EMAGE mouse gene expression spatial database

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EMAGE:15715

Pou4f3 POU domain, class 4, transcription factor 3 ( MGI:102523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010878. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010878_01	euxassay_010878_02	euxassay_010878_03	euxassay_010878_04

euxassay_010878_05	euxassay_010878_06	euxassay_010878_07	euxassay_010878_08	euxassay_010878_09

euxassay_010878_10	euxassay_010878_11	euxassay_010878_12	euxassay_010878_13	euxassay_010878_14

euxassay_010878_15	euxassay_010878_16	euxassay_010878_17	euxassay_010878_18	euxassay_010878_19

euxassay_010878_20	euxassay_010878_21	euxassay_010878_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15715_wholemount_strong.wlz

15715_wholemount_moderate.wlz

15715_wholemount_weak.wlz

15715_wholemount_possible.wlz

15715_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15715_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 12

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 10 11 12 15 16 weak expression: see section 09 13 14

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 16 17 18 19 21

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 10 11 12 14 15

neural retina

weak

regional

weak expression: see section 02 03 04 05 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38447
Entity Detected:	Pou4f3, POU domain, class 4, transcription factor 3 ( MGI:102523)
Sequence:	sense strand is shown >T38447 CGACTTACTTGAGCACATCTCGCCCACGCTGAGCGTGAGTGGCCTAGGGGCCCCGGAGCACTCGGTGATG CCGGCGCAGATCCACCCGCATCATCTAGGCGCCATGGGCCACTTGCATCAGGCCATGGGCATGAGTCACC CGCATGCCGTAGCACCGCACAGTGCCATGCCCGCGTGTCTCAGCGATGTGGAGTCAGACCCTCGAGAGCT GGAAGCGTTCGCCGAGCGCTTCAAGCAGAGGCGCATCAAGTTGGGGGTCACCCAGGCGGACGTGGGCGCG GCTTTAGCCAATCTTAAGATCCCCGGTGTGGGCTCGCTCAGCCAGAGCACCATCTGCAGGTTCGAGTCTC TTACTCTGTCGCACAACAACATGATCGCTCTCAAGCCGGTCCTCCAGGCCTGGCTGGAGGAGGCCGAGGC CGCCTACCGAGAGAAGAACAGCAAGCCAGAGCTCTTCAACGGCAGTGAGCGTAAGCGCAAACGCACGTCC ATCGCCGCGCCAGAGAAGCGCTCACTCGAAGCCTATTTCGCCATCCAGCCACGTCCTTCATCCGAGAAGA TCGCGGCCATCGCGGAGAAACTGGACCTTAAAAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 150166. Forward Primer - name:150166_F_cDNA_Pou4f3, sequence:CGACTTACTTGAGCACATCTCG; Reverse Primer - name:150166_N_SP6_cDNA_Pou4f3, sequence:CTTTTTAAGGTCCAGTTTCTCCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15714 same embryo
	EMAGE:15717 same embryo
	EMAGE:15713 same embryo
	EMAGE:15716 same embryo
	EMAGE:15712 same embryo
	EurExpress:euxassay_010878 same experiment
	MGI:4827342 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source