EMAGE mouse gene expression spatial database

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EMAGE:17190

Taf13 TAF13 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1913500)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001598. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001598_01	euxassay_001598_02	euxassay_001598_03	euxassay_001598_04

euxassay_001598_05	euxassay_001598_06	euxassay_001598_07	euxassay_001598_08	euxassay_001598_09

euxassay_001598_10	euxassay_001598_11	euxassay_001598_12	euxassay_001598_13	euxassay_001598_14

euxassay_001598_15	euxassay_001598_16	euxassay_001598_17	euxassay_001598_18	euxassay_001598_19

euxassay_001598_20	euxassay_001598_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17190_wholemount_strong.wlz

17190_wholemount_moderate.wlz

17190_wholemount_weak.wlz

17190_wholemount_possible.wlz

17190_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17190_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 14 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 07 13 14

neural retina

moderate

regional

moderate expression: see section 02 03 04 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17

tail dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4787
Entity Detected:	Taf13, TAF13 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1913500)
Sequence:	sense strand is shown >T4787 CCACGCGTCCGCCCACGCGTCCGCGAGCAGCTAGCATGGCAGATGAGGAGGAAGACCCCACCTTTGAGGA GGAGAATGAAGAAATTGGAGGAGGAGCGGAAGGTGGCCAGGGGAAGAGAAAGAGACTTTTCTCGAAAGAA TTGCGCTGTATGATGTATGGGTTTGGGGACGACCAGAATCCTTACACGGAGTCCGTGGACATTCTCGAAG ACCTTGTCATAGAGTTCATCACTGAAATGACTCACAAGGCAATGTCGATTGGAAGACAAGGTCGAGTGCA AGTTGAAGATATTGTCTTCCTAATTCGAAAGGATCCAAGGAAGTTTGCTAGAGTTAAAGACTTACTAACT ATGAATGAAGAATTGAAACGGGCTAGAAAAGCTTTTGACGAAGCCAACTATGGATCTTGACACCGTTCGT ACTTCCCGAGATTTTGGTATTTTCTGTGGAAGCCATACATTGAAGTTGTTGTCTGCAGTAACATGATAGC TCGGTATGCAATGAAAGAGGTCTTCAACTGGAGCCTCCGTTGCCTGTCTTTATCTGACCATATCTGAATT GCTTCCTGGCTTTAATAACTCCTAAGTTAAAGCATTGCAAACCATGCGGTTCCACCCGAAAGGGTGGAAG TAGTATTTATTCTTCTGGTAGACGTGTGTGTTCCAAGCTGTATACCATTGCTGCATAGCATACAATCTGA ATGTCCTTTGATTTTATAAATGGTGAGGCTGTGCTCTGTGTATCTCAAGGCTTTAGAGGACAGTGGTGGG GGAGTTATTAAAGAGAATGAGCTGGCTTGTGGGGGTTTTTAAGAAACGAAATTCTTGACTTAATAGATCA TTTGTTTTTAATATTCTTTTCTAAAGAACACTTTTTTTAAAAGAAACTAATATTTGCTAAGTGTTAGCAG TCCTCGTCTTCCTTGATGACTGTAAAGCTTCCAATACAAATGCCTCAGTAGTGGGAACGGCAGGGCTTAA AGAGCCGATGCCAGCCCAGGTGTTTCTGGCTGTGCTCTAGTGACGGAAATGTAAGTACTTTAATTGTGAG AACGGAGAGTTGATTAAATCTGTATGTGGCTGTGCAAACCATTTTCGCGGTTCAGGCTTTGTGTGTAGTT CACTTACGGTATTAAATTATTATTCTGCTTAGCTTATAATAAGGATAATTGCATTTAAAGAGGTTACTGA ATGTTACTCTGAAACTTAGTGGTGCTTTTAACATCTAAATCAGTACTTGGGGAAATACTAGCTGTTTAAT CTACTGATACAAGGAATAAAGAATGAGGAATTAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5050736 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5050736 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17188 same embryo
	EMAGE:17189 same embryo
	EurExpress:euxassay_001598 same experiment
	MGI:4828585 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source