EMAGE mouse gene expression spatial database

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EMAGE:17222

Slmap sarcolemma associated protein ( MGI:1933549)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008883. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008883_01	euxassay_008883_02	euxassay_008883_03	euxassay_008883_04

euxassay_008883_05	euxassay_008883_06	euxassay_008883_07	euxassay_008883_08	euxassay_008883_09

euxassay_008883_10	euxassay_008883_11	euxassay_008883_12	euxassay_008883_13	euxassay_008883_14

euxassay_008883_15	euxassay_008883_16	euxassay_008883_17	euxassay_008883_18	euxassay_008883_19

euxassay_008883_20	euxassay_008883_21	euxassay_008883_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17222_wholemount_strong.wlz

17222_wholemount_moderate.wlz

17222_wholemount_weak.wlz

17222_wholemount_possible.wlz

17222_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17222_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4826
Entity Detected:	Slmap, sarcolemma associated protein ( MGI:1933549)
Sequence:	sense strand is shown >T4826 CCACGCGTCCGCCCACGCGTCCGAACAGAAGGGACAAGCTGAGAAGAAGGAATTACAGACGAAAATTGAT GAAATGGAAGAAAAAGAACAAGAGCTCCAGGCAAAAATCGAAGCTTTGCAAGCTGATAACGACTTCACCA ATGAAAGGCTGACAGCTCTGCAAGTACGGTTAGAACACCTGCAGGAGAAAACCCTTAAAGAATGCAGCAG CTTAGAGAAGCTGATGGTCCAAGGGCATCTAACCAAAGTGGTAGAAGAATCAAAGCTTTCAAAAGAAAAT CAGGCAAAAGCAAAGGAATCCGACTTATCAGACACTCTGAGTCCAAGCAAGGAAAAAAGCAGCGACGACA CTACAGACGCCCAGATGGATGAGCAAGACCTAAATGAACCCCTTGCTAAGGTGTCTCTATTAAAAGATGA CTTACAGGGTACCCAGTCAGAAACTGAGGCCAAACAAGACATTCAGCATCTTCGCAAAGAATTGGTGGAA GCCCAGGAGCTAGCTAGAACAAGTAAACAAAAATGCTTTGAACTTCAAGCTCTTTTGGAAGAAGAACGAA AAGCCTATCGAAATCAAGTTGAAGAATCAGCTAAACAAATACAGGTTCTTCAAGTCCAGCTGCAGAAGTT ACACATGGACATGGAGAATCTCCAGGAGGAAAAGGACACGGAAATCTCCAGCACAAGAGATAAATTGCTT AGTGCCCAAGATGAGATTTTGCTCCTTCGTCAAGCAGCAGCAGAGGCTGTGTCTGAGCGGGACACTGACT TTGTTTCTTTGCAAGAAGAGCTTAAGAAGGTCAGAGCGGAGCTTGAGGGCTGGAGGAAAGCAGCGTCTGA ATACGAGAATGAAATCAGAAGTCTGCAGAGCAGCTTCCAGCTTAGATGTCAGCAGTGTGAAGACCAGCAA AGGGAGGAGGCAACAAGGTTGCAAGGTGAGCTGGAGAAGTTGAAGAAGGAGTGGGATGTACTGGAAACGG AATGCCATTCTCTAAAAAAGGAGAACGTGTTGTTGTCTTCAGAACTGCAGCGACAAGAGAAAGAATTGCA CAATTCTCAGAAGCAGAGTTTTGAACTCACGAGTGACCTCAGCATCCTTCAGATGACTAGGAAAGAGCTG GAGAAGCAAGTGGGATCCTTGAAAGAACAACACCTTCGGGATGCAGCTGACCTAAAAACTCTCCTCAGTA AGGCTGAAAACCAAGCAAAGGATGTACAGAAAGAGTATGAAAAGACACAGACTGTTCTCTCAGAACTGAA GTTGAAGTTTGAAATGACTGAGCAGGAAAAGCAATCAATCACAGATGAGCTCAAACAGTGTAAAGACAAC CTGAAGCTGCTGCGAGAGAAAGGAAATAATAAACCCTGGCCCTGGATGCCCATGTTGGCGGCCCTGGTTG CGGTGACAGCTATGGTGCTGTATGTACCAGGTCTGGCCAGAGCTTCTCCATGACAGTGTGTCTTGAGTCT GTACACCGTCCTCCCTCTAGAAGCCGGCAGCACACTCATGCTGGGGACAAGCAGAACCATTCTCTTCCTT TTTACCTCTTAAAACAGCAAAGTGCAAGAATACAGCTGGAGGGTCATTGCTTTAATTTATATAAAATATA TCTGTCTATAGAGAGGATAGAAAGTTGTGAATTTATTCGGTTTTACATGTAATTTTTAAATTAGTATGTT GAGATTCTGAATATTATGGTGGCCTAAAGTACGCTTCTTAGTGCACCAAATTATTTATACCATAAATTTA TAGATATTTTACTCTGAATTCTAATGGCCACACAATTCATTTTTCTGATTCGATAACTACTCAAACCGAA CAACCAATACATACATGGGAATAGAGGCCCTGTGTGCTCAGTGCCTATCAGTTTGAATATATACACACAC ACATATATATATTTTTTTTACATATTTACGCCATTTTTACTTGTTATAAAACCACTGAGCTATTGGGAAC AAGACTTAGACAATTATTTGTGTGGATTTTTTTTTTAAAGGAAAACTACATTTGAAAAATATTCTCTTAT AATAATTATTTGGGGAATACATACGTGTTTGTTCAGCCTTAATGTGACTTGAAGATGTGAAGGACATCAG CTTTGAAAACTTTCCATGAGAGTTTGTATTTTCTTGAGCAAACTGAAATATTACTGGGAGCAAAAACATA AGTGCATAATTTCAATGCAATCAACCAAACTGTTGAGTGAATTGGAATGTAACTTACTAAACCTCATGTA TTTAAGAAATGTTTTCTTTAAAAGCCAAGTTACCTTCTCATATACAGCAGTTTAGAAAGCATGATTTTTT TTTCTGTCATCTGTTCCTCCAAGCATGTTTAATTGAAAGAAGAATTATATCTGTTTAGATAAGCTTTTAT TGACTTAATTTGGTTATGATGTGGAGCTAATTCACATTCAAGGTGAACTGATGTTCTCTGCTAGCAGATT TCTTGGTTGGGTATACAAATGGTTATTCTCTTTTTGTTGTTAATTGGAACAGCCTGTCAATTAGTAGCAC TATTCCCAAGATTGGCATTGTTCCACACACAGTGACACACCTCAGCACTGCTTGGCATTATACATTTAAA CACAGAGGTTAAAGTTTAGCTGTGGTTTTGTGCAGCACCATAGTGATGTCAATGTCCTAAAGTATCACGT GCTTAGGTTTTCTTCAGTCAGGGAAGACTGGAAGGGAAGGCCAGTGAGTTGGTTTGAATGTTGTATGAGA ACACCCAGATCTAGCCAGATATTGAAAAAGGACTTTAGGATATAATTTGTCTGTGATGTTTGGCAGTCAT TATTTTTACTTTAAAAGTTGTATTTAAAGCTATTTGGGATTGTTGCCGGGAGAATCACTATATTCATGGA GGAATTAGCCACAAATGACTTGTAGAAAATACTGTCGTATAGTTCATTTTACCATTCTCTGTTGCAGGAA GCCACCCCACCACAGAATGCTGATATGCCAGTGGTACCCAGTACCTCTTGTATATAGTTATTGCAAATAC TGTTATGAAATGCATAACTTCAAAATTACCTTTTTTAAAGTAAATAATTGTTTGAAATCTATTTTGTAAA GATAAAACGTACAATAGAATTTCTGGAGTACAGATTAAACTATTTGCACTAACACACGTGATGTGCATGA TTTAATAAAATAACCTTAATCTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5064534 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5064534 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17223 same embryo
	EMAGE:17226 same embryo
	EMAGE:17221 same embryo
	EMAGE:17224 same embryo
	EMAGE:17225 same embryo
	EurExpress:euxassay_008883 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source