EMAGE mouse gene expression spatial database

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EMAGE:17202

Dimt1 DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) ( MGI:1913504)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001602. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001602_01	euxassay_001602_02	euxassay_001602_03	euxassay_001602_04

euxassay_001602_05	euxassay_001602_06	euxassay_001602_07	euxassay_001602_08	euxassay_001602_09

euxassay_001602_10	euxassay_001602_11	euxassay_001602_12	euxassay_001602_13	euxassay_001602_14

euxassay_001602_15	euxassay_001602_16	euxassay_001602_17	euxassay_001602_18	euxassay_001602_19

euxassay_001602_20	euxassay_001602_21	euxassay_001602_22	euxassay_001602_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17202_wholemount_strong.wlz

17202_wholemount_moderate.wlz

17202_wholemount_weak.wlz

17202_wholemount_possible.wlz

17202_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17202_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4808
Entity Detected:	Dimt1, DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) ( MGI:1913504)
Sequence:	sense strand is shown >T4808 CACGCGGTTCCCGGCTGCTTTCGCTTGGCACAGGGACTCCTCCGAGAAGATGCCGAAGGCCAAGTCCGCA GCGAGTAGCCGCCGGCGGGATCGTCAGGAGCAGCGCCGGGAGCTGAAGCGAGCTGGAGGACTCATGTTCA ACACAGGGATCGGACAACACATTTTGAAAAATCCTCTAATTGTTAACAGCATCATCGATAAGGCTGCTTT AAGACCTACCGATGTGGTGCTGGAAGTTGGGCCTGGGACTGGCAATATGACTGTCAAGCTGTTAGAAAAG GCCAAAAAGGTAGTTGCCTGTGAACTTGATCCAAGGCTAGTAGCTGAACTTCACAAAAGAGTTCAGGGCA CGCCTCTGGCCAGCAAACTCCAGGTCCTGGTGGGAGATGTACTGAAATCAGATTTGCCATTCTTTGATGC TTGTGTGGCAAACTTGCCTTATCAGATCTCCTCTCCCTTTGTCTTCAAGTTGCTGCTCCACCGGCCATTT TTCAGATGTGCTATACTTATGTTTCAGAGAGAGTTTGCTCTTCGATTGGTTGCCAAGCCTGGAGATAAAT TATACTGTAGACTCTCCATCAACACACAGCTTTTAGCACGCGTGGACCATCTAATGAAGGTGGGGAAGAA TAACTTCAGACCGCCCCCCAAGGTGGAGTCCAGCGTGGTCAGGATAGAACCTAAGAATCCGCCACCACCA ATCAATTTTCAGGAATGGGACGGCTTAGTAAGGATCACTTTTGTTCGGAAAAACAAGACACTGTCCGCTG CATTCAAATCAAGTGCAGTACAACAACTGTTGGAAAAAAATTACAGAATTCACTGTTCAGTACAGAATAC TGTAATACCAGAAGATTTCAGTATAGCAGATAAAATACAGCAAATCCTAACCAGCACAGGTTTCAGTGAC AAACGGGCCCGTTCCATGGACATAGATGACTTCATCAGGTTGCTACATGGATTCAATGCAGAAGGCATCC ACTTCTCCTAGCCATTTGAAAACTATTTTTTCAAGACCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5053500 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5053500 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17203 same embryo
	EMAGE:17200 same embryo
	EMAGE:17201 same embryo
	EMAGE:17199 same embryo
	EurExpress:euxassay_001602 same experiment
	MGI:4824308 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source