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EMAGE:16190

Rps9 ribosomal protein S9 ( MGI:1924096)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190
euxassay_017055_01 euxassay_017055_02 euxassay_017055_03 euxassay_017055_04 euxassay_017055_05
EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190
euxassay_017055_06 euxassay_017055_07 euxassay_017055_08 euxassay_017055_09 euxassay_017055_10
EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190
euxassay_017055_11 euxassay_017055_12 euxassay_017055_13 euxassay_017055_14 euxassay_017055_15
EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190
euxassay_017055_16 euxassay_017055_17 euxassay_017055_18 euxassay_017055_19 euxassay_017055_20
EMAGE:16190 EMAGE:16190 EMAGE:16190 EMAGE:16190
euxassay_017055_21 euxassay_017055_22 euxassay_017055_23 euxassay_017055_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16190Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16190_wholemount_strong.wlz
16190_wholemount_moderate.wlz
16190_wholemount_weak.wlz
16190_wholemount_possible.wlz
16190_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16190_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6115
Entity Detected:Rps9, ribosomal protein S9 ( MGI:1924096)
Sequence:sense strand is shown

>T6115
GCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCAACATGCCGGTCGCCAGAAGCTGGGTTTGTCGCA
AAACCTATGTGACCCCACGGAGACCCTTCGAGAAGTCGCGTCTCGACCAGGAGCTAAAGTTGATTGGAGA
GTATGGACTCCGGAACAAACGTGAGGTTTGGAGGGTCAAGTTTACCCTGGCCAAGATCCGTAAGGCGGCC
CGGGAGCTGTTGACGCTAGACGAGAAGGATCCCCGGCGTCTGTTTGAAGGCAATGCTCTCCTGCGGCGGC
TTGTTCGCATTGGGGTGCTGGACGAGGGCAAGATGAAGCTGGATTACATCCTGGGCCTGAAGATTGAGGA
TTTCTTGGAGAGGCGGCTGCAGACCCAGGTCTTTAAGCTGGGCCTGGCCAAATCTATTCACCATGCCCGT
GTGCTCATCCGCCAGCGTCACATTAGGGTCCGCAAGCAGGTGGTGAACATCCCATCCTTCATTGTTCGCC
TGGACTCTCAGAAGCCCATCGACTTCTCCCTCCGTTTTCCTTATGGCGGCGGCCGTCCAGGCCGAGTGAA
GAGGAAGAATGCCAAGAAAGGCCAGGGCGGGGCTGGAGCTGGTGATGATGAGGAAGAGGATTAATTAATA
CTTGGCTGAACTGGAGGATTGTTTAGTTTTCCAGCTGAAAAATAAAAAAGAATTGATACTTGGTTAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3494314 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3494314 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16191 same embryo
 EMAGE:16187 same embryo
 EMAGE:16188 same embryo
 EMAGE:16192 same embryo
 EMAGE:16189 same embryo
 EurExpress:euxassay_017055 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS