EMAGE mouse gene expression spatial database

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EMAGE:17087

Plekha4 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 ( MGI:1916467)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008858_01	euxassay_008858_02	euxassay_008858_03	euxassay_008858_04

euxassay_008858_05	euxassay_008858_06	euxassay_008858_07	euxassay_008858_08	euxassay_008858_09

euxassay_008858_10	euxassay_008858_11	euxassay_008858_12	euxassay_008858_13	euxassay_008858_14

euxassay_008858_15	euxassay_008858_16	euxassay_008858_17	euxassay_008858_18	euxassay_008858_19

euxassay_008858_20	euxassay_008858_21	euxassay_008858_22	euxassay_008858_23	euxassay_008858_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17087_wholemount_strong.wlz

17087_wholemount_moderate.wlz

17087_wholemount_weak.wlz

17087_wholemount_possible.wlz

17087_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17087_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

diencephalon roof plate

strong

regional

strong expression: see section 15 16 17

choroid invagination

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 18 19 20

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

strong

regional

strong expression: see section 04 05 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 17 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 09 19 20

peripheral nervous system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 14 15 16

brachial plexus

strong

regional

strong expression: see section 07 15

lumbosacral plexus

strong

regional

strong expression: see section 11 14

naris

strong

regional

strong expression: see section 14 15 16 17 18

viscerocranium

strong

regional

Expression in the turbinate bone.

esophagus

strong

regional

strong expression: see section 11 12

tongue

strong

regional

strong expression: see section 11 12 13 14 15 16 17

laryngeal cartilage

strong

regional

strong expression: see section 12 13

thyroid cartilage

strong

regional

strong expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4456
Entity Detected:	Plekha4, pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4 ( MGI:1916467)
Sequence:	sense strand is shown >T4456 CAGGGGAGAGACAGAGAGAGGGAGAGAAAGAACTCACAAGTGAGACAGAGAAGAAAGCAGATATCAGGTG AAGAGGGAGACCATTCTGAGAGGCACAAAAAGAGGTGGTGACAGATCCTGTCACCAGGACACCAGAACTG GCGCTGGTTTTACAGGCGTGTGCTGCCCTCCTCAGCCTCCTTGCCTGCCTTCTGAGAGAAATCCCTTTGG GAGCCAGTCCCAGGAAAAGCCCTCATTCATTTCTGTTCCTACTGCACCCCAGAAGCCTACCCGTGCAGTA CACAAGGTCCATGCCTTTGGAAAAAGGAACAATGCACTTCGACGAGACCCCAACTTGCCGGTGCACATCC GTGGCTGGCTGCACAAACAGGACAGCTCAGGCCTCCGGCTGTGGAAGCGCCGTTGGTTCGTCCTTTCAGG CCATTGCCTCTTCTACTATAAGGGCAGAGCACCCGGGCATGAGGACCTATGTCCTTGCAGCTGACACATT AGAGGACCTGAGAGGTTGGCTTCGAGCACTGGGGAGGGCCTCTCGTGCGGAGGGGGAAGATTGCGGACTC CCGAGGTCACCTGCACGTCCCAGGCCTGGGGAAGGTCCTGGGGGACCCGGAGGACCCCCAGAGGTGAACA GAAGGGAAGAGGGGCGCATCTCAGAATCACCAGAGGTAGCTCGGCTCTCCAGAGGTCTTGGCAGACATGA AACGCACACTCCCAACTCTACAGTGGATCTGCAGACTGACACCTGGAGTCGTAGAACCCGGAGCCCAGAG TTGTTCTCACCCCTTTCCCGGCCTCCCTCCCCTCTGAGCCTTCCCCGTCCTCGCTCTGCCCCGGCCCGGC GACCCCCTCTCTCAGCAGGAGACATCTCATTTCCTGCCCGACCGCATACTCCTTTGAGTCGCATCGATGT CCGACCTCCTCTGGATTGGGGACCCCAAAGACAGACCCTCTCCCGACCTCCCATTCCACGCCGAGGACCT TCCTCTGAAGCTGGCGGAGAACGGCCACCCAGGAGTCCCCAGCCCCGGACACCAGAGCAGAGAACGCAGT CTACCCAGGCCACCTCTGGTTCGTCCACCTATCTCCAGCTGCCCCCAAGACCTCCTGGGACCCAGGCCTC CATGATTTTGCTGCCAGGACCCCCTGTGGACTCAGCTTTGCATCAGAGCTTGGAGACAGATACGTTGTTG ACTAAGCTGTGTGGGCAGGACCGGCTCCTGAGGCAGCTACAGGAGGATCTGGACAAGAGGCAGGAGGAGA AGGAGCAGCTGGAGGCAGCTTTGGAGCTGACCCGGCAGCAGCTGGGCCAAGCTACCAGGGAGGCTGCAGC TTCCGGGAAGGCCTGGGGTCGCCAGCGCCTTCTGCAGGACCGGCTGGTGAACGTGAGGGCTGCTCTGTGT CACCTGGCTCAGGAGCGAGAGCGAGTTTGGGACACATACAGCGGCCTGGAGCAGGACCTCGGCACCCTAA GAGAGACGCTCGAGTACCTGCTGCACCTCGGGTCTCCCCAGGACCGTGCGTGTGCTCAACAGCAGCTGTG GATGGTGGAGGACACGCTGGCAGGTCTGGGTGGCCCCCAGAAACAGCCCCCACATACTGATCCCAAGTCT CCATCCCCTGCACCCCAAGGAGAAGAGTCCTCAGAGAGGGAGACCCTGGAATGGAACTTAAATCGTCAGA CTTGGTGGCAAGTACCCTTATCTGCGGAGATATCTCACCAGCCCCTTATTTAGTAAATAGCCTTTCAGAG TCACTGGAGCTCAGCTCCCCTCAGTCCCCTGAGGTGGACTGGAGTCGTCCCTCAGGCGGTGACAGAGCCC TCAGCAGCCCTCAGTCAGGTGTTGGGTCTCCAAGGGTCTCCCGGGCATCCAGCCCTGAATGTCGTCAACC CTCATCTCCACTGCTGAGAACTAAGGCCCCGCTGGCGCGGCCTCGTATGAGCGCGCAGGAGCAGCTGGAG CGCATGCGTAGAAACCAAGCATGCGGGCTCCCTCTTCCTCGCCCTACCTCCCCGAGGCTCCTGACCCTGG GGAGGACACTGTCCCCAGCTCCACGCCAGCCTGACATGGAACAGAGGTCCCAGGCACTCTGCAACCTGCT CACCAGTACCTGGAGGCCACCGGGAGCGAGTGCTCAGCCTCTCCCAGGCTCTAGCTACAGAGGCTTCCCA GTGGCACAGGCTGATGGCAGCCTCTCCAGGCAGAAATTTGGATACCAGGGGAGACTGTCTCCAGCCAAGC CCACAGCCTCCCAGTGAAGAACTGCCCCAGGTCACCTCGTCCCCAGCTCCTCACAAGGCTACTTCTGCTA CCACAGGGTTCTCATGCCAAGGCAGCCGGAGAGGCCTGGCCCCATGGGAGGCCAGGTGGGACCCTGGGAA GGCCCCTCCAGCTCTGACCCAGGAAGAGGGGGCATGGCCCTTCAGAGTCACTCTGCTGCAGTCCAGCTTT TGACTCCCATCTTTATACTGAGCCCTACAAGCTGGAAGCACAACTTTGAGGTGTGGGACCATTCAGGACC ACAGGGGAGAGGTTCATTCCAGCATGTATTCTAGGAGGGCCAGGGCCGCTATGGTCAAAGATTGATTTTC CCAACACTGTCCAAAATTGGATGAAAACTTTGAACTATTAGTCAATAAATAGCTTTTATTCTCTCAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4945500 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4945500 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17086 same embryo
	EMAGE:17088 same embryo
	EurExpress:euxassay_008858 same experiment
	MGI:4827265 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source