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EMAGE:17047

Ncstn nicastrin ( MGI:1891700)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047
"Pseudo-wholemount" of euxassay_003289. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003289_01 euxassay_003289_02 euxassay_003289_03 euxassay_003289_04
EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047
euxassay_003289_05 euxassay_003289_06 euxassay_003289_07 euxassay_003289_08 euxassay_003289_09
EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047
euxassay_003289_10 euxassay_003289_11 euxassay_003289_12 euxassay_003289_13 euxassay_003289_14
EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047 EMAGE:17047
euxassay_003289_15 euxassay_003289_16 euxassay_003289_17 euxassay_003289_18 euxassay_003289_19
EMAGE:17047 EMAGE:17047
euxassay_003289_20 euxassay_003289_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17047Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17047_wholemount_strong.wlz
17047_wholemount_moderate.wlz
17047_wholemount_weak.wlz
17047_wholemount_possible.wlz
17047_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17047_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3231
Entity Detected:Ncstn, nicastrin ( MGI:1891700)
Sequence:sense strand is shown

>T3231
TGGCCTCGAGCCAGATTCGGACGAGGCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAATGAGAGCA
AGGATTTATATGAATACTCGTGGGTACAAGGCCCTTGGAATTCCAACAGGACAGAGAGGCTCCCACAGTG
TGTGCGCTCCACAGTGCGACTGGCCAGGGCCTTGTCCCCTGCCTTTGAACTGAGTCAGTGGAGCTCCACA
GAATACTCTACGTGGGCGGAGAGCCGCTGGAAAGACATCCAAGCTCGGATATTCCTAATTGCCAGCAAAG
AGCTTGAGTTCATCACGCTGATCGTGGGCTTCAGCATCCTTATCTTCTCTCTCATCGTCACCTACTGCAT
CAATGCCAAAGCCGACGTCCTTTTCGTTGCTCCCCGAGAGCCAGGAGCTGTGTCTTACTGAAGAGGACTC
TAGCTCTGCCTGCCTGCTCTGAACTTTACTTCCCAGACCAGGTGTCCGGCTGGGAACAAACCACTAATTT
GTCACTGGACTGTCTCTGGGCCTGCTTAGACGGGGATTGACACACAGAATGGAACTGTGAGTGGAGAGAT
GA
Notes:The probe template was PCR amplified from IMAGE:2749183 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2749183 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17048 same embryo
 EMAGE:17049 same embryo
 EMAGE:17051 same embryo
 EMAGE:17050 same embryo
 EMAGE:17046 same embryo
 EurExpress:euxassay_003289 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS