EMAGE mouse gene expression spatial database

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EMAGE:15963

Aak1 AP2 associated kinase 1 ( MGI:1098687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013375. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013375_01	euxassay_013375_02	euxassay_013375_03	euxassay_013375_04

euxassay_013375_05	euxassay_013375_06	euxassay_013375_07	euxassay_013375_08	euxassay_013375_09

euxassay_013375_10	euxassay_013375_11	euxassay_013375_12	euxassay_013375_13	euxassay_013375_14

euxassay_013375_15	euxassay_013375_16	euxassay_013375_17	euxassay_013375_18	euxassay_013375_19

euxassay_013375_20	euxassay_013375_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15963_wholemount_strong.wlz

15963_wholemount_moderate.wlz

15963_wholemount_weak.wlz

15963_wholemount_possible.wlz

15963_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15963_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 03 04 05 16 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18

vagus x ganglion

weak

regional

weak expression: see section 05 06 15

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 04 05 06 16

dorsal root ganglion

weak

regional

weak expression: see section 04 05 06 08 09 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40029
Entity Detected:	Aak1, AP2 associated kinase 1 ( MGI:1098687)
Sequence:	sense strand is shown >T40029 AGCTTCTATGCTCTTGCCATTCCTCCAGCTTTCTGCAGCATTTACTCACAGGGTTAGAATGAGCAGTTAC TAACCTTATTGCTACTTGCTGGTTGCCTGATAAAAGCCCACATCATTAAAAGCTTGGAGATAGATTTTCC TTCTGTAATATTAAGTACACACAACCTTACCTATTGCAGTTAAACAGAATCCAGAGATTTCTATTATTTC AATCTGTATAATCAGGGATTTGTTGTTAAAATATGGGCAAATACTATATGCATTTTTTTAATCTTCTCTC TGATGAGTGGAAGTAATTACTGTACCAGAAAGTATCACAAATTGGTATTTTCCCCCTAGAAAACCAAACT TAATTTCTAAAGTTTCTAAAAATATGGTCCTCAAAGAAGATATTTTTAAATGATCACCTCTAAGTAGTAG TATTATAATTGTTACAATGATTTCTGAAGTAGACATCAGAAATGTTTCATGTGGTTGAATGTTCCCTCCA GAAGCATCTGGTTTTGCTTGGGAAGTGAATGGTGACCCTAGACAGGCCAGTGGCATGTGAGTTGTTTAAA CTCTTGTTCTTAGACATAACATCACCAGATATGATAGCAAGGATGTTGTTGCATTTCCCCAGTAGCCTCG CTCACAGCATATTCGATCCTGTTTTCCCCACACCTCTGCCTTTGAGTAATATTGAACTATTCCAGAAACC ACTGGGTGAGATGAAGAGCAGTTACTGCTCAAGACACAGAAAGCCACAGGCAATTTCTAAAGAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108097. Forward Primer - name:108097_F_cDNA_LOC434058, sequence:AGCTTCTATGCTCTTGCCATTC; Reverse Primer - name:108097_N_SP6_cDNA_LOC434058, sequence:CTTCTTTAGAAATTGCCTGTGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15962 same embryo
	EMAGE:15961 same embryo
	EMAGE:15960 same embryo
	EMAGE:15959 same embryo
	EMAGE:15964 same embryo
	EurExpress:euxassay_013375 same experiment
	MGI:4822855 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source