EMAGE mouse gene expression spatial database

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EMAGE:17236

Mgea5 meningioma expressed antigen 5 (hyaluronidase) ( MGI:1932139)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001624_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17236_wholemount_strong.wlz

17236_wholemount_moderate.wlz

17236_wholemount_weak.wlz

17236_wholemount_possible.wlz

17236_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17236_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5644
Entity Detected:	Mgea5, meningioma expressed antigen 5 (hyaluronidase) ( MGI:1932139)
Sequence:	sense strand is shown >T5644 CCGGGCACTCCGGGCCCCCGCCGAGAGGAAGGGGGAAGGGCGGAGGGGGAGGGGAAGGAGCCCCGGAGGC GCGCGTTCGGAGCCGCGGCCCCTTTGCAGGGCTCCGGGCCTTTGGCCCAACGGACCGAGGCCGAAGGGGA GCGGTGGCCGGCGGCAGGAGGATGGTGCAGAAGGAGAGCCAGGCGGCGCTGGAGGAGCGGGAGAGCGAGC GCAACGCCAACCCTGCCGCCGCCTCGGGGGCTTCGTTGGAGCAGTCGGTAGCTCCCGCCCCCGGAGAAGA TAACCCATCGGGAGCCGGGGCTGCGGCCGTGGTCGGGGCGGCCGGAGGGGCCCGCAGGTTCCTGTGCGGT GTCGTGGAAGGGTTTTATGGAAGACCTTGGGTTATGGAGCAGAGAAAAGAGCTCTTTAGAAGGCTTCAGA AATGGGAATTAAACACATACTTGTATGCTCCAAAAGATGACTACAAACATAGGATGTTTTGGCGAGAGAT GTATTCAGTGGAAGAAGCTGAGCAACTTATGACTCTGATCTCTGCTGCACGAGAATATGAAATAGAATTC ATCTATGCTATTTCGCCAGGACTAGATATCACTTTTTCTAACCCCAAGGAAGTATCAACGTTGAAACGCA AATTGGACCAGGTCTCTCAGTTTGGGTGCAGGTCGTTTGCTTTGCTTTTCGATGATATTGACCATAATAT GTGTGCAGCAGACAAAGAGGTGTTCAGTTCTTTTGCACATGCACAAGTTTCCATCACCAATGAAATTTAT CAGTACCTGGGAGAGCCAGAAACCTTCCTCTTCTGCCCTACAGAATACTGTGGCACTTTCTGTTATCCAA ATGTATCTCAGTCTCCTTACTTGAGGACTGTGGGTGAGAAGCTTCTACCTGGAATTGAGGTGCTTTGGAC AGGTCCAAAAGTTGTTTCAAAAGAAATTCCAGTGGAATCTATTGAAGAAGTTTCTAAGATCATTAAGAGA GCTCCAGTGATCTGGGATAACATTCATGCTAATGATTATGATCAGAAGAGGCTGTTTCTGGGTCCCTACA AAGGAAGATCCACAGAGCTCATCCCACGGTTAAAGGGAGTACTAACCAATCCAAATTGTGAATTTGAAGC TAACTACGTTGCCATACACACCCTTGCCACTTGGTACAAGTCAAACATGAACGGAGTGAGGAAGGATGTG GTGATGACTGACAGTGAAGACAGTACTGTATCAATACAGATAAAGTTAGAAAATGAAGGCAGTGACGAAG ACATTGAGACTGATGTTCTCTACAGCCCACAGATGGCTTTAAAGCTGGCTTTAACTGAGTGGCTGCAGGA GTTCGGTGTCCCTCATCAGTACAGCAGTAGGCAAGTTGCACACAGTGGAGCTAAGACAAGTGTAGTTGAT GGGACTCCCTTAGTTGCTGCACCCTCTTTAAATGCCACAACTGTAGTTACAACAGTTTACCAGGAGCCCA TTATGAGCCAGGGAGCAGCTTTGAGTGGTGAACCCTCAGTTCTGACCAAGGAAGAGGAAAAGAAACAGCC AGATGAGGAACCCATGGATATGGTGGTCGAAAAGCAAGAAGAAGCAGAGCACAAGAATGACAACCAGATA CTAACTGAGATTGTTGAAGCGAAGATGGCAGAGGAGTTGAGACCAATGGACACGGATAAGGAGAGCATGG CTGAGTCCAAATCCCCAGAGATGTCCATGCAGGAAGACTGCATTCCTGACGTGGCTCCTATGCAGACAGA TGAGCAAACACAGAAGGAGCAGTTTGTGCCAGGTCCAAATGAAAAGCCTCTGTACACCGCAGAGCCTGTG ACTCTGGAGGACTTACAGTTACTCGCTGACCTGTTCTACCTTCCTTACGAGCACGGGCCCAAAGGAGCGC AGATGCTACGGGAATTCCAGTGGCTTCGAGCAAACAGCAGTGTTGTCAGTGTCAACTGCAAAGGGAAAGA CTCAGAAAAAATAGAAGAATGGCGGTCACGAGCAGCCAAGTTTGAAGAGATGTGTGCACTAGTGATGGGG ATGTTCACCCGGCTTTCCAACTGTGCCAACAGGACCATCCTTTATGACATGTACTCCTATGTGTGGGACA TCAAGAGTATAATGTCTATGGTGAAATCTTTTGTGCAGTGGTTAGGGTGTCGTAGTCACTCTTCAGCACA GTTCTTAATTGGAGACCAGGAGCCCTGGGCCTTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTG CCAATTGATGGAGCAAATGATCTCTTTTTTCAACCACCCCCACTGACTCCAACCTCCAAAGTTTATACTA TCAGACCTTATTTCCCTAAAGATGAGGCTTCCGTGTACAAGATTTGCAGAGAAATGTATGATGATGGAGT GGGTTTCCCTTTTCAAAGTCAACCTGACCTTATTGGAGACAAGTTAGTAGGAGGACTGCTCTCCCTCAGC CTGGATTACTGCTTCGTCCTAGAAGATGAAGATGGCATTTGTGGTTATGCCTTAGGCACAGTAGATGTGA CCCCCTTCATTAAAAAGTGTAAAATTTCCTGGATTCCCTTCATGCAAGAGAAGTATACCAAGCCAAATGG TGACAAAGAGCTCTCAGAGGCTGAGAAAATAATGTTGAGTTTCCATGAAGAGCAGGAGGTGTTGCCAGAA ACTTTCCTTGCCAACTTCCCTTCTCTGATAAAAATGGATATTCATAAAAAAGTGACTGACCCGAGTGTTG CCAAAAGCATGATGGCTTGCCTCCTGTCTTCACTGAAGGCCAATGGCTCCCGGGGGGCTTTCTGTGAAGT AAGACCAGATGATAAAAGGATTCTGGAATTTTACAGCAAGTTAGGCTGTTTTGAAATTGCAAAAATGGAA GGATTTCCAAAGGATGTGGTTATACTTGGTCGAAGCCTGTGACACTTGTTGGTACTGTGAACTGTCCAAA AGTCCCTTAACTCCGCCTTGTGGTTGGTGGTTGAGGTCTTCACACAGTTCAGTGTCTGGAGCTGGAGCGG CAGCAGATCAGCCAGTTGACTTGGAAACAAAGATGACTGTAAAGCGCACCCATCACCTTCTCAGATGCTC ACTGGTTGGGAGAAGATAGTATTGCTGCATACCCCGTGCAGCTTGTGGTGTGCTCCTCTTTGGGTCTTGT GTTAGGGTCAGGGCCTCTTACGTGGGGCTCTCTAGGGAGAGATTCTTAAATAAATGGAGTCAGCACTATT TTCTGCATCCAGTGTGGTTGCATTTCTCACCTAAGAGCAACGAAGTTCACTCTCTTTACTCTCCTTGGTG TGCTGGATGAAATGTCTCTGTTCTTTGGAGACATGGCTGGAATGAGAAATTTAGTGAGTTTCAATTTGGG CAATTTTTCTGCTTTTTGTAAAGTTCTGGGCAACTGTCTCCTGTAACTGTTGGTTCAGGCCATTGAGCTT TGTTAGGGACAAATGTGCATTTGTTTGTGGCCAAGGCCTAAATTGACAAACTTGTAAATCTCTGTATATA GATTAGCTGTAACCCGACTGTTCTTTGTTTACTGTTTTATATATTATGGGGTTTTTTTTTCCTTTGAAAA TGAAAGGTGTTGGTTTAGGATGGCACTTTGAATTATGTAAATCAGTGAATGGTAGATTTCCTTTACATCT GTCCTTTTCTTCCTAATAGTTTTTATTTTTGAAGTGCCTCCCACATAGGGTTAGGCCATGACTATTTAGG GTATGAGTACCTAACTTCTTATGACTCAGATATGTTGGGAAGTACAGTAACTCCTGGGAATTAACCTTAA TAGCAGATCCGCATGTGAAATGTTCTGACATTGTTAAGTAAGGGTAAGGGGACTGACCAGTGGTCTCCTT TGCCCTTGAACAGTTGTCATTTAATCGCAAAGACCTGTTAACTACTGGTTTAATCGAATCAGTCCTTAAT TCTTGCATGTTTGTATCTAATTTTTAAAAGAAAGAATAAGTATATGTTAACAATATGTATACTTTTTCTT TTAATCAGAGATTCTGAAAACTTCATGTTATTTTAATTTAGATTTGGTGAATTTAAGGGTTTTGAGCATG AAGCTGGCACATAGTGTCTGCAGGATTATTATTCTTTAATCATGGCTAGCTATCTAGTAACGTCATAGAT TTTCAACAGTCTTTTGTTACCTTGCTTTCCTCCAGTTCTACATAGCTTTTGAACTTTGAGCTGAGGGGGG AGAGAGAAGAGGGAGCTTGAAACATTTTCCTTTCTAGAACTCCAGTGTGAGCTTGTGAGGTAGTCTTTTA GCACTATATAGAGTGGACCCAGGGAGGCCCAAGAACCAGAGGCCCAAGAGTGTGCTGAAATGTGCCGCCT CCTTAGGGTGCGAACCTTTGCGATAGGATGTGCTGTGAAACCTAAGTCAAGGTTGGTTTTGAAGGCTGGG GTGAGGGGCAAAATTGTTACTAGATATTAAGACCGTTTAATGTTCCTTAGCGTTCTGAATCCATCTCATG CCTCAGTGAGAATTACAAGGTTTTATTCAGAGCGTTTCAGAGTTTCATACGGAATCAGGAATTGAACAAG TTGCTTTTTGTTTAATGTTGGCTTTCAATAATCACATGGGATGATCTTCTGGACATAAGTGAAAACATGT CCAGGTCTCTACTGTCATTGCTGTAGGATAAAGTGCTCTGCCATCTTCCATGGTTATGAATATTTATTAA TGTTGGAATAACATTTCATTGTTTAGATCACAGCTCAGTTCCTATAGAAAACGAACTGTAAACCTATCTG CAGACCATGCAAGAGGCACAATAAAACTTGAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6515250 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6515250 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17234 same embryo
	EMAGE:17237 same embryo
	EMAGE:17232 same embryo
	EMAGE:17235 same embryo
	EMAGE:17233 same embryo
	EurExpress:euxassay_001624 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source