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EMAGE:15921

Dip2b DIP2 disco-interacting protein 2 homolog B (Drosophila) ( MGI:2145977)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921
"Pseudo-wholemount" of euxassay_013366. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013366_01 euxassay_013366_02 euxassay_013366_03 euxassay_013366_04
EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921
euxassay_013366_05 euxassay_013366_06 euxassay_013366_07 euxassay_013366_08 euxassay_013366_09
EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921
euxassay_013366_10 euxassay_013366_11 euxassay_013366_12 euxassay_013366_13 euxassay_013366_14
EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921 EMAGE:15921
euxassay_013366_15 euxassay_013366_16 euxassay_013366_17 euxassay_013366_18 euxassay_013366_19
EMAGE:15921 EMAGE:15921 EMAGE:15921
euxassay_013366_20 euxassay_013366_21 euxassay_013366_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15921Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15921_wholemount_strong.wlz
15921_wholemount_moderate.wlz
15921_wholemount_weak.wlz
15921_wholemount_possible.wlz
15921_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15921_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39048
Entity Detected:Dip2b, DIP2 disco-interacting protein 2 homolog B (Drosophila) ( MGI:2145977)
Sequence:sense strand is shown

>T39048
AGTCATGGAGCTGTGCACTAAAGGGCTGGGGAACCAGGTGGAGGTGCTGAAGACCAGAGGGATCAATCTT
TCTTGCATCCGGACCTGTGTGGTGGTGGCAGAGGAGCGGCCCCGTGTCAGCCTTCAGCAGTCCTTCTCCA
AGCTCTTCAAAGACATCGGTCTGTCCCCTCGAGCTGTCAGCACCACTTTTGGATCCAGAGTCAATGTAGC
AATATGTTTACAGGGAACCTCAGGGCCGGATCCAACAACTGTGTATGTGGATCTGAAGTCGCTGAGACAT
GACAGGGTCCGTCTTGTGGAACGGGGTGCCCCCCAGAGCCTCCTGCTTTCTGAATCTGGAAAGATCTTAC
CTGGAGTAAAAGTAGTTATTGTCAATCCTGAGACCAAAGGACCTGTTGGAGACTCTCACCTGGGAGAGAT
CTGGGTGAATAGCCCCCATACCGCCAGTGGCTACTATACCATCTACGACAGCGAGACTCTTCAAGCTGAT
CATTTCAATACCCGTCTGAGCTTTGGAGATGCTGCCCAGACACTCTGGGCCCGGACAGGATACCTCGGGT
TTGTCCGCAGGACTGAGCTCACTGCAGCCACTGGAGAACGCCATGACGCCTTGTATGTGGTGGGTGCGCT
GGATGAGACACTGGAGCTGAGGGGATT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67569. Forward Primer - name:067569_F_cDNA_AI317237, sequence:AGTCATGGAGCTGTGCACTAAA; Reverse Primer - name:067569_N_SP6_cDNA_AI317237, sequence:AATCCCCTCAGCTCCAGTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15919 same embryo
 EMAGE:15920 same embryo
 EMAGE:15922 same embryo
 EMAGE:15923 same embryo
 EMAGE:15918 same embryo
 EurExpress:euxassay_013366 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS