EMAGE mouse gene expression spatial database

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EMAGE:16375

Shkbp1 Sh3kbp1 binding protein 1 ( MGI:2385803)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017189_01	euxassay_017189_02	euxassay_017189_03	euxassay_017189_04

euxassay_017189_05	euxassay_017189_06	euxassay_017189_07	euxassay_017189_08	euxassay_017189_09

euxassay_017189_10	euxassay_017189_11	euxassay_017189_12	euxassay_017189_13	euxassay_017189_14

euxassay_017189_15	euxassay_017189_16	euxassay_017189_17	euxassay_017189_18	euxassay_017189_19

euxassay_017189_20	euxassay_017189_21	euxassay_017189_22	euxassay_017189_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16375_wholemount_strong.wlz

16375_wholemount_moderate.wlz

16375_wholemount_weak.wlz

16375_wholemount_possible.wlz

16375_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16375_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7841
Entity Detected:	Shkbp1, Sh3kbp1 binding protein 1 ( MGI:2385803)
Sequence:	sense strand is shown >T7841 GGGCTATGGCCGTCGCGACGACTGCAGTGGAGGGGGTCCCGAGTCGGGGAGCACCCGGGGAAGTGATTCA TCTGAACGTAGGGGGCAAGCGGTTCAGTACCTCTCGCCAGACGCTTACCTGGATCCCAGACTCCTTCTTT TCCAGTCTTTTGAGCGGACGCATCTCAACCCTGAAAGATGAGACCGGAGCTATCTTCATCGACCGGGACC CCACCGTCTTTGCCCCCATCCTTAATTTCCTGCGGACCAAAGAGTTAGATCCCAGGGGTGTCCATGGTTC CAGCCTCCTCCATGAAGCCCAATTCTACGGACTCACTCCACTGGTTCGTCGCCTGCAGGTGAGGGAAGAG TTGGATCGATCTTCCTGCGGAAATGTCCTCTTCAATGGTTATCTACCTCCTCCAGTGTTCCCAGTGAAGC GGCGAAACCGGCACAGCCTGGTGGGGCCCCAGCAAATAGGGGGACGCCCAGCCCCTGTTCGACGGAGCAA CACGATGCCCCCCAACTTGGGTAATGCAGGGCTGCTGGGCCGAATGTTAGATGAGAGAGCACCTCCATCC CCATCAGGCCAACCAGAGGAGCCAGGGATGGTGCGCCTGGTGTGTGGTCACCACAACTGGATTGCTGTGG CATATACCCACTTTCTCGTCTGTTATAGGTTGAAGGAAGCCTCGGGCTGGCAGCTTGCATTCTCCAGCCC GCGCCTGGACTGGCCCATCGAGCGCCTGGCTCTTGCGGCTCGGGTGCTTGGCGGTGCTCCGGGTGAGCAT GACAAGATGGTGGCTGCAGCTACTGGCAGCGAGATCCTTCTGTGGGCCCTACAAGCACAAGGTGGTGGCT CTGAGATAGGTGTCTTCCACCTGGGTGTGCCAGTGGAGGCCTTGTTCTTTGTTGGGAATCAGCTCATTGC CACAAGTCACACAGGGCGCATCGGTGTCTGGAACGCTGTGACCAAGCACTGGCAGGTCCAGGAGGTCCAG CCTATCACGAGCTATGATGCAGCCGGCTCCTTCCTCCTCCTGGGCTGCAGCAATGGCTCCATCTATTATG TAGATGTGCAGAAATTCCCCCTGCGGATGAAGGACAACGATCTCCTTGTCAGTGAGCTCTACCGAGACCC AGCAGAGGATGGGGTCACCGCCCTCAGCGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATT GAGATTGCCTATGGCACCAGCTCCGGGGTGGTGCGGGTCATTGTGCAGCACCCTGAGACGGTGGGCTCGG GACCCCAGCTCTTCCAGACCTTCAGTGTGCACCGCAGCCCGGTCACTAAGATCATGCTGTCAGAGAAGCA TCTTATCTCTGTGTGTGCCGACAACAACCATGTTCGGACGTGGTCTGTGACCCGCTTTCGAGGCATGATC TCCACCCAGCCTGGTTCCACTCCGCTGGCATCCTTCAAGATCCTGGCGCTGGAATCTGTTGATGGGCTCG GTGGCTGCAGTGCTGGCAATGACATTGGTCCCTATGGTGAACGGGATGACCAGCAGGTGTTCATCCAGAA AGTGGTGCCAAATGCCAGTCAGCTATTTGTGCGTCTCTCTTCCACTGGGCAGCGGGTGTGTTCTGTGCGC TCGGTGGATGGGTCTCCCACGACTGCCTTCACAGTGTTGGAGTGTGAGGGCTCCAGGCGGCTGGGCTCCA GACCCCGACGTTACCTGCTCACTGGTCAGGCCAACGGCAGCCTGGCCATGTGGGACCTGACCACTGCCAT GGATGGCCTGGGCCAAACTCCTGCAGGCGGCCTGACAGAAGAAGAGCTGATGGACCAACTGGAGCAATGT GAGCTGAGCCCATTGGCTTCCTCTCGGGGCTCTTTCCCCAGCCCCTCACCCAGGACCTCTCTTACCAGTC TCCACTCAGCCTCCAGCAACACCTCCCTGTATGGCCCCAGAGGGAGCCCCAGCCCACCACAGGCAGAGGC CCGTCGCCGCGGGGCAGGAAGCTTCGTGGATCGGTGTCAGGAACTGGCAAGGGGTGCGCCAGAGCTCAGA TGGCCGCCAACACCAGCCCCTCGACCCTCCACCAGTCTTGGGAATCCCCTGACAGCTCTCAAGAAAACTC TTAACGAAACTTCCTTTTGAATCTAGCCACCAATGTGCCTTCATGTGGACTGGTCCCAGGGGACTTGGAA CTGGGGAGGGGGCTTCCTGTTGGAGCCAGGGCCAGGGGCTCCTTGGTGTAATCTGTGTCGCCAGGTTCCC ACCTCTCTCCTTTTCTAAAAGCCAAAGCCACTATCTCCAATAAAACCGTCACTGCCAACAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6336438 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6336438 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16379 same embryo
	EMAGE:16376 same embryo
	EMAGE:16378 same embryo
	EMAGE:16377 same embryo
	EMAGE:16374 same embryo
	EurExpress:euxassay_017189 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source