EMAGE mouse gene expression spatial database

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EMAGE:17074

Prkar1a protein kinase, cAMP dependent regulatory, type I, alpha ( MGI:104878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001469_01	euxassay_001469_02	euxassay_001469_03	euxassay_001469_04	euxassay_001469_05

euxassay_001469_06	euxassay_001469_07	euxassay_001469_08	euxassay_001469_09	euxassay_001469_10

euxassay_001469_11	euxassay_001469_12	euxassay_001469_13	euxassay_001469_14	euxassay_001469_15

euxassay_001469_16	euxassay_001469_17	euxassay_001469_18	euxassay_001469_19	euxassay_001469_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17074_wholemount_strong.wlz

17074_wholemount_moderate.wlz

17074_wholemount_weak.wlz

17074_wholemount_possible.wlz

17074_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17074_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 weak expression: see section 04 05 06 07 15 16 17 18

spinal cord

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 15

cervical ganglion

moderate

regional

moderate expression: see section 07 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11

tail dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1317
Entity Detected:	Prkar1a, protein kinase, cAMP dependent regulatory, type I, alpha ( MGI:104878)
Sequence:	sense strand is shown >T1317 CCTCGAGCCTGTTGGCCTACTGGATACTAAAAGTTGCTTTATTGCACCATTTTTCATTGGGAGCGTTATT AAATGCTCATTCACAAATAAATAGAGAGTTGTATGGACTTGGCTGTCACCGCTTCTCTGTGCAGTGCTCG TGTTCAACCTGGGCAGCAAGTGCCGCGCTTTTTGCTGAGGGCGGTCCCTGCACCACTGAATTACCGTAAT GATGTAATAGCTGTGCAGGGCTTTTAAGTGACACAGGTTTCCATTTCCATATGACACAAGTGACATACTT AGACTGTGGCCATGTTTGCTGTATTTCTTTATAGAGTAAATGATTTTAACCTTTAAGGATTAGAAGAATG AATATAGAAAACTCCTAGCACAGTAGAAAGACATCTGCCTGTAATTAAGCTAGTTTAAGTGTGGGAGTGC CCATGTTTTGCTAATCACCAGGCACTGCAGCTAGTTCAGCCCTCTCAGCCTGGTTTGGTGTGTCTGTTGC TAGTGTTTCTTCTCCAACCCTGAAATGTTTTGGTGTGGCTGGCCGTCTATCCCTGCCTTTAAGTTTGAAA TCATTTGTAGATGGCAAGTATTGGTT
Notes:	The probe template was PCR amplified from IMAGE:2270110 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2270110 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17073 same embryo
	EMAGE:17075 same embryo
	EMAGE:17070 same embryo
	EMAGE:17072 same embryo
	EMAGE:17071 same embryo
	EurExpress:euxassay_001469 same experiment
	MGI:4827418 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source