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EMAGE:16095

Krt2 keratin 2 ( MGI:96699)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095
"Pseudo-wholemount" of euxassay_013552. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013552_01 euxassay_013552_02 euxassay_013552_03 euxassay_013552_04
EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095
euxassay_013552_05 euxassay_013552_06 euxassay_013552_07 euxassay_013552_08 euxassay_013552_09
EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095
euxassay_013552_10 euxassay_013552_11 euxassay_013552_12 euxassay_013552_13 euxassay_013552_14
EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095
euxassay_013552_15 euxassay_013552_16 euxassay_013552_17 euxassay_013552_18 euxassay_013552_19
EMAGE:16095 EMAGE:16095 EMAGE:16095 EMAGE:16095
euxassay_013552_20 euxassay_013552_21 euxassay_013552_22 euxassay_013552_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16095Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16095_wholemount_strong.wlz
16095_wholemount_moderate.wlz
16095_wholemount_weak.wlz
16095_wholemount_possible.wlz
16095_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16095_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 19 20 21
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 17 18
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 07 17
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 17 18 19
trigeminal v nerve
strong strong
regionalstrong expression: see section 17
spinal cord
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 09 15 16
cervical ganglion
strong strong
regionalstrong expression: see section 07 08 16 17
thoracic ganglion
strong strong
regionalstrong expression: see section 11 13
dorsal root ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 15 16 17
neural retina
strong strong
regionalstrong expression: see section 02 03 04 22 23
stomach
strong strong
spottedstrong expression: see section 03 04 05 06 07 08 09 10
midgut
strong strong
spottedstrong expression: see section 11 12 13 17 18 moderate expression: see section 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45118
Entity Detected:Krt2, keratin 2 ( MGI:96699)
Sequence:sense strand is shown

>T45118
AAAGTTCTGGAGGAAGAGGGTCTTATGGAGGACGAGGAGGAGGAGGAGGAGGAGGAAGCTCCTATGGCTC
TGGAGGCAGAAGCTCTGGCTCCAGAGGATCTGGATCCGGATCTGGAGGAGGAGGATACAGCTCTGGAGGA
GGTTCCAGGGGAGGATCTGGAGGAGGATATGGCTCTGGAGGAGGCTCCAGGGGAGGATCTGGAGGAGGAT
ACGGCTCTGGAGGTGGCTCCGGCTCTGGAGGAGGATACAGCTCTGGAGGAGGCTCCAGGGGAGGATCTGG
AGGAGGAGGAGCCAGCTCTGGAGGAGGCTCTAGAGGAGGCTCCAGCTCTGGAGGAGGCTCCAGAGGTGGC
TCCAGCTCTGGTGGGGGAGGATATAGTTCTGGAGGAGGCTCCAGAGGTGGCTCCAGCTCAGGAGGAGCAG
GATCTAGCTCTGAAAAGGGAGGTTCTGGGTCAGGGGAAGGTTGTGGCTCAGGTGTGACCTTCTCCTTTAG
ATAGAGATATGTCTTTTCTCTCTACTCTTGGATTCCAGAATGTCAGCTGTTATCTCTGTATCTCTAACTC
TGATTAACCCCAATATTTGTAATCCACCTGATGTGATTTTTTGTTTTTGAGATGGGGGGTGATAAAAAGA
TGTCTCTTAGCAATTAGAGCCAGAGATACTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 212812. Forward Primer - name:212812_F_exon_Krt2-17, sequence:AAAGTTCTGGAGGAAGAGGGTC; Reverse Primer - name:212812_N_SP6_exon_Krt2-17, sequence:CAGTATCTCTGGCTCTAATTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16091 same embryo
 EMAGE:16092 same embryo
 EMAGE:16094 same embryo
 EMAGE:16097 same embryo
 EMAGE:16093 same embryo
 EMAGE:16096 same embryo
 EurExpress:euxassay_013552 same experiment
 MGI:4825832 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS