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EMAGE:15563

Slc25a16 solute carrier family 25 (mitochondrial carrier, Graves disease autoantigen), member 16 ( MGI:1920382)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563
"Pseudo-wholemount" of euxassay_010736. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010736_01 euxassay_010736_02 euxassay_010736_03 euxassay_010736_04
EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563
euxassay_010736_05 euxassay_010736_06 euxassay_010736_07 euxassay_010736_08 euxassay_010736_09
EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563
euxassay_010736_10 euxassay_010736_11 euxassay_010736_12 euxassay_010736_13 euxassay_010736_14
EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563 EMAGE:15563
euxassay_010736_15 euxassay_010736_16 euxassay_010736_17 euxassay_010736_18 euxassay_010736_19
EMAGE:15563 EMAGE:15563 EMAGE:15563
euxassay_010736_20 euxassay_010736_21 euxassay_010736_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15563Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15563_wholemount_strong.wlz
15563_wholemount_moderate.wlz
15563_wholemount_weak.wlz
15563_wholemount_possible.wlz
15563_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15563_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38320
Entity Detected:Slc25a16, solute carrier family 25 (mitochondrial carrier, Graves disease autoantigen), member 16 ( MGI:1920382)
Sequence:sense strand is shown

>T38320
AGAGGCAATCCTCTTGTCTCTGCCTCCCACATACTGAAATTCCAGGCATGGGTCACCATGCCTGCTCCAA
CATATTTATTAATGGCAAACGTTATACCTTAGCACCTTGAAAAGCCATCTTGGGTAAAGTGTTATTTGGA
AGGAAAAGAAGGTTCGCATTTGAGTTCAAGAGATTGCCCTTAGTCCTACCATACTTTCCAAACTCTGCAT
TGTAATTGTGGTTACTGAGGGGGAGGGGCTGGAATCGAACCCAGGGCCTTTGCATGCCAAGCACATGTAC
CCACTAGGCCACATCCCCAGCCTGCCAGCGCCATGCCACTGAGCTATGTCACTGGCCTCTTCCTTTCTTA
GGATGGTGCTGGGGATCACATTCATGCATGCTGATTGTGTAATCGCACACTGCCAATGGTCCACACCCCA
GCCCGCTAACCCCGCTCGTTATTTCCCCTCCCACTCTGTCCCTTCTCTACTTGTTCTGGGGAGTAAACTC
AGGGTCTCTCTGCCTATCTTGCTAGATAAGCGCTCTACCTCTGAACTACATCTCCAGCCTTCTAACATTG
TGTAATGTTATTTAAATCAGCTGACTTCCTTTTATTGTAGCAGAATGAGAACTGAAAATGTAAGGTCTGT
GAAACAGAGCCAGCAACAAAGTAGTGTTCTTATTATGTTTCTTCTCAGAATTCTCTCCAGTGGGTAAAAA
GACACCTTTCCGTCTAGTGGTTTGAGAAGCACTTCCTTCCTTGCGCTCTTTCTTTCTAACTCTCTTTGGG
GATTGAGTCCAGGACGTTGTACATGGCAGGCATGATGAGATCCCATCAGAAAGTCAGCTGTACACTGAGG
TATATCCCCAGTTGTCCTTCCTTCTCAATA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 147798. Forward Primer - name:147798_F_cDNA_Slc25a16, sequence:AGAGGCAATCCTCTTGTCTCTG; Reverse Primer - name:147798_N_SP6_cDNA_Slc25a16, sequence:TATTGAGAAGGAAGGACAACTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15560 same embryo
 EMAGE:15559 same embryo
 EMAGE:15562 same embryo
 EMAGE:15558 same embryo
 EMAGE:15561 same embryo
 EurExpress:euxassay_010736 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS