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EMAGE:16828

Slc25a10 solute carrier family 25 (mitochondrial carrier, dicarboxylate transporter), member 10 ( MGI:1353497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828
"Pseudo-wholemount" of euxassay_008673. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008673_01 euxassay_008673_02 euxassay_008673_03 euxassay_008673_04
EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828
euxassay_008673_05 euxassay_008673_06 euxassay_008673_07 euxassay_008673_08 euxassay_008673_09
EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828
euxassay_008673_10 euxassay_008673_11 euxassay_008673_12 euxassay_008673_13 euxassay_008673_14
EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828 EMAGE:16828
euxassay_008673_15 euxassay_008673_16 euxassay_008673_17 euxassay_008673_18 euxassay_008673_19
EMAGE:16828 EMAGE:16828 EMAGE:16828
euxassay_008673_20 euxassay_008673_21 euxassay_008673_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16828Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16828_wholemount_strong.wlz
16828_wholemount_moderate.wlz
16828_wholemount_weak.wlz
16828_wholemount_possible.wlz
16828_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16828_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1237
Entity Detected:Slc25a10, solute carrier family 25 (mitochondrial carrier, dicarboxylate transporter), member 10 ( MGI:1353497)
Sequence:sense strand is shown

>T1237
CCTCGAGNCTGTTGGCCTACTGGAGTTGTGCTCACCGGGGTCGGGCCAGGTCGCTGCTGCTCTGGCCATG
GCCGAGGCACGCGCATCTCGCTGGTACTTTGGGGGGCTGGCTTCCTGCGGAGCCGCTTGCTGCACGCACC
CGCTGGACCTGCTCAAGGTGCATCTACAGACCCAACAGGAGGTGAAGCTGCGCATGACTGGAATGGCACT
GCAGGTGGTGCGAACCGATGGCTTCCTGGCGCTCTACAACGGCCTGAGTGCCTCGCTGTGCAGGCAGATG
ACCTACTCTCTGACTCGGTTCGCAATCTACGAGACCATGCGGGACTACATGACCAAGGACTCCCAGGGGC
CTCTCCCCTTCTACAACAAGGTGTTGCTGGGCGGCATCAGTGGTTTAACTGGAGGCTTCGTGGGGACCCC
AGCAGATTTGGTCAATGTCAGGATGCAGAACGACATGAAGCTGCCCCCGAGCCAACGACGCAACTACTCT
CATGCCCTGGATGGTCTGTACCGTGTAGCCCGTGAAGAAAGCCTGAGGAAGCTCTTCTCTGGAGCAACTA
TGGCGTCCAG
Notes:The probe template was PCR amplified from IMAGE:2192838 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2192838 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16831 same embryo
 EMAGE:16833 same embryo
 EMAGE:16832 same embryo
 EMAGE:16830 same embryo
 EMAGE:16829 same embryo
 EurExpress:euxassay_008673 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS