EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17115

Dis3 DIS3 mitotic control homolog (S. cerevisiae) ( MGI:1919912)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001502. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001502_01	euxassay_001502_02	euxassay_001502_03	euxassay_001502_04

euxassay_001502_05	euxassay_001502_06	euxassay_001502_07	euxassay_001502_08	euxassay_001502_09

euxassay_001502_10	euxassay_001502_11	euxassay_001502_12	euxassay_001502_13	euxassay_001502_14

euxassay_001502_15	euxassay_001502_16	euxassay_001502_17	euxassay_001502_18	euxassay_001502_19

euxassay_001502_20	euxassay_001502_21	euxassay_001502_22	euxassay_001502_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17115_wholemount_strong.wlz

17115_wholemount_moderate.wlz

17115_wholemount_weak.wlz

17115_wholemount_possible.wlz

17115_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17115_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 12 weak expression: see section 11 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 07 08 09 10 11 12 14 15 16 17 18 20 21 weak expression: see section 05 06

renal cortex

moderate

regional

moderate expression: see section 05 06 07 08 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4498
Entity Detected:	Dis3, DIS3 mitotic control homolog (S. cerevisiae) ( MGI:1919912)
Sequence:	sense strand is shown >T4498 GCGGGAATGCGCGTGGCCGCGCCTCGGTTTCTTAGCTGACGGCCGGGATCCCACGCCGCGAGGCGAGATG CTCAGGTCCAAGACGTTCTTGAAGAAGACCCGCGCGGGCGGCGTGGTGAAGATCGTGCGCGAGCACTACC TGCGGGATGACATCGGCTGCGGCGCGCCGGCTTGCTCGGCCTGCGGCGGGGCGCACGCGGGCCCGGCCCT GGAGCTGCAGCCCCGGGACCAGGCGAGCAGCCTCTGCCCGTGGCCGCACTACCTTCTGCCGGACACCAAT GTGCTGCTGCACCAGATTGATGTCCTCGAACACCCGGCCATCAAAAATGTCATTGTGCTACAAACAGTGA TGCAAGAAGTGAGAAACCGGAGCGCCCCCATCTACAAGCGAATCAGGGATGTGACCAATAACCAGGAAAA GCATTTCTATACCTTCACTAATGAGCACCATAAAGAAACCTACATCGAGCAAGAGCAGGGAGAGAATGCC AATGACAGGAATGACAGAGCCATCCGAGTCGCAGCGAAGTGGTACAACGAGCACCTGAAGAGGGTGGCAG CAGACAGTCAGCTGCAAGTTATCCTGATAACCAATGACAGGAAGAACAAAGAGAAAGCTGTGCAAGAGGG GATACCAGCCTTCACGTGTGAAGAATACGTAAAGAGCCTGACTGCTAACCCTGAACTTATAGACCGTCTT GCTTACTTGTCCGATGAAATGAATGAAATAGAAAGTGGGAAAATAATATTTTCAGAGCATCTTCCCTTAA GCAAGCTCCAACAAGGCATAAAATCTGGTTCCTATCTTCAAGGAACATTCAGAGCTAGCAGGGAAAATTA TTTGGAGGCTACAGTATGGATTCATGGAGACAAAGAAGAGGAAAAAGAGATACTTATACAGGGAATTAAG CATCTAAACAGAGCTGTGCATGAAGACATTGTGGCCGTGGAGCTACTGCCCAGGAGCCAGTGGGTGGCAC CGTCTTCCGTGGTTTTAGACGATGAAGGTCAAAATGAAGACGATGTGGAGAAAGATGAGGAGAGAGAACT CCTGCTTAAGACTGCTGTAAGTGAAAAAATGTTACGGCCTACAGGTCGAGTTGTGGGGATAATAAAAAGG AACTGGAGACCGTATTGTGGCATGCTTTCCAAGTCTGATATTAAGGAGTCAAGAAGACATCTCTTTACAC CCGCTGATAAGAGAATTCCACGAATTCGGATAGAAACCAGACAGGCTTCTGCGTTAGAAGGACGGAGAAT TATTGTCGCTATTGATGGTTGGCCTAGAAATTCTAGATATCCAAATGGACACTTTGTAAAGAATTTAGGC GATGTTGGAGAGAAGGAGACAGAAACGGAAGTGTTGCTGCTCGAGCACGATGTTCCTCATCAGCCCTTTT CCCAGGCTGTGCTTAGCTTCCTGCCCAGGATGCCCTGGAGCATTACTGAGGAGGACATGAAAAACCGAGA AGACCTGAGACATCTGTGTGTTTGCAGTGTGGACCCTCCAGGGTGCACTGACATAGATGACGCTCTGCAT TGTAGAGAGCTCAGCAATGGAAACTTGGAGGTTGGTGTTCATATTGCGGATGTTAGCCATTTTATCAGGC CAGGAAATGCGTTGGATCAAGAATCTGCAAGAAGAGGAACAACTGTTTATCTTTGTGAAAAGAGGATTGA CATGGTTCCAGAGTTGCTCAGCTCCAACCTCTGTTCCTTAAGATCCAACGTTGACAGGCTTCTCTTACGT ACGCGGAAGCACAGATGAGAATTGATTCGGCGGCTATGAATGATGATATTACCACTAGTCTCCGTGGACT CAATCAGCTGGCTAAAATTCTAAAAAAGGGAAGGATTGAAAAGGGGGCTTTGACTCTGTCTTCTCCAGAG ATCCGATTCCACATGGACAGTGAAACCCACGACCCAATCGACCTGCAGACGAAAGAGCTGAGAGAAACAA ATTCCATGGTGGAAGAATTTATGTTACTTGCTAATATTTCTGTCGCAAAAAAAATTCATGAAGAGTTTTC TGAACATGCTCTGCTTCGGAAACACCCAGCTCCGCCTCCCTCGAATTATGACATTCTTGTTAAGGCTGCA AAGTCCAAGAATTTGCAAATTAAGACTGATACAGCCAAATCTTTGGCCGACTCTTTGGACCGGGCTGAAT CTCCGGATTTCCCATACCTGAATACTCTATTAAGGATACTGGCCACTCGCTGTATGATGCAAGCTGTGTA CTTCTGCTCTGGGATGGATAATGATTTTCATCACTATGGCTTAGCCTCCCCCATATACACACATTTCACC TCTCCTATCAGAAGATATGCAGACATAATTGTCCATCGGCTATTAGCTGTGGCGATTGGGGCTGACTGTA CTTACCCTGAGTTGACAGACAAACACAAGCTTTCAGATATATGTAAAAACCTCAATTTCCGGCATAAAAT GGCTCAGTATGCCCAGCGTGCTTCGGTGGCTTTTCATACACAGTTGTTTTTCAAAAGCAAAGGAATAGTA AGTGAAGAAGCCTATATTCTCTTTGTAAGGAAAAATGCAATTGTGGTGTTAATTCCAAAGTATGGCTTAG AAGGTACAGTTTTCTTTGAAGAAAAAGATAAACCAAAGCCACGCCTTGCTTACGATGATGAGATCCCTTC CCTGAGAATAGAAGGTACAGTGTTCCATGTGTTTGATAAGGTTAAAGTGAAGATCACATTAGATTCATCA AATCTTCAACATCAGAAAATCCGCATGGCCCTTGTAGAACCACAGATCCCAGGAATAAATATTCCTCCTA ATGTTGCAGACAAGGCTCTTACTGCACCAGGGGGAAAGAAGAGGAAGCTTGAGAAGTAGCTACGTTCAAC AGAACATCCAAAGACTCAACAGCAACAAAATGGAGAGCATACTTACAGACTCCTGTGCGATAGCTACTTT TAAATACATTTCAATAATTTTAACAAATTTGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4952415 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4952415 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17114 same embryo
	EMAGE:17112 same embryo
	EMAGE:17113 same embryo
	EMAGE:17116 same embryo
	EurExpress:euxassay_001502 same experiment
	MGI:4824311 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source