EMAGE mouse gene expression spatial database

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EMAGE:17233

Map3k12 mitogen-activated protein kinase kinase kinase 12 ( MGI:1346881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008881. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008881_01	euxassay_008881_02	euxassay_008881_03	euxassay_008881_04

euxassay_008881_05	euxassay_008881_06	euxassay_008881_07	euxassay_008881_08	euxassay_008881_09

euxassay_008881_10	euxassay_008881_11	euxassay_008881_12	euxassay_008881_13	euxassay_008881_14

euxassay_008881_15	euxassay_008881_16	euxassay_008881_17	euxassay_008881_18	euxassay_008881_19

euxassay_008881_20	euxassay_008881_21	euxassay_008881_22	euxassay_008881_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17233_wholemount_strong.wlz

17233_wholemount_moderate.wlz

17233_wholemount_weak.wlz

17233_wholemount_possible.wlz

17233_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17233_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5642
Entity Detected:	Map3k12, mitogen-activated protein kinase kinase kinase 12 ( MGI:1346881)
Sequence:	sense strand is shown >T5642 GAGCCCCCGAGGGCCCGCTGCTCAGCACACCTGGCTCCAGAGGCCATGGCCTGCCTCCATGAAACCCGAA CACCCTCCCCTTCCTTTGGGGGCTTTGTGTCTACTCTAAGTGAGGCTTCTATGCGAAAGCTGGATCCAGA CACTTCAGACTGCACCCCCGAGAAAGACCTGACGCCCACCCAATGTGTACTTCGAGATGTTGTGCCTCTC GGTGGGCAGGGTGGAGGAGGACCCAGCCCCTCCCCAGGTGGAGAGCCTCCCCCAGAGCCTTTTGCCAATA GTGTCCTCCAGCTACATGAGCAGGATACAGGTGGGCCAGGGGGAGCCACTGGGTCACCTGAGAGTCGAGC ATCTAGAGTTCGAGCTGATGAGGTACGTCTGCAGTGCCAGAGTGGCAGTGGCTTCCTTGAAGGTCTTTTT GGCTGCCTGCGCCCTGTCTGGACAATGATTGGCAAAGCCTACTCCACAGAACACAAGCAACAGCAGGAAG ACCTTTGGGAAGTCCCCTTTGAGGAAATCCTGGACCTGCAGTGGGTAGGCTCAGGGGCTCAGGGTGCTGT TTTCCTGGGACGCTTCCACGGGGAGGAGGTAGCTGTGAAGAAGGTTCGAGATCTCAAGGAGACTGACATC AAGCATCTTCGAAAGCTGAAGCACCCCAACATCATCACTTTCAAGGGTGTTTGCACACAGGCCCCCTGCT ACTGCATCCTTATGGAATTCTGCGCTCAAGGACAGCTATATGAGGTGCTCAGAGCCGGCCGCCCTGTCAC CCCCTCCTTGCTGGTTGACTGGTCCATGGGCATCGCTGGTGGCATGAATTACCTGCACCTGCACAAGATT ATCCACAGAGACCTGAAGTCACCCAACATGCTAATCACATACGACGATGTGGTGAAGATCTCAGATTTTG GCACTTCCAAGGAGCTGAGTGACAAGAGCACCAAGATGTCCTTTGCAGGAACAGTAGCCTGGATGGCTCC TGAAGTGATCAGAAATGAACCTGTGTCTGAGAAGGTTGACATCTGGTCCTTTGGGGTGGTGCTATGGGAA CTACTGACTGGAGAGATTCCCTACAAAGATGTAGATTCCTCAGCCATCATCTGGGGTGTGGGAAGCAACA GTCTCCACCTGCCTGTACCCTCCAGCTGCCCAGATGGTTTTAAAATTCTACTTCGCCAGTGCTGGAACAG CAAACCACGAAATCGCCCATCATTCCGACAGATCTTGCTGCACCTGGACATCGCCTCCGCTGATGTGCTC TCTACACCCCAGGAGACTTACTTTAAGTCCCAGGCAGAGTGGCGGGAAGAAGTAAAACTGCACTTTGAAA AGATTAAGTCAGAAGGGACCTGTCTGCACCGCCTAGAAGAGGAACTGGTGATGCGGAGAAGGGAGGAGCT CAGACATGCCCTGGACATCAGGGAGCACTATGAACGGAAGTTGGAGAGAGCCAACAACCTGTACATGGAA CTGAATGCCCTCATGCTGCAACTAGAACTCAAAGAGAGGGAATTGCTCAGGCGAGAGCAGGCTTTAGAAA GGCGGTGTCCTGGTCTACTAAAGTCACACCCTTCTCGGGGCCTCCTACATGGAAACACTATGGAGAAGCT CATCAAGAAAAGGAACGTGCCACAGAAACTGTCGCCCCACAGCAAAAGGCCAGATATTCTCAAGACAGAG TCGTTGCTACCTAAACTAGATGCAGCCCTAAGTGGGGTGGGGCTTCCTGGGTGTCCTAAGGGCCCCCCTT CACCTGGAAGGAGTCGGCGTGGCAAGACCCGTCACCGAAAGGCCAGTGCCAAGGGCAGCTGTGGAGACCT GCCTGGGCTTCGTGCAGCTTTGCCACCCCATGAGCCTGGAGGACTAGGAAGCCCAGGGGGCCTAGGAGTG GGCCCTTCAGCTTGGGATGCTTGCCCCCCTGCTCTCCGTGGACTCCACCATGACCTTCTACTCCGAAAGA TGTCATCATCATCCCCAGACCTGCTATCAGCAGCGCTGGGAGCCCGAGGCCGAGGGGCTACAGGGGGAGC TCGGGATCCTGGCTCACCACCTCCACCCCAGGGCGATACTCCTCCAAGTGAGGGATCAGCTCCTGGTTCC ACCAGCCCAGATTCGCCTGGGGGAGCTAAAGGGGAACCCCCTCCACCAGTAGGGCCTGGAGAAGGTGTGG GGCTGCTGGGAACTGGAAGGGAAGGGACTGCAGGCCGGGGAGGAAACCGGGCTGGGTCCCAGCACTTGAC CCCCGCTGCGCTGCTGTACAGGGCTGCTGTGACTCGAAGTCAGAAACGTGGTATCTCATCTGAAGAAGAA GAAGGAGAGGTAGACAGTGAAGTAGAGCTACCCCCAAGTCAAAGGTGGCCTCAGGGCCCGAACATGCGTC AGTCACTATCTACATTCAGCTCAGAGAACCCATCAGATGTGGAGGAAGGTACAGCTAGTGAGCCTTCCCC AAGTGGCACACCAGAAGTTGGCAGTACCAACACTGATGAGCGACCAGATGAACGATCTGATGACATGTGC TCACAGGGCTCAGAAATTCCACTGGACCTACCTACTTCAGAGGTGGTCCCTGAACGTGAAGCCAGCTCCT TGCCCATGCAACACCAGGATGGCCAGGGCCCCAATCCTGAAGACTCAGACTGTGACAGCACTGAATTGGA CAACTCCAACAGCATTGATGCCTTGCGGCCCCCAGCCTCCCTTCCTCCATGAAAGACACTCTTATTCCTT GTACATAGAGAAATATTTATATAAATAATATATATGCGCCACATAATCAACAGATAGATGGGGCTTTCTT AGCCTTAAGCCAGGCTTGAGAGTGAACGACGCCCCTGACCAATTACCGAATAAGTTCTGGGGACACTGGC AGCTGTGGAAATGAATGACTCCGAGTACTACCCTAGAGCTATAAAGAAAGGAAGCTGGCCCCTTCTTGCT TCACCCCATTCCTTATGTTGAACAAGCAAGTAGGCAATAGAAAAGCCAGGCTTGTCTCTATACTGTCTAT CCCTGAACACTGGAGGGGCAGGTAGAGGATTCACTTAAAACTGCACTTTTATTTTCCGTTTACTGTTTAC ACATTTTGCACTTGGGAGGAGGGAAAGAAACTAAGGCTGGGTCATCCCTCTGAGGTTTCTCAGGTGGCAA TGTAACTCATTTCTTTGTTCCTGTTTCTCTGCCCAAACCCTGGCTTTGGGCCAGACGACTAACAGCATGT GATGGCTCAGGCTGAAGAACTGGGGTGCTGTTAGTCCCCACTGTTATCTTGGTGCCTGGTAGGGGTGGGG ACTGTCATATTGTAACCCCTGTGAACAACCTTGAAATATAACCACTCCATGCAGGCCCAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6494216 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6494216 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17234 same embryo
	EMAGE:17237 same embryo
	EMAGE:17236 same embryo
	EMAGE:17232 same embryo
	EMAGE:17235 same embryo
	EurExpress:euxassay_008881 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source