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EMAGE:15516

Zfp597 zinc finger protein 597 ( MGI:1918313)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516
"Pseudo-wholemount" of euxassay_010711. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010711_01 euxassay_010711_02 euxassay_010711_03 euxassay_010711_04
EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516
euxassay_010711_05 euxassay_010711_06 euxassay_010711_07 euxassay_010711_08 euxassay_010711_09
EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516
euxassay_010711_10 euxassay_010711_11 euxassay_010711_12 euxassay_010711_13 euxassay_010711_14
EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516
euxassay_010711_15 euxassay_010711_16 euxassay_010711_17 euxassay_010711_18 euxassay_010711_19
EMAGE:15516 EMAGE:15516 EMAGE:15516 EMAGE:15516
euxassay_010711_20 euxassay_010711_21 euxassay_010711_22 euxassay_010711_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15516Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15516_wholemount_strong.wlz
15516_wholemount_moderate.wlz
15516_wholemount_weak.wlz
15516_wholemount_possible.wlz
15516_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15516_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38121
Entity Detected:Zfp597, zinc finger protein 597 ( MGI:1918313)
Sequence:sense strand is shown

>T38121
ACCTGAGGACACATAAGCGAATCCACACTGGTGAGAAGCCTTACAAGTGTACCGAGTGTGCTGCCAGCTT
CCGACAGCAGTCACACCTGTCTAGGCACATGAATAGCCATTTAAAGGAGAAACTATATACATGTAGTGTA
TGTGGGAGAGGTTTTATGTGGCTCCCAGGATTGGCAGAACATCAGAAGAGTCATACTGATAAGAAATCTT
ATGAATGTGCTGACCACAATCGGGAAGCAAATCTGGCTTTGCCTGAAGAGAGAGGTTCATCAGACACACT
GCCCCAGCACACACACTGTGTGAAGACCTTGGAGCAGCCCTCAGACCCTACTCTCCCTGAGAAAGACCAT
AAGGAGGACTCGAAAAACTGCAGTATTGATGATGAGGACTTTTTTTCATTCTCCAGATTCAAACCCTTAC
AGTGTCTTGATTGTGACATGACTTTTCCTTGTTTCTCGGAGCTTGTCTCTCACCAAACCATTCATGACCT
GGAAAAACCTCATAAGTGCAAAACATGTACAAAAACTTTTGCTTTCGAGTCAGAGCTTGTATCCCATGAG
AAGAGCCACAGAAGAGAAGAGCCTTTTAAATGCACAGTGTGTGGGAAGAGTTTCAGTGTGAGTATGCAGC
TCGTCACTCATAAACGAGCCCATAGGAGAAACAGCAAGTAAATACAAGCCTTTGCTCGAATTGGGCTCTT
CAGTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 103852. Forward Primer - name:103852_F_cDNA_Zfp597, sequence:ACCTGAGGACACATAAGCGAAT; Reverse Primer - name:103852_N_SP6_cDNA_Zfp597, sequence:ACACTGAAGAGCCCAATTCGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15517 same embryo
 EMAGE:15514 same embryo
 EMAGE:15513 same embryo
 EMAGE:15515 same embryo
 EMAGE:15512 same embryo
 EurExpress:euxassay_010711 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS