EMAGE mouse gene expression spatial database

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EMAGE:17148

Aldob aldolase B, fructose-bisphosphate ( MGI:87995)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001529_01	euxassay_001529_02	euxassay_001529_03	euxassay_001529_04

euxassay_001529_05	euxassay_001529_06	euxassay_001529_07	euxassay_001529_08	euxassay_001529_09

euxassay_001529_10	euxassay_001529_11	euxassay_001529_12	euxassay_001529_13	euxassay_001529_14

euxassay_001529_15	euxassay_001529_16	euxassay_001529_17	euxassay_001529_18	euxassay_001529_19

euxassay_001529_20	euxassay_001529_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17148_wholemount_strong.wlz

17148_wholemount_moderate.wlz

17148_wholemount_weak.wlz

17148_wholemount_possible.wlz

17148_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17148_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

kidney calyx

strong

regional

strong expression: see section 05 06 07 08 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4539
Entity Detected:	Aldob, aldolase B, fructose-bisphosphate ( MGI:87995)
Sequence:	sense strand is shown >T4539 GCCACCTCACACAGCTTCTGATACCTTGGAGGACTCTTCCCCTTTGCTGTACCTGTCATCATGGCTCACC GATTTCCAGCCCTCACCCCAGAGCAGAAGAAGGAGCTCTCTGAAATTGCCCAGCGCATTGTTGCCAATGG GAAGGGCATCCTGGCTGCAGATGAATCTGTGGGTACCATGGGAAACCGCCTGCAAAGGATAAAGGTGGAG AACACCGAAGAGAACCGAAGGCAGTTCCGAGAACTCCTCTTTAGTGTGGACAATTCCATTAGCCAGAGCA TCGGCGGAGTGATCCTTTTCCACGAGACCCTCTACCAGAAGGACAGCCAGGGAAATCTGTTCAGAAACGT TCTCAAGGAGAAGGGAATTGTGGTGGGCATCAAGTTGGACCAAGGAGGTGCCCCGCTTGCAGGAACAAAC AAGGAAACCACCATTCAAGGGCTTGATGGCCTCTCTGAACGCTGTGCTCAGTACAAGAAAGATGGAGTTG ACTTTGGGAAGTGGCGTGCTGTGTTGAGGATTGCTGACCAGTGTCCTTCCAGCCTTGCTATCCAAGAAAA TGCCAATGCTCTGGCTCGCTATGCCAGCATCTGTCAGCAGAATGGGCTGGTCCCTATTGTTGAGCCAGAG GTGCTTCCTGACGGAGACCATGACCTGGAGCACTGCCAGTATGTTTCTGAGAAGGTCCTGGCTGCTGTCT ACAAGGCTCTCAATGATCATCATGTTTACCTAGAAGGCACCTTGCTAAAGCCAAACATGGTGACTGCTGG ACATGCCTGCACGAAGAAGTATACACCAGAGCAGGTGGCTATGGCCACTGTCACAGCTCTCCACAGAACT GTTCCTGCAGCTGTTCCTGGTATCTGCTTTTTGTCTGGAGGTATGAGTGAGGAGGATGCTACACTTAACC TCAATGCTATCAACCGTTGCCCTCTACCAAGGCCCTGGAAACTAAGCTTTTCATATGGAAGAGCCCTCCA GGCCAGTGCATTGGCTGCCTGGGGTGGCAAGGCTGCAAACAAGAAGGCAACCCAGGAAGCTTTCATGAAG CGGGCTATGGCTAACTGCCAGGCGGCCCAAGGACAGTACGTTCACACAGGCTCTTCAGGTGCTGCTGCCA CCCAGTCGCTCTTCACAGCCTCCTACACCTATTAGATACTGTCACCACCAGCCAGGCTCCAGTTCTCCTA AATATTTTGGAGACCTGAATGGCTCAGTCAACCCTGGAAATTAGATAACATTTGATTGAACTTCTGAAAA CACAACACATGTGAATCATAAACACAAGAGAAAAGCCAAATCAGCTTTGAAACCCAAGAATGAGCATGTC ACCCAATAGTCTCTCAACACTTCCAGTTCCCTATGTTCCAGAACATTCATCCAAGAAAAGAACAGGCCAC GACTGAGAAAAGCAACTCAGAAGCAGAGGATGGAGAAGGGCATTTTAATAAACATAGTTACCAGGGGTTG GTTATAGAAGAGGCACAGCAACAGAAAAAGAAATAAAACGTGTATGAACCTTCCGTTGGTACAGTTTCAC TTTCCTGCTCTTGTTGAGGGATCCTGCATTTCATTGTCTTTGCCTATACCAGATACAACCCCAATAGTCA GGATCCTCAGCCCCAGATGCTCACTACAGTCAAGTGGCTGATGGCTCCCTTTAGCTGAATTACACTAGAA ACTCTACCAGATTAAATAAGAGTTCTTTTGGGGCTTCCTATATGCAGTCATACTTGAGAACAGTCAGTCT AGACTTCTAGCTTGAGAATATTTGAGAATACTAAGCTGCAACAGAGACTCATCTCTTCCAAGCTATGGAG TGTAGAGAATGAGTTGATAAACCTGACTTGGCATACTCCTCTCCAGGAATCCAGGTTTTGTGACACAGAA CTACATTTTCCTCTCAAAGTCAAGCTGGTCCCCCAGCTGTGCAATAAACAATCGTTGAATAATAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4975336 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4975336 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17146 same embryo
	EMAGE:17145 same embryo
	EMAGE:17147 same embryo
	EurExpress:euxassay_001529 same experiment
	MGI:4823088 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source