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EMAGE:17075

Fn1 fibronectin 1 ( MGI:95566)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17075 EMAGE:17075 EMAGE:17075 EMAGE:17075 EMAGE:17075
euxassay_001464_01 euxassay_001464_02 euxassay_001464_03 euxassay_001464_04 euxassay_001464_05
EMAGE:17075 EMAGE:17075 EMAGE:17075 EMAGE:17075 EMAGE:17075
euxassay_001464_06 euxassay_001464_07 euxassay_001464_08 euxassay_001464_09 euxassay_001464_10
EMAGE:17075 EMAGE:17075 EMAGE:17075 EMAGE:17075 EMAGE:17075
euxassay_001464_11 euxassay_001464_12 euxassay_001464_13 euxassay_001464_14 euxassay_001464_15
EMAGE:17075 EMAGE:17075
euxassay_001464_16 euxassay_001464_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17075Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17075_wholemount_strong.wlz
17075_wholemount_moderate.wlz
17075_wholemount_weak.wlz
17075_wholemount_possible.wlz
17075_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17075_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2923
Entity Detected:Fn1, fibronectin 1 ( MGI:95566)
Sequence:sense strand is shown

>T2923
TCAGCTTCAGCACAATTTACAGCTTCTCCAAGCATCGCCCCGTGGGATGTTTTGAGACTTCTCTCCTCAA
TGGTGACAGTTGGTCACCCTGTTCTGCTTCAGGGTTTCAGTACTGCTCAGTGTTGTTTAAGAGAATCAAA
AGTTCTTATGGTTTGGTCTGGGATCAATAGGGAAACACAGGTAGCCAACTAGGAGGAAATGTACTGAATG
CTAGTACCCAAGACCTTGAGCAGGAAAGTCACCCAGACACCTCTGCTTTCTTTTGCCATCTGACCTGCAG
CACTGTCAGGACATGGCCTGTGGCTGTGTGTTCAAACACCCCTCCCACAGGACTCACTTTGTCCCAACAA
TTCAGATTGCCTAGAAATACCTTTCTCTTACCTGTTTGTTATTTATCAATTTTTCCCAGTATTTTTATAC
GGAAAAAATTGTATTGAAGACACTTTGTATGCAGTTGATAAGAGGAATTCAGTATAATTATGGTTGGTGA
TTATTTTTATAAGCACATGCCAACGCTTTACTACTGTGGA
Notes:The probe template was PCR amplified from IMAGE:1531533 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1531533 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17073 same embryo
 EMAGE:17070 same embryo
 EMAGE:17072 same embryo
 EMAGE:17074 same embryo
 EMAGE:17071 same embryo
 EurExpress:euxassay_001464 same experiment
 MGI:4824887 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS