EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17209

Lect2 leukocyte cell-derived chemotaxin 2 ( MGI:1278342)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001610. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001610_01	euxassay_001610_02	euxassay_001610_03	euxassay_001610_04

euxassay_001610_05	euxassay_001610_06	euxassay_001610_07	euxassay_001610_08	euxassay_001610_09

euxassay_001610_10	euxassay_001610_11	euxassay_001610_12	euxassay_001610_13	euxassay_001610_14

euxassay_001610_15	euxassay_001610_16	euxassay_001610_17	euxassay_001610_18	euxassay_001610_19

euxassay_001610_20	euxassay_001610_21	euxassay_001610_22	euxassay_001610_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17209_wholemount_strong.wlz

17209_wholemount_moderate.wlz

17209_wholemount_weak.wlz

17209_wholemount_possible.wlz

17209_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17209_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

weak

regional

weak expression: see section 01 02 03 04 22

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

lung

moderate

regional

moderate expression: see section 04 09 12 17 18 21 weak expression: see section 05 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4782
Entity Detected:	Lect2, leukocyte cell-derived chemotaxin 2 ( MGI:1278342)
Sequence:	sense strand is shown >T4782 CCACGCGTCCGCTTGCTTTCTGTGTCTCTTACTAGATCTCGGAGAGACATAAATTACCAGAGAAGATTCA CTCATTCCGGGGGTGACCCTGTGTGCATGGAGCAGGTCTAACTAGAGGAAGAAACAAGAAGCAGAACCTA GATGATTCCCACAACAATCCTCATTTCAGCTGCTTTGCTTTCCTCTGCCCTAGCAGGACCATGGGCTAAC ATATGTGCCAGCAAATCTTCCAACGAGATCCGGACGTGTGACAGCTATGGCTGTGGACAGTACTCTGCTC AAAGAACCCAAAGGCATCACCCAGGTGTGGACGTCCTGTGCTCGGATGGATCTGTGGTGTATGCACCATT CACTGGGAAGATAGTGGGCCAGGAGAAACCCTATAGAAACAAAAATGCCATCAATGATGGCATTCGACTG TCTGGAAGAGGTTTTTGTTTCAAAATTTTCTACATTAAGCCAATTAAGTATAAAGGTTCTATCAAAAAGG GGGAGAAGCTGGGCACCTTGCTGCCCCTGCAGAAAGTTTACCCGGGCATCCAGTCGCATGTACACGTTGA AAACTGCGACTCCAGTGACCCCACAGCATACCTGTAAGCAGAGACAAAGGCCAGATCTTCTAAATTCAAA GCCATCTCAGAAACTGGGACATGCCCTGCTCCCGAAGAAACGTGCATTCTAACAGAAACATAAATCTGTG TAAACACTCACAACCCTGATTCCAAGTCATCACCGCTCTGACGGGTGGGCTCTGGTCGGCCTGCCACCTC AAGGGCCAGGGAGTTTGACATTTTCGATTTTTAGGTTCTGGTGACTGAGATAAATGAATGACCTCTCAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5049578 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5049578 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17208 same embryo
	EMAGE:17207 same embryo
	EurExpress:euxassay_001610 same experiment
	MGI:4825897 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source