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EMAGE:16262

Fam123c family with sequence similarity 123, member C ( MGI:3026939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262
"Pseudo-wholemount" of euxassay_017140. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017140_01 euxassay_017140_02 euxassay_017140_03 euxassay_017140_04
EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262
euxassay_017140_05 euxassay_017140_06 euxassay_017140_07 euxassay_017140_08 euxassay_017140_09
EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262
euxassay_017140_10 euxassay_017140_11 euxassay_017140_12 euxassay_017140_13 euxassay_017140_14
EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262
euxassay_017140_15 euxassay_017140_16 euxassay_017140_17 euxassay_017140_18 euxassay_017140_19
EMAGE:16262 EMAGE:16262 EMAGE:16262 EMAGE:16262
euxassay_017140_20 euxassay_017140_21 euxassay_017140_22 euxassay_017140_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16262Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16262_wholemount_strong.wlz
16262_wholemount_moderate.wlz
16262_wholemount_weak.wlz
16262_wholemount_possible.wlz
16262_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16262_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63371
Entity Detected:Fam123c, family with sequence similarity 123, member C ( MGI:3026939)
Sequence:sense strand is shown

>T63371
CTCATGAGGCTGAGTTCACAAGGACCCGCAGGTTTCATCCAAGTCTACTCTTTACCCAGGAAAGGAGAAC
AAGATACACCGCAGTGTCACAGTGCTTCTGTTTGGGAATGGCAAGGACCAGTCCTATCTTAGCAGGAGCC
TGGGGCCTCCAGCATGGTATACGTGCACAGCTTTGCCAGGGCTGACCAGACCCTGCCTGACCAATAGGTT
CTCTCCTAGTGATTCTGTGCACTTCCTTCAGCTTGTTCTCTGAGGCCTTGGGTGGGAGGATACCCACTGT
TCTCCCCACCTCTGCCATTCTGCCTTGGAGTTGCAAAGGCCTTATCTTCTGCCCTTTCCACTCCCAGCGT
GGCCATCTCGCCTCCAGGGAGCTCTCTGAATGAAGCTGTGCTCACCACCCCAACCCAAAAGCAACACACG
CACCGAACCCAGACATCCAGAAACAGGAAAGGACCTGCTGGCTGCTATCCCCAGGAAGGGTAGATATCAT
TTTTGACCCAGCCTGAGGGCAGGGACAGAGGGTCCATCCTCCTTGTCAAGGATTTGATTCTACCTCAGCA
AAAAATAACACAGTGGCAGGCAGAGGATAAGGACTTGACAGGTAGCTGTAAAACTTGTCACCCGGACCAT
ATGTAGGCAGGTTCCTGGAGAGGCCACACTTCATACAGAGCTCCTGTGGAGAGGTACAGGAACTACAGGT
AAGCACTGAAGTCCCATTGATATCAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 73277. Forward Primer - name:073277_F_cDNA_9430069J07Rik, sequence:CTCATGAGGCTGAGTTCACAAG; Reverse Primer - name:073277_N_SP6_cDNA_9430069J07Rik, sequence:GTGATATCAATGGGACTTCAGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16265 same embryo
 EMAGE:16266 same embryo
 EMAGE:16261 same embryo
 EMAGE:16264 same embryo
 EMAGE:16263 same embryo
 EurExpress:euxassay_017140 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS