EMAGE mouse gene expression spatial database

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EMAGE:17224

Hoxd9 homeobox D9 ( MGI:96210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008880. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008880_01	euxassay_008880_02	euxassay_008880_03	euxassay_008880_04

euxassay_008880_05	euxassay_008880_06	euxassay_008880_07	euxassay_008880_08	euxassay_008880_09

euxassay_008880_10	euxassay_008880_11	euxassay_008880_12	euxassay_008880_13	euxassay_008880_14

euxassay_008880_15	euxassay_008880_16	euxassay_008880_17	euxassay_008880_18	euxassay_008880_19

euxassay_008880_20	euxassay_008880_21	euxassay_008880_22	euxassay_008880_23	euxassay_008880_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17224_wholemount_strong.wlz

17224_wholemount_moderate.wlz

17224_wholemount_weak.wlz

17224_wholemount_possible.wlz

17224_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17224_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forearm

strong

regional

strong expression: see section 01 23 24

hand

strong

regional

strong expression: see section 02 03 04 21 22 23

foot

strong

regional

strong expression: see section 06 07 08 18 19 20 21

upper leg

strong

regional

strong expression: see section 02 24

spinal cord

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

midgut

strong

regional

strong expression: see section 06 07 08 09 10 11 12

metanephros

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18 19

testis

strong

regional

strong expression: see section 04 05 06 07 08 18 19 20

clavicle

strong

regional

strong expression: see section 02 03 04 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4823
Entity Detected:	Hoxd9, homeobox D9 ( MGI:96210)
Sequence:	sense strand is shown >T4823 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGCCGCCGCCACCAGGGCGGGCTGAGCCGGGCCAGG CCGGGCTGGTGCGTGGACCTGGGCCCGGCAGCTCTAATTGCAACGCTCCTGTTGATGATGATTTTTTTTT TTTTAAATCACAGCAGCCCCCAGTTTAGCGGACTGATTTACTCCGGGTATTGGTAAATATGATCACGTGG GCCGCGCGACCAATGGTGGTGGCTGCAGCCTGCGAACTAGTCGGCGGCTCGGGCGCCGGCGAGGAGCCGC TCGGCGGTGGGCAGTGGAACCTTGGGTGGGAGCGAGCGGCGCCTCACCATGTCGTCCAGTGGCACCCTCA GCAACTACTACGTGGACTCGCTCATAGGCCATGAGGGCGACGAGGTGTTCGCCGCGCGCTTTGGGCCACC GGGGCCGGGGACACAGGGCCGGCCCGCAGGTGTGGCCGATGGCCCAGCCGCCGCCACCGCCGAGTTCGCC TCGTGTAGTTTTGCCCCCAAATCGTCCGTGTTCTCCGCCTCGTGGTCCGCGGTGGCCGCCCAGCCCCCGG CGGCGGCGACGATGAGCGGCCTGTACCACCCGTACGTGTCCCCGCCGCCCCTGGCTGCCGCCGAGCCGGG ACGCTACGTACGCTCCTGGATGGAGCCGCTGCCCGGCTTCCCCGGCGGCGCAGGCGGTGGCGGTGGCAGT GGTGGCGGCGGTGGCGGAGGCCCGGGTCCCGTTCCCAGCCCCGGTGGCCCAGCCAACGGGCGCCACTACG GGATTAAGCCTGAAACCGGAGCGGCCCCGGCCCCCGCTGCAGCCTCCACCTCCTCCTCCTCCTCCACCTC CTCGTCCTCCTCCTCCAAAAGGACTGAGTGCTCCGCGGCCCGCGAGTCCCAGGGGAGCGGCGGCCCAGAA TTCCCGTGCAACTCGTTCCTGCGGGACAAGGCAGCGGCGGCGACAGGGAACGGGCCCGGGGTGGGAATCG GGACCGGGCCAGGGGCGGTAGGCTCGTCCGAGCCCTCAGCTTGCAGCGATCACCCGAGCCCGGGCTGCTC GCTGAAGGAGGAGGAGAAGCAGCCGCCGCAGCCGCCGCAGCAGCAACTTGACCCAAACAACCCTGCAGCG AACTGGATCCACGCTCGCTCCACCCGGAAAAAGCGCTGTCCCTACACCAAATACCAGACGCTAGAGCTGG AGAAGGAATTCCTCTTCAACATGTACCTCACCCGGGACCGGCGCTACGAGGTGGCCAGGATTCTGAACCT TACAGAGAGACAAGTCAAAATCTGGTTCCAGAACCGTAGGATGAAAATGAAAAAGATGAGCAAGGAGAAG TGCCCTAAAGGAGACTGACCCAGTGCGGGTCTGGCCTGCAGCACTCAAAAGGCAGCGGGTTTTTTTGTTT GTTTGTTTGTTTGTTTTGTTTTTTGTTTTTTGTTGTTGTTTGTTTTCTTTGTGGGTGCTTGATTCAGAAA CTCTGCAGCGACTCGGACTTTCTTCTTTTTTTAGGTAGAAGTGACTGTGTGGTTGGTCTCTGTGAGTTAT CTGGGGGACACTGTATTTGCTCGCATATGTATTGGAGAAACCAAGTGGCTTTGGAGTCTTGTCCTGTCCC ACTCCCTCCCTTCCCTGCTCTCCTGATTCCTTAGGAAATGCTATATTTTGTGAGTGCACGCAGGCTTGAT GGAGCCCTATTCTATGTAAATGTCCCTCATGTTTCTGAAAAGTGCTGTAGTTTAGTCTCACCCTATCCAG CCTCCCAAACAGGGCCAACGATCTAATTCCGAGAATCTGAAGGAAGACAGAGTGTGGACACCCCCACCCT GCTCTCCCAACTAAAATCCAACTCAGCTGCTCAAGGCAGGAAAACCCCTTTCCTCGGGGAGCCGGCGGAC TGACTTCCACAGAGGCCTACCGAGAAACCTTGGGCCCTGGGGCGCGAGATTGGGGGTGGGGTGGGGATAG GCTTAGTCCTCAGAGCACCGGGGAAGCTACTTTCTCCCCGGGAGGGCTGAGCTCCCATTGGGCGGCCTCG GGAGGTTGCCTTCCATATTCCTGCCTGAGGACCAGTTGTAAATGTTACCGCTTCCAACAAATAAATGCTG ATCTGAACAAATGGAGCCCAGCCGCTGGCCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5064578 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5064578 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17223 same embryo
	EMAGE:17226 same embryo
	EMAGE:17221 same embryo
	EMAGE:17225 same embryo
	EMAGE:17222 same embryo
	EurExpress:euxassay_008880 same experiment
	MGI:4825439 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source