EMAGE mouse gene expression spatial database

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EMAGE:15443

Fam110a family with sequence similarity 110, member A ( MGI:1921097)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010641. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010641_01	euxassay_010641_02	euxassay_010641_03	euxassay_010641_04

euxassay_010641_05	euxassay_010641_06	euxassay_010641_07	euxassay_010641_08	euxassay_010641_09

euxassay_010641_10	euxassay_010641_11	euxassay_010641_12	euxassay_010641_13	euxassay_010641_14

euxassay_010641_15	euxassay_010641_16	euxassay_010641_17	euxassay_010641_18	euxassay_010641_19

euxassay_010641_20	euxassay_010641_21	euxassay_010641_22	euxassay_010641_23	euxassay_010641_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15443_wholemount_strong.wlz

15443_wholemount_moderate.wlz

15443_wholemount_weak.wlz

15443_wholemount_possible.wlz

15443_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15443_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

weak

regional

weak expression: see section 11 12 13 16 17 18 20

not examined

regional

not examined expression: see section 13

midbrain mantle layer

moderate

regional

moderate expression: see section 08 11 12 16 weak expression: see section 07 09 10 13 14 15 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31192
Entity Detected:	Fam110a, family with sequence similarity 110, member A ( MGI:1921097)
Sequence:	sense strand is shown >T31192 GAGTGCTGTGGAACGCCTGGAGGCTGATAAGGCTAAGTACGTCAAGAGCCTGCGTGTGGCCAACACCCGC CAAGAGCCGGTGCAGCCCCCGCTGGTCAGACAGCCCCTCTTCAGCCCTGGTCCTCGCGGCCCAGTGCTCA CACCCAGCCGCCGAGCCCTGCCCTGTTCTGGCCGCCGGCCCCAACTGGACCTCGACATCCTTAGCAGCCT CATCAACTTGTGTGATAGTCCTGTGTCCCCATCTGAGGCCAGCCGAACACCTGGAAGGCCAGAAGGATCT GCCCACAAGGTCCCACCAGCCACCCCTCCTCGTCCGCCACCCAGTACTGTCGCGGTGCGCCGAGTGGACG TTCGTCCCTTGCCTGCTTCACCTGCTCGGCCCTATCCATCACCTGGCACCACTACCACTTCCAGCCCCGG CCGGCCCCCTGGCCTGCAGCGGTCCAAGTCGGACTTGAGTGAGCGCTTTTCCAGGGCAGCAGCTGATCTT GAGCGCTTTTTTTAACTTCTGTGGCCTGGACCCAGAGGAAGCGCGAGGATTGGGAGTGGCCCACTTAGCA AGGGCCAGCTCGGACATCGTGTCTCTAGCCGGGCCAAGTGCTGGACCTTGCAGCTCTGAAGGGGGCTGCT CACGCCGCAGCTCTGCTACAGTGGAAGAGCGGGCCCTGGATCGTGTCCCCTATGGGGTGTCTGTGATCGA GCGAAACGCTCGTGTGATCAAGTGGCTATATGGGTTGCGGCAGGCTCGTGACCCTCCCACCACTGAGGGT TAGGCCTCCTGGACTGGGGTTTCCCGGGAAGACATCGTGAAGAGACAGTTGGCACTTTTTCCTTTCCTGT ATCCATTGGCTGCCTTCCTGCAGACACAGAGACTCTTGGGTGAATTTAATGTAGAGGCCAAGACTTAGAC CAGCATCCTCTGGCAAGCAAGGACTGACCCACAGAAGTTTGCTGTTGCCCTTGGCCTACCCCACCCCCTA ACAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3987755), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 55204. Forward Primer - name:055204_F_IRAV47_d01_5430432M24Rik, sequence:GAGTGCTGTGGAACGCCT; Reverse Primer - name:055204_R_SP6_IRAV47_d01_5430432M24Rik, sequence:TTTGTTAGGGGGTGGGGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15445 same embryo
	EMAGE:15444 same embryo
	EMAGE:15442 same embryo
	EMAGE:15441 same embryo
	EMAGE:15440 same embryo
	EurExpress:euxassay_010641 same experiment
	MGI:4824689 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source