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EMAGE:17276

Thyn1 thymocyte nuclear protein 1 ( MGI:1925112)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5687
Entity Detected:Thyn1, thymocyte nuclear protein 1 ( MGI:1925112)
Sequence:sense strand is shown

>T5687
GCTCCCAGAAGATTAGCCTGGCAGTGCTTCAGGCGTTTGGGTGGTTTTAGAAGACAAGAGCTGTTTCTTA
CCCGTCCGGTTAGCTTCAGCCTCCTACAGAGAGGCCATGCCGAGACCCCGCAAGAGGCAGACTGGGACTG
CTGGTCCAGACAGGAAGAAGCTATCCGGGAAACGTACTAAAACTGAAAACTCAGAAAGTACATCAGTCAA
GCTAGAGAACTCCAGCCTTGAGATGACGACAACCTTTAAAAATGGTGGCAAGAATTTAAGCAACTACTGG
CTGATGAAGTCAGAGCCAGAAAGCCGGCTAGAGAAAGGTATAGACATGAAGTTCAGCATCGAGGATCTCA
AAGCACAGCCCAAGCAGACAGCTTGCTGGGATGGTGTTCGCAACTATCAGGCTCGGAACTTCCTCAGGGC
CATGAAATTGGAGGATGAAGCCTTCTTCTACCATAGCAACTGCAAACAGCCTGGCATTGTTGGACTTATG
AAGATTGTAAAGGAGGCTTACCCAGACCATACACAGTTTGAGAAAAGTAACCCCCATTACGATCCATCCA
GCAAAGAGGACGACCCTAAATGGTCGATGGTGGATGTACAGTTTGTTCGAATGATGAAGCGCTTCATCCC
TCTGGAGGAGCTTAAAACCTATCACCAAGCCCACAAAGCTACTGGCGGCCCCTTAAAAAGCATGACTCTC
TTCACCCGCCAGAGACTTTCCGTTCAGCCTCTGACTCAAGAAGAGTTTGATTTTATTTTGAGCCTGGAGG
AAACAGAACCAAGTTAATCAGAAGGCTCTACTGATGGAGTGAACAAGCCAAGATTTTATAAGACAGAAGG
AGAAAGCTTGCTGTATCCACCTGGGGTCGTGTGCACGGATGGTTTGTTAATTTTCCTAATAAAAGATTTA
ATGACAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:2631627 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2631627 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17273 same embryo
 EMAGE:17272 same embryo
 EMAGE:17271 same embryo
 EMAGE:17274 same embryo
 EMAGE:17275 same embryo
 EurExpress:euxassay_003677 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS