EMAGE mouse gene expression spatial database

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EMAGE:17007

Suz12 suppressor of zeste 12 homolog (Drosophila) ( MGI:1261758)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011822. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011822_01	euxassay_011822_02	euxassay_011822_03	euxassay_011822_04

euxassay_011822_05	euxassay_011822_06	euxassay_011822_07	euxassay_011822_08	euxassay_011822_09

euxassay_011822_10	euxassay_011822_11	euxassay_011822_12	euxassay_011822_13	euxassay_011822_14

euxassay_011822_15	euxassay_011822_16	euxassay_011822_17	euxassay_011822_18	euxassay_011822_19

euxassay_011822_20	euxassay_011822_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17007_wholemount_strong.wlz

17007_wholemount_moderate.wlz

17007_wholemount_weak.wlz

17007_wholemount_possible.wlz

17007_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17007_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

weak

regional

weak expression: see section 01 02 03 04 18 19 20

hip

weak

regional

weak expression: see section 08 13 14

fibula

weak

regional

weak expression: see section 02

tibia

weak

regional

weak expression: see section 01 02

femur

weak

regional

weak expression: see section 01 02 03 04 05 16 17 18

meckel's cartilage

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

basisphenoid bone

weak

regional

weak expression: see section 13 14

exoccipital bone

moderate

regional

moderate expression: see section 01 02 05 18 19 weak expression: see section 06

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 05 18 19 20 21 weak expression: see section 06

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 05 16 19 20 21 weak expression: see section 06 07 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 03 04 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2951
Entity Detected:	Suz12, suppressor of zeste 12 homolog (Drosophila) ( MGI:1261758)
Sequence:	sense strand is shown >T2951 GGCCTCNAGGCCAGATTCGGCACGAGGATAAGCTTTCTCATGGTGTCTTCAGAGATTTATAAGTGTAAAT ACTGATTTGGCTGGTCTTTATGATGTGTTTAACTGTGAGGCTATTTAAATAGTGTGGATGTGACTTGTCA TCCAGTATTAAGTTCTTAGTTACTGGTTTTTGTGTTAAAAAAAAATAAGAAAGAGGGAAGCTGCAGTTTT CGTTGTAGGCTCCCTAGCTGGTAAACTCTCCTCACGAGTTCACTGAACTCTGATTTTTGCCTCTGGGTAG TGGGTTCTGAGCATTTCTCCTGGGCTTTAATTTGCTAAAGCTGTGCACATATGTAAAAATCAAAAAAATA GATTATTTTAGGGAAGATGTAGGTGTAGAATTATTGCTTATGTCATTTCTTAAAGCAGTTATGCTCTTAA TGCTTAAAAGAAGGCTAGCATTGTTTGCACAAAAAATTGGTGATTTCCCTCCCCTGAACAGTAATGAAAT TACTTCTGTTGAGTAAACTTTTTATGTCATCTTATAATAAAAGCTAAAAATGCCCTTTGGTTCTATTTAT AAAAAAAAAAAAGCTTT
Notes:	The probe template was PCR amplified from IMAGE:1532114 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1532114 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17006 same embryo
	EMAGE:17008 same embryo
	EMAGE:17009 same embryo
	EurExpress:euxassay_011822 same experiment
	MGI:4828545 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source