTCCACGGCCGAGGACATCGTGGAGTGG TTGAAGAATCCACTGCCACCACAGCCCCAGGTCCCCGAGACTCCCTGGGCAGATGAGGGCGGCTCCGTTT ATCACCTGACCGATGAAGACTTTGACCAGTTTGTGAAGGAGCACTCCTCTGTCCTCGTCATGTTCCACGC CCCATGGTGTGGACATTGTAAGAAAATGAAGCCAGAGTTTGAGAGTGCTGCTGAAGTCCTCCACGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3600849), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16320. Forward Primer - name:016320_F_IRAV16-19_J02_Pdir, sequence:CGCAATAACGTGCTGGTG; Reverse Primer - name:016320_R_SP6_IRAV16-19_J02_Pdir, sequence:TCCGTGGAGGACTTCAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15394 same embryo
	EMAGE:15399 same embryo
	EMAGE:15397 same embryo
	EMAGE:15395 same embryo
	EMAGE:15396 same embryo
	EurExpress:euxassay_010598 same experiment
	MGI:4827131 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source