EMAGE mouse gene expression spatial database

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EMAGE:16379

Mcts2 malignant T cell amplified sequence 2 ( MGI:1913655)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017180. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017180_01	euxassay_017180_02	euxassay_017180_03	euxassay_017180_04

euxassay_017180_05	euxassay_017180_06	euxassay_017180_07	euxassay_017180_08	euxassay_017180_09

euxassay_017180_10	euxassay_017180_11	euxassay_017180_12	euxassay_017180_13	euxassay_017180_14

euxassay_017180_15	euxassay_017180_16	euxassay_017180_17	euxassay_017180_18	euxassay_017180_19

euxassay_017180_20	euxassay_017180_21	euxassay_017180_22	euxassay_017180_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16379_wholemount_strong.wlz

16379_wholemount_moderate.wlz

16379_wholemount_weak.wlz

16379_wholemount_possible.wlz

16379_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16379_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8312
Entity Detected:	Mcts2, malignant T cell amplified sequence 2 ( MGI:1913655)
Sequence:	sense strand is shown >T8312 CGACAACGGCCAGCTCAGATGCCGGTGCCATCCTTCCTGTAGACCGCGACTCGGACGGTCGGCGATCCAC GCCTCGCCTCATCTCTCATACCTGACGGTGCCCCTTCACCAAGATCATGTTCAAGAAATTTGACGAGAAG GAAAGTGTGTCCAACTGCATCCAACTGAAAACTTCCGTTATTAAGGGTATTAAGAGCCAACTGACTGAGC AGTTTCCAGGTATCGAGCCGTGGCTTAATCAAATCATGCCTAAGAAAGATCCCGTCAAAATAGTGAGATG CCATGAACACATGGAAATCCTTACAGTCAACGGAGAATTACTGTTTTTCAGGCAGAGAAAAGGACCTTTT TATCCAACGCTAAGACTACTTCACAAATACCCGTTTATCCTGCCACACCAGCAGGTCGACAAAGGAGCCA TCAAATTTGTGCTCAGTGGTGCAAATATCATGTGCCCGGGTTTAACGTCTCCTGGAGCGAAGCTCTACAC TGCTGCAGTAGATACCATCGTGGCGGTCATGGCAGAGGGGAAAGAGCATGCCCTGTGTGTCGGAGTCATG AAGATGGCTGCAGCAGACATTGAGAAAATCAACAAGGGGATCGGCATTGAGAATATCCATTATCTAAATG ACGGGCTGTGGCACATGAAGACATATAAGTGAGCCTTAGACACAATACACCTGAGCTAAATATAAATATT GTGTTGTATCTCTGTGTGTGTCTGTATGTGACATCATGAAGACAATACCTCTATGGTTATGCTGAATAAA TTCAACAAATACCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:1478068 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1478068 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16376 same embryo
	EMAGE:16378 same embryo
	EMAGE:16375 same embryo
	EMAGE:16377 same embryo
	EMAGE:16374 same embryo
	EurExpress:euxassay_017180 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source