Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15713

Kcna3 potassium voltage-gated channel, shaker-related subfamily, member 3 ( MGI:96660)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713
"Pseudo-wholemount" of euxassay_010872. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010872_01 euxassay_010872_02 euxassay_010872_03 euxassay_010872_04
EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713
euxassay_010872_05 euxassay_010872_06 euxassay_010872_07 euxassay_010872_08 euxassay_010872_09
EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713
euxassay_010872_10 euxassay_010872_11 euxassay_010872_12 euxassay_010872_13 euxassay_010872_14
EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713
euxassay_010872_15 euxassay_010872_16 euxassay_010872_17 euxassay_010872_18 euxassay_010872_19
EMAGE:15713 EMAGE:15713 EMAGE:15713 EMAGE:15713
euxassay_010872_20 euxassay_010872_21 euxassay_010872_22 euxassay_010872_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15713Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15713_wholemount_strong.wlz
15713_wholemount_moderate.wlz
15713_wholemount_weak.wlz
15713_wholemount_possible.wlz
15713_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15713_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
olfactory cortex mantle layer
weak weak
regionalweak expression: see section 12 13 14 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38443
Entity Detected:Kcna3, potassium voltage-gated channel, shaker-related subfamily, member 3 ( MGI:96660)
Sequence:sense strand is shown

>T38443
CTTCGACGCCATCCTCTACTACTACCAGTCCGGGGGCCGCATTCGCCGGCCGGTCAACGTGCCCATCGAC
ATCTTCTCCGAGGAGATCCGCTTTTACCAGCTGGGTGAGGAGGCCATGGAAAAGTTCCGTGAGGATGAGG
GCTTCCTGCGGGAGGAGGAGCGACCCCTGCCCCGCCGTGACTTCCAGCGCCAGGTGTGGCTGCTCTTCGA
ATATCCGGAGAGCTCCGGGCCGGCCCGGGGCATTGCCATTGTGTCAGTGCTGGTCATTCTCATCTCCATT
GTCATCTTCTGCTTGGAGACGCTTCCCGAGTTTCGCGATGAGAAAGACTATCCCGCCTCCCCGTCGCAGG
ACGTGTTTGAGGCTGCCAACAACAGCACGTCGGGGGCCCCTTCTGGAGCCTCCAGCTTCTCGGACCCCTT
CTTCGTGGTGGAGACCTTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150606. Forward Primer - name:150606_F_cDNA_Kcna3, sequence:CTTCGACGCCATCCTCTACTAC; Reverse Primer - name:150606_N_SP6_cDNA_Kcna3, sequence:ACAAGGTCTCCACCACGAAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15714 same embryo
 EMAGE:15717 same embryo
 EMAGE:15716 same embryo
 EMAGE:15715 same embryo
 EMAGE:15712 same embryo
 EurExpress:euxassay_010872 same experiment
 MGI:4825696 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS