EMAGE mouse gene expression spatial database

EMAGE:15784

Eif5b eukaryotic translation initiation factor 5B ( MGI:2441772)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013163. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013163_01	euxassay_013163_02	euxassay_013163_03	euxassay_013163_04

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38893
Entity Detected:	Eif5b, eukaryotic translation initiation factor 5B ( MGI:2441772)
Sequence:	sense strand is shown >T38893 GTTGGAACATGATCCCCAGTATGCAGTAATTTTGGCCTTTGATGTAAGAATTGAACGGGACGCACAAGAG ATGGCTGATAGTTTGGGAGTTAGAATCTTTAGTGCAGAAATTATTTATCACTTATTTGATGCCTTTACAA AATACAGACAGGACTACAAAAAACAAAAACAAGAAGAATTTAAGCACATAGCAGTATTTCCCTGCAAGAT GAAAATCCTCCCTCAATACATTTTCAATTCTCGAGATCCAATAGTAATAGGGGTCACTGTGGAGGCTGGT CAAGTGAAACAAGGAACACCTATGTGCGTTCCAAGCAAAAATTTTGTTGACATTGGGATAGTAACAAGCA TTGAAATAAACCACAAGCAGGTGGATGTTGCAAAAAAAGGACAAGAGGTCTGTGTCAAAATAGAACCTAT CCCTGGGGAATCTCCCAAAATGTTTGGGAGACATTTTGAAGCTACAGATATACTTGTCAGCAAGATCAGC CG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 177806. Forward Primer - name:177806_F_cDNA_Eif5b, sequence:GTTGGAACATGATCCCCAGTAT; Reverse Primer - name:177806_N_SP6_cDNA_Eif5b, sequence:CGGCTGATCTTGCTGACAAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15782 same embryo
	EMAGE:15781 same embryo
	EMAGE:15780 same embryo
	EMAGE:15779 same embryo
	EMAGE:15783 same embryo
	EurExpress:euxassay_013163 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source