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EMAGE:15922

Eid2b EP300 interacting inhibitor of differentiation 2B ( MGI:1924095)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922
"Pseudo-wholemount" of euxassay_013369. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013369_01 euxassay_013369_02 euxassay_013369_03 euxassay_013369_04
EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922
euxassay_013369_05 euxassay_013369_06 euxassay_013369_07 euxassay_013369_08 euxassay_013369_09
EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922
euxassay_013369_10 euxassay_013369_11 euxassay_013369_12 euxassay_013369_13 euxassay_013369_14
EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922
euxassay_013369_15 euxassay_013369_16 euxassay_013369_17 euxassay_013369_18 euxassay_013369_19
EMAGE:15922 EMAGE:15922 EMAGE:15922 EMAGE:15922
euxassay_013369_20 euxassay_013369_21 euxassay_013369_22 euxassay_013369_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15922Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15922_wholemount_strong.wlz
15922_wholemount_moderate.wlz
15922_wholemount_weak.wlz
15922_wholemount_possible.wlz
15922_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15922_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40038
Entity Detected:Eid2b, EP300 interacting inhibitor of differentiation 2B ( MGI:1924095)
Sequence:sense strand is shown

>T40038
TTCACCTATAACCTCCGAGGAAGGAGGCCGGAGGACCGGACTTCAAGGTCATCCTCCGCCATGTAGCGAG
TTGGAGGCCAGCCTGGGCTACAGGAGACTGTCTTTTAAAAATAAAAAATAAAAATAAAAGATTGGTGGCC
AGGAAGTCAGGTGACTCCTAGAATCAAGGGCAAAGGCAGATGTCTGGGCAAATGTCATGAGGTGGGGAAT
CCATGCCAGAATCCAGCCGTGGGCAGGGTGGAATGAAGCAGGCCGGGAGAGAGAAGGGCACTTGAGGCTT
GAGGCTGACAAGCAAAGAGGACTCTTCTGACTTCCACCTTGTGACTGTCAGCAATCTTTCCCTCCCCTGC
ATTTGTTGAAAATCATGGTTGCTTCACTGCGAAGGACGATGGTTTTGCAAAGATGAAGTCTAAGAAATAT
CAATGAAAAAGACCATAGAAATCAGCGGAGATGCGGGAAGAAACCAGGGGATGTGCTTGCTAGATTCTGT
TCCTCCCGGGTTTTACTGAGTAAGAAGAAAGCTGTAAACCACTTGGACATTTCAGAATTGAGGGTCGTCC
CATCGTCAGCTGACAAGATCCATGTATCGTTACTTCTTGTGTTTGATGTTCTGTAGACGAGTTGTAAATA
TTCAGATAGGGAAAACCATGTATGTGTTGTGAGATGAATATTTTTCCAGACCTTGTGTAGTTGTAACCAT
GCGGAAATAGATGAGTGATTATTTAAATAATACAGACACAAGACTCAAATTCTGCAAAAGATACTATGGG
AAGTTAAACTTATCTGCCAAATTGAAAATAGGTATCCATACAGATTTGTGTTTTCTGTAGGGTTTTTCAA
ACAGATTTTATAAGGCATATATGAACTGGTACCAGTCTAGTTATCCCCCAATCTTGGGTGCTGAGGATGG
ACCCCAAGCCTTCTTGCCCTGCTCCCATTCCTTCTGAATCAAGGTAGGGTA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108377. Forward Primer - name:108377_F_cDNA_LOC434156, sequence:TTCACCTATAACCTCCGAGGAA; Reverse Primer - name:108377_N_SP6_cDNA_LOC434156, sequence:TACCCTACCTTGATTCAGAAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15921 same embryo
 EMAGE:15919 same embryo
 EMAGE:15920 same embryo
 EMAGE:15923 same embryo
 EMAGE:15918 same embryo
 EurExpress:euxassay_013369 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS