EMAGE mouse gene expression spatial database

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EMAGE:15716

Myh3 myosin, heavy polypeptide 3, skeletal muscle, embryonic ( MGI:1339709)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010874. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010874_01	euxassay_010874_02	euxassay_010874_03	euxassay_010874_04

euxassay_010874_05	euxassay_010874_06	euxassay_010874_07	euxassay_010874_08	euxassay_010874_09

euxassay_010874_10	euxassay_010874_11	euxassay_010874_12	euxassay_010874_13	euxassay_010874_14

euxassay_010874_15	euxassay_010874_16	euxassay_010874_17	euxassay_010874_18	euxassay_010874_19

euxassay_010874_20	euxassay_010874_21	euxassay_010874_22	euxassay_010874_23	euxassay_010874_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15716_wholemount_strong.wlz

15716_wholemount_moderate.wlz

15716_wholemount_weak.wlz

15716_wholemount_possible.wlz

15716_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15716_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 17 18 19 20 21 22 23 24

upper leg muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 21 22 23 24 not examined expression: see section 20

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03

hand

strong

regional

strong expression: see section 03 04 05 06 07 08 24

foot

strong

regional

strong expression: see section 11 12 13

not examined

regional

not examined expression: see section 20

lower leg rest of mesenchyme

strong

regional

strong expression: see section 04 05 06 07 08

upper leg rest of mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

heart atrium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

heart ventricle

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17

tail paraxial mesenchyme

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38446
Entity Detected:	Myh3, myosin, heavy polypeptide 3, skeletal muscle, embryonic ( MGI:1339709)
Sequence:	sense strand is shown >T38446 GGCTCAAGAAGAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGATGAGCAGACCTTGAGCCT CCAGCTTCAGAAGAAAATCAAGGAGCTACAGGCTCGCATCGAGGAGCTGGAGGAAGAGATAGAGGCAGAG AGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGCTGGAGGAGCTGAGTGAGC GGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAAGCGTGAGGCTGAGTTCTT GAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTGGCCACGCTGCGGAAGAAG CACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGA AGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGAGAGCGTGTCCAAATCCAA G
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 149776. Forward Primer - name:149776_F_cDNA_Myh3, sequence:GGCTCAAGAAGAAGGACTTTGA; Reverse Primer - name:149776_N_SP6_cDNA_Myh3, sequence:CTTGGATTTGGACACGCTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15714 same embryo
	EMAGE:15717 same embryo
	EMAGE:15713 same embryo
	EMAGE:15715 same embryo
	EMAGE:15712 same embryo
	EurExpress:euxassay_010874 same experiment
	MGI:4826542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source