EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:17272

Psmd5 proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 ( MGI:1914248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001649. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001649_01	euxassay_001649_02	euxassay_001649_03	euxassay_001649_04

euxassay_001649_05	euxassay_001649_06	euxassay_001649_07	euxassay_001649_08	euxassay_001649_09

euxassay_001649_10	euxassay_001649_11	euxassay_001649_12	euxassay_001649_13	euxassay_001649_14

euxassay_001649_15	euxassay_001649_16	euxassay_001649_17	euxassay_001649_18	euxassay_001649_19

euxassay_001649_20	euxassay_001649_21	euxassay_001649_22	euxassay_001649_23	euxassay_001649_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17272_wholemount_strong.wlz

17272_wholemount_moderate.wlz

17272_wholemount_weak.wlz

17272_wholemount_possible.wlz

17272_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17272_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5685
Entity Detected:	Psmd5, proteasome (prosome, macropain) 26S subunit, non-ATPase, 5 ( MGI:1914248)
Sequence:	sense strand is shown >T5685 CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGGAGATGGCGGCGCAGGCTGTGT CGCTCCTGCGGGAGGTGGCGCGGCTGGAGGCGCCGCTGGAGGAGCTTCGTGCGCTGCAGTCAGTGGTGCA GGCTGTGCCGCTGCACGAGCTTCGCGAGCAGGCGGCGGAGCTACGCCTCCGCCCGCTGTTCTCCTTGCTT AACCAGAACAATCGGGAACAGACTGCTTTGTGTGTCTCCATTCTGGAGAGGCTGCTCCAAGCTGTGGAGC CGATTCACTTGGCCAGGAACCTCAGGCTTGACCTGCAGAGGGGACTGACTCACCCTGATGACTCTGTAAA AACCCTCACTCTGTCGCAGATTGGAAGAATTGTTGAAAATTCAGAAGCTGTTACTGAGATTCTCAATAAT GCTGAGCTGTTGAAACAGATTGTTTATTGCATTGGCGGAGAGAATTTATCTGTTGCTAAAGCGGCTATTA AATCCCTGTCGAGAATATCACTAACCCAGGCTGGCCTGGAAGCTCTGTTTGAAAGTAACCTGCTGGATGA TTTGAAGAATGTAATGAAAACAAACGACGTTGTTCGATACAGGGTCTATGAGCTAATTATAGATATTTCT TCTGTGTCATCAGAATCTTTAAACTACTGTACCACAAGTGGACTGGTGACTCAACTCCTAAAAGAGCTGA CGGGTGAGGACGTGTTAGTCAGAGCCACCTGTATAGAAATGGTGACATCACTAGCTTATACCCATCACGG ACGACAATACCTTGCTCAAGAAGGAGTCATTGACCAGATATCCAATATAATTGTTGGCGCAGATTCAGAC CCTTTCTCTGGCTTCTATCTGCCAGGATTTGTGAAGTTTTTTGGAAACCTGGCCGTCATGGATAGTCCTC AGCAGATCTGTGAGCGCTACCCTGTTTTTCTGGAGAAGGTGTTTGAAATGGCGGATAGTCAAGACCCCAC CATGATTGGTGTCGCTGTCGACACAGTTGGAATCCTGGGATCCAGTGTTGAAGGAAAACAAGTTTTACAG AAGACAGGAACCCGCTTTGAACGTGTCCTCATGAGAGTAGGATATCAAGCAAAGAATGCTTCCACGGAGC TGAAGATTAGGTGCTTGGATGCAGTTTCCTCTCTCCTGTATTTATCGCCTGAGCAGCAGACTGATGACTT CCTGGGAATGACAGAATCCTGGTTTTCCTCCATGTCTCGAGACTCCCTGGAGCCTTTCCGTGGTATCAGT AACCAGCCCTTCCCGGAGCTACACTGTGCTGCCTTGAAAGTTTTCACAGCTATTGCAGACCAGCCCTGGG CTCAGAGACTTATGTTTAACAGTCCAGGTTTTGTAGAGTTTGTGATGGACCGGTCTGTGGAACATGACAA AGCTTCAAAGGATGCCAAATATGAACTGGTAAAAGCACTTGCCAATTCCAAGACAGTTGCAGAGATCTTT GGGAACTCAAATTATTTGAGGCTCAGAGCGTACCTAAGTGAAGGTCCATACTATGTGAAACCTGTTGCCA CAACAGCAGTAGAAGGGGCTGACTGATCCCTTTCCACCTAAAACAGCAGAATTTCCCAAGGCACACGATT CCATCCGTGCTCCACACCACAGGACGTCTGTCTTCCCAGGCACAGCCTCACCATCAGCAGTGTTGTCCCT ACTTAATGCTGTGTAGACTCGGCTCTTGTAAAAGCAGTAGGAGCCTGAGAGTGCTCGTGTTTCCCCGAGT CCTCTGGCTGCCAAAGTCTCTGCTGCAAGGTCGAGGTTTGTTTTCTCAAAGTTTCCATGGGCACACTCAT GACATTTTTAAAAAATAAAATGAAGCCAAAATGATTCTGGATTCTCTCAACCACATTTTCTTTTTCCTTA CCTGATTTTTGCCTCAAAATCTTGGGTATCTTGGGTAGTCTCTAAAAAATAAGTCATTTAGAATCATCGA ATTATATTATTTGTATATAAGGAATAATAGTTTGAATAATATTTCTTACTTTTCCTTTGAAAATAAAATA AATCCAAACTTGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2631141 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2631141 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17273 same embryo
	EMAGE:17271 same embryo
	EMAGE:17276 same embryo
	EMAGE:17274 same embryo
	EMAGE:17275 same embryo
	EurExpress:euxassay_001649 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source