EMAGE mouse gene expression spatial database

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EMAGE:17145

Eif3l eukaryotic translation initiation factor 3, subunit L ( MGI:2386251)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001532. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001532_01	euxassay_001532_02	euxassay_001532_03	euxassay_001532_04

euxassay_001532_05	euxassay_001532_06	euxassay_001532_07	euxassay_001532_08	euxassay_001532_09

euxassay_001532_10	euxassay_001532_11	euxassay_001532_12	euxassay_001532_13	euxassay_001532_14

euxassay_001532_15	euxassay_001532_16	euxassay_001532_17	euxassay_001532_18	euxassay_001532_19

euxassay_001532_20	euxassay_001532_21	euxassay_001532_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17145_wholemount_strong.wlz

17145_wholemount_moderate.wlz

17145_wholemount_weak.wlz

17145_wholemount_possible.wlz

17145_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17145_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4542
Entity Detected:	Eif3l, eukaryotic translation initiation factor 3, subunit L ( MGI:2386251)
Sequence:	sense strand is shown >T4542 CGGACGCGTGGGTCGACCCACGCGTCCGGCCGTAGCCATGTCTTACCCCGCTGATGACTACGAGTCTGAG GCTGCTTATGATCCCTACGCCTACCCGGGCGACTATGATATGCACACAGGAGATCCGAAGCAGGACCTTG CCTATGAGCGGCAGTATGAGCAGCAGACATATCAGGTGATACCGGAAGTGATCAAAAACTTCATCCAGTA CTTCCACAAGACCGTCTCCGATTTGATTGACCAGAAGGTGTACGAGCTTCAGGCCAGCCGCGTCTCGAGT GATGTCATTGACCAGAAGGTGTACGAGATCCAGGACATTTACGAGAACAGCTGGACCAAGCTAACTGAAA GGTTCTTCAAGAATACACCGTGGCCTGAGGCAGAAGCAATTGCACCCCAAGTTGGCAATGATGCAGTATT CCTAATTTTGTACAAAGAATTGTACTACAGGCATATTTACGCCAAAGTCAGTGGGGGTCCCTCCTTAGAA CAGAGGTTCGAATCTTATTACAACTACTGCAATCTCTTCAACTACATCCTCAATGCGGATGGACCTGCGC CCCTTGAACTCCCCAACCAGTGGCTCTGGGACATCATCGATGAGTTCATCTACCAGTTCCAGTCATTCAG TCAGTACCGCTGCAAGACGGCCAAGAAGTCAGAGGGAGAGATGGACTTCCTTAGGTCCAACCCTAAAGTC TGGAACGTGCACAGCGTCCTCAACGTGCTGCACTCCCTGGTGGACAAGTCCAACATCAACCGGCAGCTGG AGGTCTACACCAGCGGAGGTGACCCGGAAAGTGTAGCTGGGGAGTATGGACGACACTCCCTCTACAAGAT GCTCGGCTACTTCAGCCTGGTGGGGCTTCTGCGCCTGCATTCTCTACTGGGGGATTACTACCAGGCTATC AAGGTGCTGGAGAATATCGAGCTGAACAAGAAAAGCATGTATTCCCGTGTGCCTGAGTGCCAGGTCACCA CCTACTATTATGTTGGCTTTGCGTATTTAATGATGCGTCGATATCAGGATGCTATCCGAGTCTTTGCCAA CATCCTCCTGTACATCCAGAGGACCAAGAGTATGTTCCAGAGGACCACATACAAATATGAAATGATTAAC AAGCAGAACGAGCAGATGCATGCTCTGCTGGCCATTGCTCTCACCATGTACCCGATGCGGATCGACGAGA GCATACACCTCCAGCTGCGGGAGAAATACGGGGACAAGATGCTGCGCATGCAGAAGGGGGACCCCCAGGT CTATGAGGAACTTTTCAGCTATGCCTGCCCCAAGTTCCTGTCGCCTGTGGTGCCAAACTACGACAATGTC CATCCTAACTACCACAAAGAGCCCTTCCTGCAGCAGCTGAAGGTGTTTTCCGATGAGGTGCAGCAGCAGG CCCAGCTCTCCACCATCCGCAGCTTCCTCAAGCTCTACACCACCATGCCTGTGGCCAAGCTGGCCGGCTT CCTGGACCTCACCGAGCAGGAGTTCCGCATCCAGCTGCTCGTCTTCAAGCACAAGATGAAGAATCTGGTG TGGACCAGTGGCATTTCTGCCCTAGATGGCGAATTCCAGTCGGCCTCGGAGGTGGACTTCTATATTGATA AGGACATGATCCATATTGCAGACACCAAAGTTGCCAGACGCTATGGGGATTTCTTTATCCGACAGATCCA CAAGTTTGAAGAGCTTAATCGAACCCTGAAGAAGATGGGACAGAGGCCCTGAAGATACTCATCCACGAGT CAAGAACCTGTTTTGATATTATAGATGGGAAGCATTCTTGTCACCAAGAAGCCTTACCTAAGTCAGCCGT CAGCTGAATTGAAACTTGTTCAAATCAAGGACTGGATGTTTTAAGGCGCTCTCAGTAAAGGGTCTTTGTT GGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4975621 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4975621 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17146 same embryo
	EMAGE:17148 same embryo
	EMAGE:17147 same embryo
	EurExpress:euxassay_001532 same experiment
	MGI:4824510 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source