EMAGE mouse gene expression spatial database

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EMAGE:15813

Kif1b kinesin family member 1B ( MGI:108426)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013179. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013179_01	euxassay_013179_02	euxassay_013179_03	euxassay_013179_04

euxassay_013179_05	euxassay_013179_06	euxassay_013179_07	euxassay_013179_08	euxassay_013179_09

euxassay_013179_10	euxassay_013179_11	euxassay_013179_12	euxassay_013179_13	euxassay_013179_14

euxassay_013179_15	euxassay_013179_16	euxassay_013179_17	euxassay_013179_18	euxassay_013179_19

euxassay_013179_20	euxassay_013179_21	euxassay_013179_22	euxassay_013179_23	euxassay_013179_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15813_wholemount_strong.wlz

15813_wholemount_moderate.wlz

15813_wholemount_weak.wlz

15813_wholemount_possible.wlz

15813_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15813_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 20 21 22 23 24

upper leg muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 24

hand

strong

regional

strong expression: see section 02 03 04 05 20 21 22

foot

strong

regional

strong expression: see section 07 08 18 19 20

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata floor plate

strong

regional

strong expression: see section 12

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 09 10 11 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13

metencephalon floor plate

strong

regional

strong expression: see section 11 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 04 05 06 07 09 10 11 12 16 17 18 19 20

pons ventricular layer

strong

regional

strong expression: see section 06 07 17 18 moderate expression: see section 08 09 10 13 14 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

spinal cord ventricular layer

strong

regional

strong expression: see section 12 13 14 15

eye skeletal muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 20 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16

tail paraxial mesenchyme

strong

regional

strong expression: see section 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39056
Entity Detected:	Kif1b, kinesin family member 1B ( MGI:108426)
Sequence:	sense strand is shown >T39056 AGTGGAGAACTTGGCAAAGAAGAGCGCGTTTCCCAGCTGATGAACGGGGATCCAGCTTTTAGACGTGGAC GTCTACGTTGGATGAGGCAAGAACAAATTCGGTTTAAGAACCTGCAACAGCAAGAGATAACAAAGCAGCT TCGTCGGCAGAACGTACCCCACAGGTTCATCCCTCCTGAGAACCGGAAGCCCCGCTTTCCCTTTAAGAGC AATCCTAAACACAGAAACTCTTGGAGTCCTGGGACACATATTATTATAACAGAAGATGAGGTTATTGAGC TAAGAATTCCAAAAGATGAAGAAGGAAGAAAAGAAAATAAAGAAGAAAGCCAAGAAAAAGTGGGCAGAGC AGCTTCTAGAGATGTACAGTCAGCGTGGGGCACAAGAAGTCAGGATCATATCCAAGTTAGCAAACAGCAC ATCAGCAACCAACAGCCACCACCTCAGCTACGGTGGAGAAGCAATTCTCTCAATAACGGCCAACCGAAAA CCACGCGCTGCCAGGCGACTGCCTCCTCGGAGTCCCTAAACTCCCACAGCGGTCACCCCACCGCTGATCT ACAAACTTTCCAGGCCAAGCGTCACATTCACCAACACCGGCAGCCTTACTGTAATTATAACACTGGAGGT CAGGTAGAGGGTAGCACAGCCAGCTGCTGTCAAAAGCAGACTGACAAGCCCAGCCACTGTAACCAGTTCG TGACACCTCCGAGGATGAGGAGACAATTCTCAGCACCCAATCTCAAAGCAGGTCGAGAAACCACAGTCTA GGTAGATTACTGGACAAACTTGAAGTTGTAGCAATGGAACCAGGCAGTGTTATGTTAGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96700. Forward Primer - name:096700_F_cDNA_Kif1b, sequence:AGTGGAGAACTTGGCAAAGAAG; Reverse Primer - name:096700_N_SP6_cDNA_Kif1b, sequence:AGCTAACATAACACTGCCTGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15810 same embryo
	EMAGE:15812 same embryo
	EMAGE:15809 same embryo
	EMAGE:15814 same embryo
	EMAGE:15811 same embryo
	EurExpress:euxassay_013179 same experiment
	MGI:4825754 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source