EMAGE mouse gene expression spatial database

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EMAGE:17203

Angel1 angel homolog 1 (Drosophila) ( MGI:1915987)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001601. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001601_01	euxassay_001601_02	euxassay_001601_03	euxassay_001601_04

euxassay_001601_05	euxassay_001601_06	euxassay_001601_07	euxassay_001601_08	euxassay_001601_09

euxassay_001601_10	euxassay_001601_11	euxassay_001601_12	euxassay_001601_13	euxassay_001601_14

euxassay_001601_15	euxassay_001601_16	euxassay_001601_17	euxassay_001601_18	euxassay_001601_19

euxassay_001601_20	euxassay_001601_21	euxassay_001601_22	euxassay_001601_23	euxassay_001601_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17203_wholemount_strong.wlz

17203_wholemount_moderate.wlz

17203_wholemount_weak.wlz

17203_wholemount_possible.wlz

17203_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17203_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 14 15 16 17 18 19 20 21 22 weak expression: see section 04 05 06 07

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 08 17 18 19 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 weak expression: see section 08 14 15 16

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4801
Entity Detected:	Angel1, angel homolog 1 (Drosophila) ( MGI:1915987)
Sequence:	sense strand is shown >T4801 CCACGCGTCCGCGGACGCGTGGGGCAGAGGAGACCGGTGTGCAGCAGTGTGGAGGGGCGGCGGCGCGGGC AGGCAGCCGGCCATGATCGCGTCGTGCCTGTATTACCTGCTGCTGCCAGCCGCGCGCCTCTTCCGCTTTC TCTCAGATGCTTTCTTTACATGTCGAAAAAATGCCCTTCTGGCGAAGAGCTCATCTCCCCAGGTAGAGGG TAACTTTGCCATGGCCCCTCGGGGGCCTGACCAAGAAGAGTGTGAGGGCCTGCTGCAGCAGTGGCGAGAA GAAGGGTGGAACCAGACACCCTCAACTGCAAGCGAGGGTCCCCTTGCGGATAAGGGACTAGCTGAGAGCA GTCTGGCCCTCCTGATGGATAATTCTGGAGAACAGGATGCTGCCTCGGAGGACAAGTGGTCCAGCAGGCA GCTGAGTGACCTTCGGGCAGCAGAGAACCTGGACCAGCCTTTCCCTGAGGTGCTAGGGGAGGAGCCTCTG GCCGAGGTTGAGGGTCCTTTGTGGGCGGCTGTTCCGGTACAGACAGGGCCCCAGTATGCAGATTGTGCTG TTCTTCCAATGGGTGCGATGGCCGCAGAACAGTGGGAAGAGGACCCTGCAATGGTGGCCTGGAGCATAGC ACCGGAGCCTATGCCCCAGGAGGAGACTTCCATGTGGCCCTTTGAAGGCCTGGAACAGCTGCAGCCTCCC CCAATGGAAATACCTTATCATGAAATCCTGTGGCGAGAATGGGAGGATTTCTCCACACAGCCAGATGCTC AGGGTCTGGAGGCAGGAGACGGCCCTCAGTTCCAGTTCACTCTGATGTCGTATAATATCCTGGCCCAGGA CCTGATGCAGCAGAGCTCAGAGCTCTATCTGCATTGCCATCCAGACATCCTCAACTGGAATTACCGCTTT GCCAACCTCATGCAGGAATTCCAGCACTGGGACCCGGATATCCTGTGTCTTCAGGAAGTCCAGGAAGACC ACTACTGGGAGCAGCTGGAGCCCTCTCTGCGGATGATGGGCTTCACCTGTTTTTACAAGAGGAGGACTGG GTGTAAAACGGATGGCTGTGCTGTGTGCTACAAGCCCACCAGGTTCCGTCTGCTCTGTGCCAGCCCTGTG GAGTACTTCCGGCCTGGGTTGGAGCTCCTCAACCGGGACAATGTGGGCTTAGTGTTGCTGCTGCAGCCGC TAGTGCCAGAAGGTCTGGGGCAAGTCTCAGTGGCTCCCCTGTGTGTGGCAAATACCCATGTCCTGTACAA CCCGCGTCGGGGTGATGTCAAGCTGGCCCAGATGGCCATTCTTCTGGCCGAGGTGGACAAGGTGGCCAGG TTGTCAGATGGCAGCCACTGCCCCATCATCTTGTGTGGGGACCTGAATTCTGTCCCTGACTCACCTCTCT ACAACTTCATCAGGGATGGAGAGCTCCAGTACAATGGCATGCCAGCCTGGAAGGTTTCTGGACAGGAAGA CTTCTCTCATCAGCTTTACCAGAGGAAGCTGCAGGCCCCACTGTGGCCCAGCTCCCTGGGCATCACTGAC TGCTGTCAATATGTCACCTCCTGTCATCCCAAGAGATCAGAGAGACTGAAATATGGCCGAGACTTCCTAC TACGATTCCGCTTCTGTGACCTCGCTTGCCAGCGACCAGTGGGACTGGTTCTCATGGAAGGAGTGACAGA CACTAAGCCAGATCGACCTGCTGGTTGGGCCGAGTGTATCTTTGAGGAAGAGATATCTGAACTTGAGCCT GTCTTCCCCAGGACTATAGGCACCATCCAGCACTGCCTCCACCTGACCTCAGTGTATACCCACTTCCTGC CCCAGCATGGCTGCCCAGAGGTCACCACGATGCCCTTAGGGCTTGGGATGACAGTGGATTACATTTTCTT CTCAGCTGAATCCTGTGAGAATGAGAACAGAACCGATCACAGACTGGATCGAGATGGAACTCTCAAGCTC CTGGGCCGCCTCTCCCTCCTCTCTGAAGAGATTCTCTGGGCTGCCAATGGCTTACCCAACCCCTTCTACT CTTCAGACCATCTCTGCTTGCTTGCCAGCTTCGGCATGGAAGTCACTGCCCCATGAGGGGCCCCAGGGAA AGAGCTCACTGGACCGACGACTGCAAATGCCCCGTGCTGTGGAAACTTCCGTCCCTTCCCTCGTCCCCTC CTGCCCGTGGTTAGACTTTCTCCAGGCCTGTCCACGTTCTTGGCCTGTGGTCCCTGCCCTGCCCCAACCT CTTCCTAATCCTGGGCCACATGCTAGGTGGCCCCTCGAGAGGTGGAGAATACTCCCCTTTCTTCCATGTA CCCAGCACCCCTCCCCCCATTGATTTTTTTTAATGACCAGGGTTGCGGGAGTTATTGATCTCATTGGTTA TTCGCTTTCAGGCCATTTCTTGGTGGCACCCTCTGACCCAACCTTCCCTTTCCTTTCATGACCTCTGGGC TCTGGCATGCACATGAGCAAGGTGTGTGTAAAGCGTGAGTTCTCTTGGAGCGGGACTGCCCAGCACAGCC AAAGCCTACCTCTGCCTAGTCCATGTGCTACCTATCATGAGCTGCTGGGCTAGACACAGGTTGAAAGCAT GACATAGTAGCTGTGTGCTGCCGGGAAGCAGCAGGTGTGGGGCCCTTCTCTCCCAGTCAGTCATCTCAGC ATCCTGGGCACACTTGTTATTTCTGGAGACAAATTGGCCCCCAGCCTGGAGCAAGCAGCTCAGGGATGCC CTAACCTCAGATCTCCTCACTGCCTTCCTTGAGGAAAGCGGGTGGATGCCTTGTGCTGGGGTTCACTGGG CTGGCACAGTGCTGGGAGGCTATGGCATTTGAAGGGTGAGGAGACTGAGCCTTCAGGGAACTGTTGTCTC ACCAGACTCTGAAGAACTGGGTAAGAGTTGGAGTCCCAGCAGAAAGAGCAGGAGGCTCTACAGCAGCCTT GGCCAGCACTGGAGCCCCAGAGGGCTTGATCTCTGACCTCTGTGTACCTAAGAAAGTTTCTTTTCTTCTT CTCTCTCCTCCTCCTCCCCTCCTTTGTTCCCTCCCATTTTCTCTCTGGAGAAGCCCGCTGTTTAGCTCAG GCCAAAACATCTCAACAGAAGCCTGGCTTGGGGCAAACTAGGTCTAAGAACTCAGGGTGGCAGTGCTTGC CCATGTGCTCTGCCTTTTCCTCCCCAAACATGGGCTCCGCCCGCTCCCCTAACCTACTGAAAACCCCTGC AGCCCAGACCGTGTCACCTGCATGCCTTACTCACTGCTGGGGTGCAGCCTAGGGTGACCTCAGGCCAGAG CCTCCACATAGCTTCAACTCAGAAGATCTGCTGGCCCAGAGATGAGGGGATTGACTGAGGCTGCAGTTAG GCATGGTGGAGCAGCAGTTACACCCTTCCCTTGAGTTGAAAGCCAGAGACCTGTCTGGAATGCTGCTGCT GCTGCTGCTGCTGCTGCTGCTGCGTTGCTGACTCTCAGCTTCCCATGCTCAGGTCTGGGGGACGTCTTTT CTTATGTGTGTCTTATTTTTAAGTTTTGTTATCTGGGTACTGGACATCTGCCTGTCTGAGCCCCAGGCCC ATCTGTCTGTCCACAGCCCACTTTTCACCGTCACTCTCTGGGCACCTCCTGAGATGGTTCCACAACCGTG CCCTGTTGGTTCTCAGAACAGGGGCCAATGATTGTTCCCCCTGCCAGGAAATGCAGGTTCAACTGCTAAA GGAGGCAGAGGGTCAGAGCTTGGTGGTTTATCTTTTCCTTGTTTCTCCTTGTTTCTGTTTTAGAAATGAA GTGTGGGGGTTTAAATGGCACTTAAACTGCACCCTGGAGCCGGCAGAACCAGGCAACGTTTTTCCAAATA AAGGCTTACCATGCCCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5052974 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5052974 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17202 same embryo
	EMAGE:17200 same embryo
	EMAGE:17201 same embryo
	EMAGE:17199 same embryo
	EurExpress:euxassay_001601 same experiment
	MGI:4823121 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source