EMAGE mouse gene expression spatial database

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EMAGE:15920

Evi2a ecotropic viral integration site 2a ( MGI:95458)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013368. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013368_01	euxassay_013368_02	euxassay_013368_03	euxassay_013368_04

euxassay_013368_05	euxassay_013368_06	euxassay_013368_07	euxassay_013368_08	euxassay_013368_09

euxassay_013368_10	euxassay_013368_11	euxassay_013368_12	euxassay_013368_13	euxassay_013368_14

euxassay_013368_15	euxassay_013368_16	euxassay_013368_17	euxassay_013368_18	euxassay_013368_19

euxassay_013368_20	euxassay_013368_21	euxassay_013368_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15920_wholemount_strong.wlz

15920_wholemount_moderate.wlz

15920_wholemount_weak.wlz

15920_wholemount_possible.wlz

15920_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15920_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8284
Entity Detected:	Evi2a, ecotropic viral integration site 2a ( MGI:95458)
Sequence:	sense strand is shown >T8284 CCGTGACATGTAGTTGAGTTGCAGCGGAAAGGAAATGTGTCATCTGTGGTTTGGTTTTAAGAGTGGAAAG CTAGCTGTACATATCCTCTTTAAAAAACCCTGCAGATTTAAGTTTCACAGTCTCCAAAGTATTCTACTTA AAATAAGAAGAGCAGAACTGAGGTGGCTTACGAAGTGACGGCTGGGCGAGCTCTGAAGATAGCAGTGGAC CTGTAGGTATGGAGCACAAAGGACAGTACCTGCATCTTGTTTTCCTGATGACAACAGTGTGGGCCTCGTC GTCTTCTGGAACAAGACCAAACTACACACACCTGTGGGCTAGCAGTGTCACTGCCTCAGGTTCCAGTAAT CAGAATGGCTCCAGCAGACATCCGAGTGACAATAACACAAACCTTGTCACTCCTGCCGTGGGTCACAAGG TGAGCGCCACAGACAAGCCTGCATCATCTCCCCCTGTGCCTTTAGCTTCCACATCTACACTCAAGTCCTC CACGCCCCATGCCTTCAGAAACAGTTCTCCAACAGCAGAGATCAAAAGTCAGGGGGAAACGTTTAAAAAG GAAGTCTGTGAGGAGAACACCAGCAACACGGCCATGCTAATTTGCTTAATTGTAATTGCAGTGCTTTTCC TTATCTGTACCTTTCTATTTCTATCAACTGTGGTTCTGGCAAACAAAGTCTCATCTCTCAAAAGGTCAAA GCAAGTAGGCAAGCGCCAGCCGCGGAGCAACGGTGACTTTCTGGCAAGTAGTGGGCTCTGGACTGCTGAG TCGGACACTTGGAAAAGAGCAAAGGAGCTCACAGGCTCCAACCTCCTATTGCAATCTCCTGGTGTGCTTA CAGCAGCCAGGGAGAGAAAGCACGAAGAAGGAACTGAAAAACTCAACTAGGCTGTGGGTTGGAAAGGGAA ATGCAAAGCAGCAATGAGGAAGGCAGAGTTAGAAATAAAGCTGGCTGGTGGTATTTCCCATCCACGTTTG CTCAGATGATCCAAAGTCTGACATGCTCTTTGTTTAGCGTCAGACCAGAAAGGGAGATGTATGCTTGCTC AAGGTTTATATCTGTTTCGTACTTCATATTTTTAAGAAAGTAAAACAACAGCAGCTAAAGAAACTAATAG AAATAACATGAGGTAATTAAACATTCCTGTTCAGCAGTGAATGACCTCTGTCTGAATGTACTTGAACTTG GAAGAGTTCTTTTAAGCCCTTAGAGCCAAGCAGATGTCAAATTCCCAATGTGTAGAATTCAACTATGTAA CGCAATGGTACATTCTCACTGGATGAGATAGAACCTGTACCAGTTGCTATCTTAGTACAGCTAATATTAG GCTACATCTTTTTACCTTTACATATGGCGTAAGACAAGACTGCTTTTCTATTATACATAACACATTAAAA CTTAATTTTGTACAATAAATGAAAACAGACTTTAGAACAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1396641 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1396641 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15921 same embryo
	EMAGE:15919 same embryo
	EMAGE:15922 same embryo
	EMAGE:15923 same embryo
	EMAGE:15918 same embryo
	EurExpress:euxassay_013368 same experiment
	MGI:4824637 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source