EMAGE mouse gene expression spatial database

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EMAGE:17136

Acad9 acyl-Coenzyme A dehydrogenase family, member 9 ( MGI:1914272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001518_01	euxassay_001518_02	euxassay_001518_03	euxassay_001518_04	euxassay_001518_05

euxassay_001518_06	euxassay_001518_07	euxassay_001518_08	euxassay_001518_09	euxassay_001518_10

euxassay_001518_11	euxassay_001518_12	euxassay_001518_13	euxassay_001518_14	euxassay_001518_15

euxassay_001518_16	euxassay_001518_17	euxassay_001518_18	euxassay_001518_19	euxassay_001518_20

euxassay_001518_21	euxassay_001518_22	euxassay_001518_23	euxassay_001518_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17136_wholemount_strong.wlz

17136_wholemount_moderate.wlz

17136_wholemount_weak.wlz

17136_wholemount_possible.wlz

17136_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17136_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4523
Entity Detected:	Acad9, acyl-Coenzyme A dehydrogenase family, member 9 ( MGI:1914272)
Sequence:	sense strand is shown >T4523 AGCGGCGGGGCTTCCAGCGACTTGAAGTCTGAAGTCAGGAGCAGCATGTCTGGCTGTGTGCTGCTCTCGC GTGGGGCGACTGCGGCGGCGGCTGCGGCGCGAGCTTCCCGGGTGCTGAGGGAATTCACGGCGAGGCGCCG CCCGCTGCACACCAGCCTGCAGAGCTGCTCGTTCGCCAAGGAGCTCTTCCTGGGCAACATCAAGCAGAAA GGAGTTTTCCCATTTCCAGAGGTCAGTCAACATGAACTTAGTGAAATCAATCAGTTCGTGGGACCGCTGG AAAAATTCTTCACTGAAGAAGTGGACTCTCGAAAAATTGACCAGGAGGGAAAAATCCCAGTTGACACCTT AGAGAAGTTGAAGAGCCTGGGACTTTTTGGCATACAGGTCCCAGAAGAATATGGTGGCCTGGGCCTCTCC AACACCATGTATGCTCGGCTCGGGGAGATCATCAGCCTGGATGCCTCTATCACGGTGACCCTGGCAGCAC ACCAGGCTATCGGCCTCAAGGGGATTATCTTGGTCGGCAATGACGAACAGAAGGCCAAGTATCTGCCCAA ACTGTCGTCTGGGGAACACATCGCAGCCTTCTGCCTAACAGAGCCAGCCAGTGGGAGTGATGCTGCATCC ATCCAGACCAGAGCTACGTTAAGTGAAGATAAGAAGTACTTCATCCTCAATGGCTCCAAGGTTTGGATCA CCAATGGAGGACTGGCCAATATTTTTACTGTGTTTGCAAAAACCGAGGTGGTTGATTCTGATGGTTCGAA AACAGACAAAATGACCGCATTCATAGTAGAAAGAGACTTCGGCGGAATCACTAATGGGAAACCGGAAGAT AAATTAGGCATTCGAGGCTCCAACACATGTGAGGTCCATTTTGAAAATACCAGAGTGCCTGTGGAAAATG TCCTTGGAGAAGTTGGAGGGGGCTTTAAGGTAGCCATGAACATCCTGAACAGTGGGCGATTCAGCATGGG CAGTGCTGTGGCCGGGATGCTGAAGAAGCTGATCGAACTGACTGCTGAGTATGCCTGTACGAGGAAACAG TTCAACAGGAATCTCAGTGAATTTGGGTTGATTCAGGAAAAGTTTGCGCTCATGGCTCAGAAGGCATACG TAATGGAGAGTATGGCTTACCTCACTTCAGGGATGCTAGACCAACCAGGATTTCCCGACTGCTCTATCGA GGCAGCCATGGTGAAGGTGTTTAGCTCTGAGGCTGCCTGGCAGTGTGTGAGCGAGGCTCTGCAGATCCTT GGGGGCTCAGGCTACATGAAGGACTACCCCTATGAGCGCATGCTGCGTGATGCCCGCATTCTCCTAATCT TTGAGGGAACCAATGAGATTCTTCGGTTGTTCATTGCCCTGACAGGCCTGCAGCATGCTGGACGCATCCT GACCTCGAGGATCAAAGAGCTTAAAAGTGGCAATGTGACCACAGTCATGGAGACGATTGGTCGGAAACTT CGGGATTCTCTGGGCCGAACTGTGGACCTGGGGCTAACAGGCGATCTTGGTGTTGTCCACCCCAGCCTTG GCGACAGTGCCAACAAGCTTGAGGAAAATGTCCATTACTTTGGCCGGACTGTTGAGACCCTGTTACTCCG TTTTGGAAAGAACATTGTAGAGGAACAGCTGGTGTTAAAGCGGGTAGCCAACATCCTCATCAACTTGTAT GGCATGACTGCTGTGCTGTCGAGAGCCAGCCGCTCCATCAGAATTGGGCTCAGGAATCATGATCACGAGG TTCTGTTGGCCAACATGTTCTGTGTGGAAGCTTATTTCCAGAATCTCTTCAGCCTGTCTCAGCTGGACAA GAACGCTCCAGAAAACCTGGATGAGCAGATTAAGAAAGTGTCTCGGCAGATCCTTGAGAAGCGAGCCTAT ATCTGTGCCCACCCGCTGGACAGAGCATCCTGAGGCTGGAACCATGTACCTGCCTAGCCCGGGTTAACAT CATTCACAAGACAGACCTTGGGGTCATCTGTGTTGACTGTATGTTATCTGCATACACTAAAAACACTTTC TTCTCTGAGCAAAAGCAATAGTTAGGTTTTGGTCTATAGGCCCTGTATCTAGAGGGATCTCATCTCCTGG AAGGAAGGAAGTACCAGCCAAATTGTTGAAGAAAGAAAGAAAAAGGAAGAACCAAATTGTGAATGGCTCC TGTGTGTGACTGCAATGATACATCTCAGTGGACATGGGCCCACCTTTAAGACACAAGGCTCACAGTAAAC TAAGATTTGGGCAGCTATTCCTTCTAAACCATGTATAAAGAAACTCCTGAGAAGTTGAGTCTATACCACT CACCGAGACTGTTTCCAATCTACGTACGGAATCATTTAACAAAGTATATAAAGTTTATGTCCACCTAGAC TTTATGTTCTTCAGTCCATGACCGTGAGGGGCCATAACAATAAAATCCCTTCTTGAGTGTTTTAAACTTC AAATTGTAGTGCTCCTCCACTTCCTTTGTTAGGCTTATCCCAAGGGGCTGGTTAGGTGCTTTACTTTACT AGAATGTTGTTTGATTCTTTGATAGTTTTATACATTTAATGATGTGTCTTGGTCATATCTATCCTAACTC CTCCGTCCTGTTTTATGGCATCCTAACATGCCCAGCTCCCTACTTCTTCTTTTCAACTCACTGTGTCCCC TTAGTGCTGAGTGGGAATATTAACTTTCTGACTTGCTCTCCTGCATCGATGATTTTTACCCTCTCTTAAA AAGTGTTCTCTTAAAGTAGCCAAGGAGCTAAAGGGATCTGCAACCCTATAGGTGGAACAACATTATGAGC TAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATATATCAAAAGATGGCCTAGTCGGCCATCACTG GAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAGTACAGGGGAACACCAGGGCCAAAAAGGG GGAGTGGGTGGGCAGGGGAGTGGGGGTGGGTGGATATGGGGGACCTTTGGTATAGCATTGGAAATGTAAA TGAGTTAAATACCTAATAAAAAATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4971848 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4971848 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17132 same embryo
	EMAGE:17133 same embryo
	EMAGE:17135 same embryo
	EMAGE:17134 same embryo
	EurExpress:euxassay_001518 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source