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EMAGE:16289

Polr3b polymerase (RNA) III (DNA directed) polypeptide B ( MGI:1917678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289
"Pseudo-wholemount" of euxassay_017132. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017132_01 euxassay_017132_02 euxassay_017132_03 euxassay_017132_04
EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289
euxassay_017132_05 euxassay_017132_06 euxassay_017132_07 euxassay_017132_08 euxassay_017132_09
EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289
euxassay_017132_10 euxassay_017132_11 euxassay_017132_12 euxassay_017132_13 euxassay_017132_14
EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289 EMAGE:16289
euxassay_017132_15 euxassay_017132_16 euxassay_017132_17 euxassay_017132_18 euxassay_017132_19
EMAGE:16289
euxassay_017132_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16289Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16289_wholemount_strong.wlz
16289_wholemount_moderate.wlz
16289_wholemount_weak.wlz
16289_wholemount_possible.wlz
16289_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16289_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63409
Entity Detected:Polr3b, polymerase (RNA) III (DNA directed) polypeptide B ( MGI:1917678)
Sequence:sense strand is shown

>T63409
GCTATGGAGCGAGTATGCTTTTGCTGGAGAGACTAATGATCTCAAGTGATGCCTTTGAGGTTGACGTCTG
CGGGCAGTGTGGACTCCTGGGATATTCTGGCTGGTGCCACTACTGCAAATCTTCGTGCCACGTGTCCTCC
CTCCGCATCCCGTATGCCTGCAAGCTGCTCTTCCAGGAGTTACAGTCCATGAACATCATCCCCAGGCTGA
AACTGGCCAAGTACAACGAGTAAGGCTGTGCGGTGCTCACGAGGGTGCAGTCGGCCGACGGCCTGTACAG
CCTGAGGCAGGGGGCTCTCGGTGACTTCTGTGCTCATTCTGCTTCACGGAGGAGGCAGAGGAGGAGGAGG
AAGAAGAGGAAGAAGGAGAGGAGGAGGAAGAGAATGAAGGAGAGGAGGAAGAGGAGCAGCAGCAGCAGCT
GTCTGAGGTCCAGAGGCTGGCTTGACACCCTTGATCTGGAGCCTGAAGCCATAGGAGGTGCTGGGCCCCG
CACAGCACTGAGTGGAGTCACACACAGATGGACACAAGCTAGAGAGAGTACCAACGCCTATTAATTTTGT
TGTTTGTTTGTTTGTGTTGAGTGGGAGTCTCACTCTATCCCAGGTGGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91366. Forward Primer - name:091366_F_cDNA_Polr3b, sequence:GCTATGGAGCGAGTATGCTTTT; Reverse Primer - name:091366_N_SP6_cDNA_Polr3b, sequence:TCCCACCTGGGATAGAGTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16285 same embryo
 EMAGE:16284 same embryo
 EMAGE:16286 same embryo
 EMAGE:16288 same embryo
 EMAGE:16287 same embryo
 EurExpress:euxassay_017132 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS