EMAGE mouse gene expression spatial database

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EMAGE:17116

Ccm2 cerebral cavernous malformation 2 homolog (human) ( MGI:2384924)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001506. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001506_01	euxassay_001506_02	euxassay_001506_03	euxassay_001506_04

euxassay_001506_05	euxassay_001506_06	euxassay_001506_07	euxassay_001506_08	euxassay_001506_09

euxassay_001506_10	euxassay_001506_11	euxassay_001506_12	euxassay_001506_13	euxassay_001506_14

euxassay_001506_15	euxassay_001506_16	euxassay_001506_17	euxassay_001506_18	euxassay_001506_19

euxassay_001506_20	euxassay_001506_21	euxassay_001506_22	euxassay_001506_23	euxassay_001506_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17116_wholemount_strong.wlz

17116_wholemount_moderate.wlz

17116_wholemount_weak.wlz

17116_wholemount_possible.wlz

17116_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17116_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

tail dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4496
Entity Detected:	Ccm2, cerebral cavernous malformation 2 homolog (human) ( MGI:2384924)
Sequence:	sense strand is shown >T4496 CAGTGGGGCTCCCGGGGCGGGCCGCGGGGGCCGCACGCGGCGACATGGAGGAGGAGGGCAAGAAGGGCAA GAAGCCTGGAATTGTGTCGCCATTTAAACGAGTATTCCTAAAAGGTGAAAAGAGTAGAGATAAGAAAGCC CATGAGAAGGTGACAGAGAGGCGCCCTCTGCACACTGTGGTGCTGGCGTTACCTGAGCGCGTCGAGCCAG ACAGACTGCTGAGCGACTATATTGAGAAGGAGGTGAAGTATTTAGGTCAGTTAACATCCATTCCCGGCTA CCTGAACCCTTCCAGTAGGACGGAAATCCTGCATTTCATAGACAAGGCAAAGCGGTCCCACCAGCTTCCT GGGCACCTGACTCAGGAGCACGATGCTGTGCTCAGTCTGTCTGCCTACAATGTCAAGTTGGCCTGGAGGG ACGGGGAGGACATTATCCTCAGGGTGCCCATCCATGATATCGCTGCTGTCTCCTATGTCCGAGATGATGC TGCACACCTGGTGGTCCTGAAGACAGCCCAGGACCCAGGCATCTCTCCCAGCCAGAGTCTGTGTGCAGAA AGTTCTAGAGGCCTCAGCGCAGGTTCCTTGTCAGAAAGTGCAGTGGGGCCAGTAGAGGCATGTTGCCTGG TCATCATGGCCACAGAGAGCAAGGTCGCCGCTGAAGAGTTGTGCTCCCTGCTCAGCCAGGTCTTCCAGAT TGTTTACACGGAGTCCACCATCGACTTTCTGGACCGAGCAATATTTGATGGGGCTTCCACACCTACCCAC CACCTGTCGCTGCACAGTGATGACTCTTCCACGAAAGTGGACATGAAGGACAGTTACGATGCTGACGCCA GCACCTTCTGCTTCCCGGACTCTGGGGATGTGGGAGGCCTGCCGCCCTTACCCTTCTGCATGCAGACATC ACCCCATAGCAAGACTGTCAGTGAGAGCGAGCTGAGCACCAGCGCCACGGAACTGCTGCAGGACTACATG CTCACGTTACGTACGAAGCTGTCATCACAGGAGATCCAGCAGTTCGCAGCTCTGCTACATGAGTACCGCA ATGGGGCCTCTATCCATGAGTTTTGCATCAGCCTGCGGCAGCTCTATGGGGACAGCCGCAAGTTCCTGCT ACTTGGTCTCAGACCCTTCATACCTGAGAAGGACAGTCAGCACTTTGAAAACTTCCTGGAGACCATTGGC GTGAAAGACGGCCGTGGCATCATCACTGACAGCTTTGGTAGGCATCGTCGTGCCCTGAGTACCACCTCCA CATCCACCATCAATGGGAACAGGACCACAGGCAGCCCTGATGACCGCTCTGCGCCCTCAGAGGGGGATGA GTGGGACCGCATGATTTCAGACATCAGTAGTGATATTGAAGCGCTAGGCTGCAGCATGGACCAGGACTCA GCGTGAAGGAGCGGGACAGCACACACTTGTCTCACACAGCCATCCCAGGCCTTCCTGGGAGGAGTGCCCA GACCTGAGTGCCAGCCTTTCCCAGCTTGGAGCTAGGGAGGGGCTGAAGAGAAAGGTAGCTCTGGTGGCCA GGTCCTCTACTGTGAAGGAGTGGAGAGCTGCCACAGGACATGTGCCCCACAGGGCAGAAACCTCTGTGCC TATTCCTCAGGGGCTCAGCAATGCCTGTAGCATATCCCCACTCGGGGAGGGCCAGGGGCAGCTGGCAGGG GAGCCAGTCCTGCCGGCCTGGCCCTGGACAGCTGATATTTGCACATGACTTTCCTCTTCTACTTCTCAGA GACCTTAAAAACAAGTTTACTACAATGTGAATAATTTAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4952288 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4952288 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17114 same embryo
	EMAGE:17112 same embryo
	EMAGE:17113 same embryo
	EMAGE:17115 same embryo
	EurExpress:euxassay_001506 same experiment
	MGI:4823705 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source